Construction, characterization and application of molecular tools for metabolic engineering of Synechocystis sp.

Biotechnology Letters

Volumn 35, Issue 10, 2013, Pages 1655-1661

  Qi, Fengxia ^a,b   Yao, Lun ^a,b   Tan, Xiaoming ^a   Lu, Xuefeng ^a  

^a QINGDAO INSTITUTE OF BIOENERGY AND BIOPROCESS TECHNOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Fatty acyl CoA reductase; Fatty alcohol; Promoters; Synechocystis sp. PCC6803; Synthetic biology

Indexed keywords

FATTY ACYL-COA REDUCTASE; FATTY ALCOHOLS; PROMOTERS; SYNECHOCYSTIS SP; SYNTHETIC BIOLOGY;

ENZYME ACTIVITY; GENE EXPRESSION; GENETIC ENGINEERING;

ALCOHOLS;

SYNECHOCYSTIS; SYNECHOCYSTIS SP.;

FATTY ALCOHOL;

ARTICLE; GENE DOSAGE; GENE EXPRESSION; GENE INACTIVATION; GENETICS; METABOLIC ENGINEERING; METABOLISM; METHODOLOGY; MICROBIAL GENETICS; MICROBIOLOGY; MOLECULAR BIOLOGY; PROMOTER REGION; SYNECHOCYSTIS;

FATTY ALCOHOLS; GENE DOSAGE; GENE EXPRESSION; GENE KNOCKOUT TECHNIQUES; GENETICS, MICROBIAL; INDUSTRIAL MICROBIOLOGY; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; MOLECULAR BIOLOGY; PROMOTER REGIONS, GENETIC; SYNECHOCYSTIS;

EID: 84884818081     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-013-1252-0     Document Type: Article

References (15)

1. Boyle PM, Silver PA (2012) Parts plus pipes: synthetic biology approaches to metabolic engineering. Metab Eng 14(3): 223-232.
2. Ducat DC, Way JC, Silver PA (2011) Engineering cyanobacteria to generate high-value products. Trends Biotechnol 29(2): 95-103.
3. Elhai J, Wolk CP (1988) A versatile class of positive-selection vectors based on the nonviability of palindrome containing plasmids that allows cloning into long polylinkers. Gene 68(1): 119-138.
4. Gao H, Tang Q, Xu X (2007) Construction of copper-induced gene expression platform in Synechocystis sp. PCC 6803. Acta Hydrobiol Sin 31(2): 240-244.
5. Gao Z, Zhao H, Li Z, Tan X, Lu X (2012) Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria. Energy Environ Sci 5(12): 9857-9865.
6. Huang HH, Camsund D, Lindblad P, Heidorn T (2010) Design and characterization of molecular tools for a synthetic biology approach towards developing cyanobacterial biotechnology. Nucleic Acids Res 38(8): 2577-2593.
7. Liu X, Sheng J, Curtiss III R (2011) Fatty acid production in genetically modified cyanobacteria. Proc Natl Acad Sci USA 108(17): 6899-6904.
8. Matteucci M, Heyneker H (1983) Targeted random mutagenesis: the use of ambiguously synthesized oligonucleotides to mutagenize sequences immediately 5′ of an ATG initiation codon. Nucleic Acids Res 11(10): 3113-3121.
9. Schaefer MR, Golden SS (1989) Differential expression of members of a cyanobacterial psbA gene family in response to light. J Bacteriol 171(7): 3973-3981.
10. Stanier RY, Kunisawa R, Mandel M, Cohen-Bazire G (1971) Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev 35(2): 171-205.
11. Takeshima Y, Takatsugu N, Sugiura M, Hagiwara H (1994) High-level expression of human superoxide dismutase in the cyanobacterium Anacystis nidulans 6301. Proc Natl Acad Sci USA 91(21): 9685-9689.
12. Tan X, Yao L, Gao Q, Wang W, Qi F, Lu X (2011) Photosynthesis driven conversion of carbon dioxide to fatty alcohols and hydrocarbons in cyanobacteria. Metab Eng 13(2): 169-176.
13. Tan X, Liang F, Cai K, Lu X (2013) Application of the FLP/FRT recombination system in cyanobacteria for construction of markerless mutants. Appl Microbiol Biotechnol. doi: 10. 1007/s00253-013-4837-6.
14. 2 to ethylene in recombinant cyanobacterium Synechocystis 6803. Energy Environ Sci 5(10): 8998.
15. Williams JGK (1988) Construction of specific mutations in photosystem II photosynthetic reaction center by genetic engineering methods in Synechocystis 6803. Methods Enzymol 167: 766-778.