Long- and short-term intravital imaging reveals differential spatiotemporal recruitment and function of myelomonocytic cells after spinal cord injury

Journal of Physiology

Volumn 591, Issue 19, 2013, Pages 4895-4902

  Fenrich, Keith K ^a   Weber, Pascal ^a   Rougon, Geneviève ^a,b   Debarbieux, Franck ^a,b  

^a AIX MARSEILLE UNIVERSITÉ   (France)

^b Aix Marseille University   (France)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOPROTEIN P 15095; LYSOZYME; LYSOZYME M; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL INFILTRATION; CELL INTERACTION; CELL MOTILITY; CELL POPULATION; CELL STRUCTURE; CONTROLLED STUDY; GRANULOCYTE; IMAGE ANALYSIS; IN VIVO STUDY; INFLAMMATION; MONOCYTE; MOUSE; MULTIPHOTON MICROSCOPY; MYELOMONOCYTIC CELL; NERVE FIBER; NEUROPHYSIOLOGICAL RECRUITMENT; NEUTROPHIL; NONHUMAN; PHAGOCYTOSIS; PRIORITY JOURNAL; SPINAL CORD INJURY; SPINAL GANGLION;

ANIMALS; ANTIGENS, CD11C; AXONS; CELL MOVEMENT; MICE; MICE, INBRED C57BL; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; MONOCYTES; MURAMIDASE; OPTICAL IMAGING; PHAGOCYTOSIS; SPINAL CORD INJURIES;

EID: 84884977185     PISSN: 00223751     EISSN: 14697793     Source Type: Journal    
DOI: 10.1113/jphysiol.2013.256388     Document Type: Article

References (25)

1. Bulloch K, Miller MM, Gal-Toth J, Milner TA, Gottfried-Blackmore A, Waters EM, Kaunzner UW, Liu K, Lindquist R, Nussenzweig MC, Steinman RM & McEwen BS (2008). CD11c/EYFP transgene illuminates a discrete network of dendritic cells within the embryonic, neonatal, adult, and injured mouse brain. J Comp Neurol 508, 687-710.
2. Busch SA, Hamilton JA, Horn KP, Cuascut FX, Cutrone R, Lehman N, Deans RJ, Ting AE, Mays RW & Silver J (2011). Multipotent adult progenitor cells prevent macrophage-mediated axonal dieback and promote regrowth after spinal cord injury. J Neurosci 31, 944-953.
3. Busch SA, Horn KP, Silver DJ & Silver J (2009). Overcoming macrophage-mediated axonal dieback following CNS injury. J Neurosci 29, 9967-9976.
4. Chow A, Brown BD & Merad M (2011). Studying the mononuclear phagocyte system in the molecular age. Nat Rev Immunol 11, 788-798.
5. Cross M, Mangelsdorf I, Wedel A & Renkawitz R (1988). Mouse lysozyme M gene: isolation, characterization, and expression studies. Proc Natl Acad Sci U S A 85, 6232-6236.
6. Dray C, Rougon G & Debarbieux F (2009). Quantitative analysis by in vivo imaging of the dynamics of vascular and axonal networks in injured mouse spinal cord. Proc Natl Acad Sci U S A 106, 9459-9464.
7. Faust N, Varas F, Kelly LM, Heck S & Graf T (2000). Insertion of enhanced green fluorescent protein into the lysozyme gene creates mice with green fluorescent granulocytes and macrophages. Blood 96, 719-726.
8. Feng G, Mellor RH, Bernstein M, Keller-Peck C, Nguyen QT, Wallace M, Nerbonne JM, Lichtman JW & Sanes JR (2000). Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28, 41-51.
9. Fenrich KK & Rose PK (2011). Axons with highly branched terminal regions successfully regenerate across spinal midline transections in the adult cat. J Comp Neurol 519, 3240-3258.
10. Fenrich KK, Weber P, Hocine M, Zalc M, Rougon G & Debarbieux F (2012). Long-term in vivo imaging of normal and pathological mouse spinal cord with subcellular resolution using implanted glass windows. J Physiol 590, 3665-3675.
11. Horn KP, Busch SA, Hawthorne AL, Van Rooijen N & Silver J (2008). Another barrier to regeneration in the CNS: activated macrophages induce extensive retraction of dystrophic axons through direct physical interactions. J Neurosci 28, 9330-9341.
12. Hume DA (2008). Macrophages as APC and the dendritic cell myth. J Immunol 181, 5829-5835.
13. Hume DA (2011). Applications of myeloid-specific promoters in transgenic mice support in vivo imaging and functional genomics but do not support the concept of distinct macrophage and dendritic cell lineages or roles in immunity. J Leukoc Biol 89, 525-538.
14. Kettenmann H, Hanisch U-K, Noda M & Verkhratsky A (2011). Physiology of microglia. Physiol Rev 91, 461-553.
15. Laliberte AM & Fehlings MG (2013). The immunological response to spinal cord injury: helpful or harmful Exp Neurol 247, 282-285.
16. Lindquist RL, Shakhar G, Dudziak D, Wardemann H, Eisenreich T, Dustin ML & Nussenzweig MC (2004). Visualizing dendritic cell networks in vivo. Nat Immunol 5, 1243-1250.
17. Mawhinney LA, Thawer SG, Lu W-Y, Rooijen N van, Weaver LC, Brown A & Dekaban GA (2012). Differential detection and distribution of microglial and hematogenous macrophage populations in the injured spinal cord of lys-EGFP-ki transgenic mice. J Neuropathol Exp Neurol 71, 180-197.
18. Minagar A, Shapshak P, Fujimura R, Ownby R, Heyes M & Eisdorfer C (2002). The role of macrophage/microglia and astrocytes in the pathogenesis of three neurologic disorders: HIV-associated dementia, Alzheimer disease, and multiple sclerosis. J Neurol Sci 202, 13-23.
19. Neumann H, Kotter MR & Franklin RJM (2009). Debris clearance by microglia: an essential link between degeneration and regeneration. Brain 132, 288-295.
20. Ramon Y & Cajal S (1959). Degeneration and Regenerations of the Nervous System. Hafner, New York .
21. Shechter R, London A, Varol C, Raposo C, Cusimano M, Yovel G, Rolls A, Mack M, Pluchino S, Martino G, Jung S & Schwartz M (2009). Infiltrating blood-derived macrophages are vital cells playing an anti-inflammatory role in recovery from spinal cord injury in mice. PLoS Med 6, e1000113.
22. Shechter R, Raposo C, London A, Sagi I & Schwartz M (2011). The glial scar-monocyte interplay: a pivotal resolution phase in spinal cord repair. PLoS ONE 6, e27969.
23. Stow JL, Low PC, Offenhäuser C & Sangermani D (2009). Cytokine secretion in macrophages and other cells: pathways and mediators. Immunobiology 214, 601-612.
24. Thévenaz P, Ruttimann UE & Unser M (1998). A pyramid approach to subpixel registration based on intensity. IEEE Trans Image Process 7, 27-41.
25. Tom VJ, Steinmetz MP, Miller JH, Doller CM & Silver J (2004). Studies on the development and behaviour of the dystrophic growth cone, the hallmark of regeneration failure, in an in vitro model of the glial scar and after spinal cord injury. J Neurosci 24, 6531-6539.