DEER EPR measurements for membrane protein structures via bifunctional spin labels and lipodisq nanoparticles

Biochemistry

Volumn 52, Issue 38, 2013, Pages 6627-6632

  Sahu, Indra D ^a   McCarrick, Robert M ^a   Troxel, Kaylee R ^a   Zhang, Rongfu ^a   Smith, Hubbell J ^a   Dunagan, Megan M ^a   Swartz, Max S ^a   Rajan, Prashant V ^a   Kroncke, Brett M ^b   Sanders, Charles R ^b   Lorigan, Gary A ^a  

^a MIAMI UNIVERSITY   (United States)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DISTANCE DISTRIBUTIONS; GLOBULAR PROTEINS; INFORMATION CONTENTS; INTEGRAL MEMBRANE PROTEINS; MEMBRANE PROTEIN STRUCTURES; SIGNAL SENSITIVITY; STRUCTURAL STUDIES; TRANSVERSE RELAXATION TIME;

LIPID BILAYERS; NANOPARTICLES; PROTEINS; RELAXATION TIME;

ELECTRON SPIN RESONANCE SPECTROSCOPY;

MEMBRANE PROTEIN; NANOPARTICLE; POTASSIUM CHANNEL KCNE1; PROTEOLIPOSOME;

ARTICLE; DOUBLE ELECTRON ELECTRON RESONANCE EPR SPECTROSCOPY; ELECTRON SPIN RESONANCE; MEMBRANE; MICELLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; RELAXATION TIME; SPIN LABELING;

CYCLIC N-OXIDES; ELECTRON SPIN RESONANCE SPECTROSCOPY; LIPID BILAYERS; MEMBRANE PROTEINS; MESYLATES; NANOPARTICLES; POTASSIUM CHANNELS, VOLTAGE-GATED; SPIN LABELS;

EID: 84884890933     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi4009984     Document Type: Article

References (31)

1. Borbat, P. P., Mchaourab, H. S., and Freed, J. H. (2002) Protein structure determination using long-distance constraints from double-quantum coherence ESR: Study of T4 lysozyme J. Am. Chem. Soc. 124, 5304-5314
2. Jeschke, G. and Polyhach, Y. (2007) Distance measurements on spin-labelled biomacromolecules by pulsed electron paramagnetic resonance Phys. Chem. Chem. Phys. 9, 1895-1910
3. Mchaourab, H. S., Steed, P. R., and Kazmier, K. (2011) Toward the Fourth Dimension of Membrane Protein Structure: Insight into Dynamics from Spin-Labeling EPR Spectroscopy Structure 19, 1549-1561
4. Jeschke, G. (2012) DEER Distance Measurements on Proteins Annu. Rev. Phys. Chem. 63, 419-446
5. Zou, P., Bortolus, M., and Mchaourab, H. S. (2009) Conformational Cycle of the ABC Transporter MsbA in Liposomes: Detailed Analysis Using Double Electron-Electron Resonance Spectroscopy J. Mol. Biol. 393, 586-597
6. Endeward, B., Butterwick, J. A., MacKinnon, R., and Prisner, T. F. (2009) Pulsed Electron-Electron Double-Resonance Determination of Spin-Label Distances and Orientations on the Tetrameric Potassium Ion Channel KcsA J. Am. Chem. Soc. 131, 15246-15250
7. Georgieva, E. R., Ramlall, T. F., Borbat, P. P., Freed, J. H., and Eliezer, D. (2008) Membrane-bound alpha-synuclein forms an extended helix: Long-distance pulsed ESR measurements using vesicles, bicelles, and rodlike micelles J. Am. Chem. Soc. 130, 12856-12857
8. Zou, P. and Mchaourab, H. S. (2010) Increased Sensitivity and Extended Range of Distance Measurements in Spin-Labeled Membrane Proteins: Q-Band Double Electron-Electron Resonance and Nanoscale Bilayers Biophys. J. 98, L18-L20
9. Xu, Q., Ellena, J. F., Kim, M., and Cafiso, D. S. (2006) Substrate-dependent unfolding of the energy coupling motif of a membrane transport protein determined by double electron-electron resonance Biochemistry 45, 10847-10854
10. Dastvan, R., Bode, B. E., Karuppiah, M. P. R., Marko, A., Lyubenova, S., Schwalbe, H., and Prisner, T. F. (2010) Optimization of Transversal Relaxation of Nitroxides for Pulsed Electron-Electron Double Resonance Spectroscopy in Phospholipid Membranes J. Phys. Chem. B 114, 13507-13516
11. Hilger, D., Polyhach, Y., Jung, H., and Jeschke, G. (2009) Backbone Structure of Transmembrane Domain IX of the Na+/Proline Transporter PutP of Escherichia coli Biophys. J. 96, 217-225
12. Fleissner, M. R., Bridges, M. D., Brooks, E. K., Cascio, D., Kálai, T., Hideg, K., and Hubbell, W. L. (2011) Structure and dynamics of a conformationally constrained nitroxide side chain and applications in EPR spectroscopy Proc. Natl. Acad. Sci. U. S. A. 108, 16241-16246
13. Orwick-Rydmark, M., Lovett, J. E., Graziadei, A., Lindholm, L., Hicks, M. R., and Watts, A. (2012) Detergent-Free Incorporation of a Seven-Transmembrane Receptor Protein into Nanosized Bilayer Lipodisq Particles for Functional and Biophysical Studies Nano Lett. 12, 4687-4692
14. Polyhach, Y., Bordignon, E., Tschaggelar, R., Gandra, S., Godt, A., and Jeschke, G. (2012) High sensitivity and versatility of the DEER experiment on nitroxide radical pairs at Q-band frequencies Phys. Chem. Chem. Phys. 14, 10762-10773
15. Höfer, P., Heilig, R., and Schmalbein, D. (2003) The superQ-FT accessory for pulsed EPR, ENDOR and ELDOR at 34 GHz Bruker Spin Report 37-43
16. Ghimire, H., McCarrick, R. M., Budil, D. E., and Lorigan, G. A. (2009) Significantly Improved Sensitivity of Q-Band PELDOR/DEER Experiments Relative to X-Band Is Observed in Measuring the Intercoil Distance of a Leucine Zipper Motif Peptide (GCN4-LZ) Biochemistry 48, 5782-5784
17. Orwick, M. C., Judge, P. J., Procek, J., Lindholm, L., Graziadei, A., Engel, A., Grobner, G., and Watts, A. (2012) Detergent-Free Formation and Physicochemical Characterization of Nanosized Lipid-Polymer Complexes: Lipodisq Angew. Chem., Int. Ed. 51, 4653-4657
18. Kang, C., Tian, C., Sonnichsen, F. D., Smith, J. A., Meiler, J., George, A. L. J., Vanoye, C. G., Kim, H. J., and Sanders, C. R. (2008) Structure of KCNE1 and Implications for How It Modulates the KCNQ1Potassium Channel Biochemistry 47, 7999-8006
19. Tian, C., Vanoye, C. G., Kang, C., Welch, R. C., Kim, H. J., George, A. L., and Sanders, C. R. (2007) Preparation, Functional Characterization, and NMR Studies of Human KCNE1, a Voltage-Gated Potassium Channel Accessory Subunit Associated with Deafness and Long QT Syndrome Biochemistry 46, 11459-11472
20. Coey, A. T., Sahu, I. D., Gunasekera, T. S., Troxel, K. R., Hawn, J. M., Swartz, M. S., Wickenheiser, M. R., Reid, R.-j., Welch, R. C., Vanoye, C. G., Kang, C., Sanders, C. R., and Lorigan, G. A. (2011) Reconstitution of KCNE1 into Lipid Bilayers: Comparing the Structural, Dynamic, and Activity Differences in Micelle and Vesicle Environments Biochemistry 50, 10851-10859
21. Melman, Y. F., Um, S. Y., Krumerman, A., Kagan, A., and McDonald, T. V. (2004) KCNE1 binds to the KCNQ1 pore to regulate potassium channel activity Neuron 42, 927-937
22. Panaghie, G., Tai, K. K., and Abbott, G. W. (2006) Interaction of KCNE subunits with the KCNQ1 K+ channel pore J. Physiol. 570, 455-467
23. Li, G.-R., Feng, J., Yue, L., Carrier, M., and Nattel, S. (1996) Evidence for two components of delayed rectifi er K + current in human ventricular myocytes Circ. Res. 78, 689-696
24. Jost, N., Virag, L., Bitay, M., Takacs, J., Lengye, l. C., Biliczki, P., Nagy, Z., Bogats, G., Lathrop, D. A., Papp, J. G., and Varro, A. (2005) Restricting excessive cardiac action potential and QT prolongation. A vital role for I Ks in human ventricular muscle Circulation 112, 1392-1399
25. Wang, Z., Fermini, B., and Nattel, S. (1994) Rapid and slow components of delayed rectifi er current in human atrial myocytes Cardiovasc. Res. 28, 1540-1546
26. Harmer, S. C. and Tinker, A. (2007) The role of abnormal trafficking of KCNE1 in long QT syndrome 5 Biochem. Soc. Trans. 35, 1074-1076
27. Barrett, P. J., Song, Y., Van Horn, W. D., Hustedt, E. J., Schafer, J. M., Hadziselimovic, A., Beel, A. J., and Sanders, C. R. (2012) The Amyloid Precursor Protein Has a Flexible Transmembrane Domain and Binds Cholesterol Science 336, 1168-1171
28. Feldmann, E. A., Ni, S., Sahu, I. D., Mishler, C. H., Risser, D. D., Murakami, J. L., Tom, S. K., McCarrick, R. M., Lorigan, G. A., Tolbert, B. S., Callahan, S. M., and Kennedy, M. A. (2011) Evidence for Direct Binding between HetR from Anabaena sp PCC 7120 and PatS-5 Biochemistry 50, 9212-9224
29. Jeschke, G., Chechik, V., Ionita, P., Godt, A., Zimmermann, H., Banham, J., Timmel, C. R., Hilger, D., and Jung, H. (2006) DeerAnalysis2006-a comprehensive software package for analyzing pulsed ELDOR data Appl. Magn. Reson. 30, 473-498
30. Chiang, Y. W., Borbat, P. P., and Freed, J. H. (2005) The determination of pair distance distributions by pulsed ESR using Tikhonov regularization J. Magn. Reson. 172, 279-295
31. Moen, R. J., Thomas, D. D., and Klein, J. C. (2013) Conformationally Trapping the Actin-binding Cleft of Myosin with a Bifunctional Spin Label J. Biol. Chem. 288, 3016-3024