Continuous enzyme-coupled assay of phosphate- or pyrophosphate-releasing enzymes

BioTechniques

Volumn 53, Issue 2, 2012, Pages 99-103

  Hochkoeppler, Alejandro ^a,b,c   Guillén Suárez, Antonio S ^a,c   Stefan, Alessandra ^a,b   Lemma, Silvia ^a   Conte, Emanuele ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b UNIVERSITY OF FLORENCE   (Italy)

^c UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

Author keywords

Coupled assay; DNA polymerases; Phosphate; Pyrophosphate

Indexed keywords

DNA POLYMERASE; HYPOXANTHINE; INORGANIC PYROPHOSPHATASE; INOSINE; PHOSPHATE; PURINE NUCLEOSIDE PHOSPHORYLASE; PYROPHOSPHATE; URIC ACID; XANTHINE OXIDASE;

ARTICLE; CELLULOMONAS; DNA REPLICATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME ISOLATION; ENZYME KINETICS; ENZYME PURIFICATION; ESCHERICHIA COLI; NONHUMAN; PH; SPECTROPHOTOMETRY;

ANIMALS; CATTLE; DNA; DNA POLYMERASE I; DNA-DIRECTED DNA POLYMERASE; ENZYME ASSAYS; ESCHERICHIA COLI; HYPOXANTHINE; INORGANIC PYROPHOSPHATASE; INOSINE; KINETICS; PHOSPHATES; PURINE-NUCLEOSIDE PHOSPHORYLASE; SPECTROPHOTOMETRY; XANTHINE OXIDASE;

EID: 84865062996     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000113905     Document Type: Article

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