Prevalence and antibiogram of Extended Spectrum β-lactamase (ESBL) producing Gram negative bacilli and further molecular characterization of ESBL producing Escherichia coli and klebsiella spp

Journal of Clinical and Diagnostic Research

Volumn 7, Issue 10, 2013, Pages 2173-2177

  Sharma, Meeta ^a   Pathak, Sati ^a   Srivastava, Preeti ^a  

^a NIMS UNIVERSITY   (India)

Author keywords

Antibiogram; Double disk synergy test (DDST); Extended spectrum lactamase (ESBL); PCR; Plasmid

Indexed keywords

AMIKACIN; CEFEPIME; CEFOTAXIME; CEFTAZIDIME; CIPROFLOXACIN; CLAVULANIC ACID; GENTAMICIN; PIPERACILLIN PLUS TAZOBACTAM;

ACINETOBACTER; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM ISOLATE; BLA CTX M GENE; BLA SHV GENE; BLA TEM GENE; BLOOD; BODY FLUID; CITROBACTER FREUNDII; CONTROLLED STUDY; DOUBLE DISK SYNERGY TEST; EXTENDED SPECTRUM BETA LACTAMASE PRODUCING ESCHERICHIA COLI; EXTENDED SPECTRUM BETA LACTAMASE PRODUCING KLEBSIELLA PNEUMONIAE; FECES; GENE EXPRESSION; GENE IDENTIFICATION; GRAM NEGATIVE BACTERIUM; MICROBIOLOGICAL EXAMINATION; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PROTEUS; PSEUDOMONAS; PUS; RESPIRATORY SYSTEM; SALMONELLA PARATYPHI; SALMONELLA TYPHI; SCREENING TEST; SPECIES DIFFERENCE; STRAIN IDENTIFICATION; URINE;

EID: 84885196665     PISSN: 2249782X     EISSN: 0973709X     Source Type: Journal    
DOI: 10.7860/JCDR/2013/6460.3462     Document Type: Article

References (34)

1. Bradford PA. "Extended-spectrum β-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat." Clin Microbiol Rev. 14.2001; 48:933-51.
2. Giraud-Morin C, Fosse TA. Seven-year survey of Klebsiella pneumoniae producing TEM-24 extended-spectrum-lactamase in Nice University Hospital (1994-2000). J. Hosp. Infect. 2003; 54: 25-31.
3. Paterson DL, Bonomo RA. Extended spectrum β-lactamases: a clinical update. Clin Microbiol Rev. 2005; 18: 657-86.
4. Nathisuwan S, Burgess DS, Lewis JS. 2nd Extended Spectrum β-lactamases: Epidemiology, Detection and treatment. Pharmacotherapy. 2001; 21: 920-8.
5. Rupp ME, Fey PD. Extended Spectrum β-lactamase (ESBL) - Producing Enterobacteriaceae. Drugs. 2003; 63: 353-65.
6. Poole K. Resistance to β -Lactam antibiotics. Cell. Mol. Life Sci. 2004; 61: 2200-23.
7. Bonnet R. "Growing group of extended-spectrum β-lactamases: the CTX-M enzymes," Antimicrobial Agents and Chemotherapy. 2004; 48(1):1-14.
8. Perez F, Endimiani A, Hujer KM, and Bonomo RA. "The continuing challenge of ESBLs," Current Opinion in Pharmacology. 2007; 7(5): 459-69.
9. Paterson DL, Hujer KM, Hujer AM, Yeiser B, Bonomo MD, Rice LB, et al., "Extended-spectrum b-lactamases in Klebsiella pneumoniae bloodstream isolates from seven countries: dominance and widespread prevalence of SHV-and CTX-M-type b-lactamases." Antimicrob Agents Chemother. 2003; 47: 3554-60.
10. Jain A, Mondal R. TEM & SHV genes in extended spectrum _-lactamase producing Klebsiella species & their antimicrobial resistance pattern. Indian J. Med. Res. 2008; 128: 759-64.
11. Clinical Laboratory Standards Institute. Performance standards for antimicrobial disc susceptibility tests, IXX international Supplement (M100- S19). Wayne Pa, USA: National Committee for Clinical Laboratory Standards. 2009.
12. Gopalakrishnan R, Sureshkumar D: Changing Trends in Antimicrobial Susceptibility and Hospital Acquired Infections Over an 8 Year Period in a Tertiary Care Hospital in Relation to Introduction of an Infection Control Programme. J Assoc Physicians India. 2010 December; 58 Suppl: 25-31.
13. Shanthi M, Sekar U. Extended Spectrum Beta Lactamase Producing Escherichia Coli and Klebsiella Pneumoniae: Risk Factors for Infection and Impact of Resistance on Outcomes. J Assoc Physicians India. 2010 December; 58 Suppl: 41-4.
14. Vemula S and Vadde R. Prevalence of ESBL-Producing Klebsiella pneumoniae Isolates in Tertiary Care Hospital. ISRN Microbiology. 2011; 2011: 318-48.
15. Mubarak SA, Abida AE, Hajar MA, Adeel IA. Molecular Characterization and Epidemiology of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in the United Arab Emirates. Med Princ Pract. 2011; 20:177-80.
16. Ali AM, Rafi S, Qureshi AH. Frequency of Extended spectrum beta Lactamase producing gram negative bacilli among clinical isolates at clinical laboratories of Army medical college, Rawalpindi. J Ayub Med Coll Abbottabad. 2004 Jan- Mar; 16(1): 35-7.
17. Patel BV, Patel PG, Raval PN, Patel MH, Patel PH, Vegad MM. Bacteriological profile and antibiogram of gram negative organisms isolated from medical and neurology intensive care unit with special reference to multi-drug resistant organisms. National journal of Medical Research. 2012 July-Sept; 2(3): 335-38.
18. Iraj A, Nilufar YN. Antibiogram of Extended Spectrum Beta-lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae isolated from Hospital Samples. Bangladesh J Med Microbiol. 2010; 04 (01): 32-6.
19. Saba R, Muhammad F and Shahida H. Prevalence and comparison of Beta-lactamase producing Escherichia coli and Klebsiella spp. from clinical and environmental sources in Lahore, Pakistan. African Journal of Microbiology Research. 2012 January; 6(2): 465-70.
20. Abhilash KP, Veeraraghavan B, Abraham OC: Epidemiology and Outcome of Bacteremia Caused by Extended Spectrum Beta-Lactamase (ESBL)-producing Escherichia Coli and Klebsiella Spp. in a Tertiary Care Teaching Hospital in South India. J Assoc Physicians India. 2010 December; 58 Suppl:13-7.
21. Wattal C, Goel N, Oberoi JK, Raveendran RS, Datta S, Prasad K J. Surveillance of Multidrug Resistant Organisms in a Tertiary Care Hospital in Delhi, India. J Assoc Physicians India. 2010 Dec; 58 Suppl: 32-6.
22. Umadevi S, Kandhakumari G, Joseph NM, Kumar S, Easow JM, Stephen S, et al., Prevalence and antimicrobial Susceptibility pattern of ESBL producing Gram Negative Bacilli. J Clin Diagn Res. 2011 Apr; 5(2):236-39
23. Ava B, Mohammad R and Jalil V and Yousefi. Frequency of extended spectrum beta-lactamase (ESBLs) producing Escherichia coli and klebseilla pneumonia isolated from urine in an Iranian 1000-bed tertiary care hospital. African Journal of Microbiology Research. 2010 May; 4 (9): 881-84.
24. Nuesch-Inderbinen MT, Hachler FHKH. Detection of genes 15 coding for extended-spectrum SHV beta-lactamases in clinical isolates by a molecular genetic method, and comparison with the Etest. Eur J Clin Microbiol Infect Dis. 1996; 15: 398-402.
25. Sharma J, Sharma M & Ray P: Detection of TEM & SHV genes in Escherichia coli & Klebsiella pneumoniae isolates in a tertiary care hospital from India. Indian J Med Res. 2010 September; 132: 332-36.
26. Bali EB, Ac. KL, Sultan N: Phenotypic and molecular characterization of SHV, TEM, CTX-M and extended-spectrum-lactamase produced by Escherichia coli, Acinobacter baumannii and Klebsiella isolates in a Turkish hospital. African Journal of Microbiology Research. 2010 April; 4 (8): 650-54.
27. Vaida S, Marius L, Aurelija B, Justas P, Rita P, Agne G et al., Molecular characterization of extended-spectrum b-lactamase producing Escherichia coli and Klebsiella pneumoniae isolates from hospitals in Lithuania. Journal of Medical Microbiology. 2010; 59 (10): 1263-65.
28. Livermore DM, Canton R, Gniadkowski M, Nordmann P, Rossolini GM, Arlet G, et al., CTX-M: changing the face of ESBLs in Europe. J Antimicrob Chemother. 2007 Feb; 59:165-74.
29. Bonnet R. Growing group of extended-spectrum beta-lactamases: the CTX-M enzymes. Antimicrob Agents Chemother. 2004; 48:1-14.
30. Lim KT, Yasin R, Yeo CC, Puthucheary S, Thong KL. Characterization of Multidrug Resistant ESBL-Producing Escherichia coli Isolates from Hospitals in Malaysia. J Biomed Biotechnol. 2009; 165637:10 pages.
31. Pattarachai K, Anucha A, Chaitat L, and Piyawan S. Molecular Characterization and Epidemiology of Extended-Spectrum- β-Lactamase-Producing Escherichia coli and Klebsiella pneumonia Isolates Causing Health Care-Associated Infection in Thailand, Where the CTX-M Family Is Endemic. Antimicrobial agents and chemotherapy. 2008 Aug; 52(8): 2818-24.
32. Nazik H, Bektore B, Ongen B, lktaç M, Ozyurt M, Nuray KN, et al., Plasmid- Mediated Quinolone Resistance Genes in Escherichia coli Urinary Isolates from Two Teaching Hospitals in Turkey: Coexistence of TEM, SHV, CTX-M and VEB-1 Type β-lactamases. Tropical Journal of Pharmaceutical Research. 2011 June; 10 (3): 325-33.
33. Goyal A, Prasad KN, Prasad A, Gupta S, Ghoshal U, Ayyagari A. Extended spectrum beta-lactamases in Escherichia coli & Klebsiella pneumoniae & associated risk factors. Indian J Med Res. 2009 Jun; 129(6): 695-700.
34. Gupta V. An update on newer β-lactamases. Indian J. Med. Res. 2007; 126: 417-27.