Application of the FLP/FRT recombination system in cyanobacteria for construction of markerless mutants

Applied Microbiology and Biotechnology

Volumn 97, Issue 14, 2013, Pages 6373-6382

  Tan, Xiaoming ^a   Liang, Feiyan ^a,b   Cai, Ke ^a   Lu, Xuefeng ^a  

^a QINGDAO INSTITUTE OF BIOENERGY AND BIOPROCESS TECHNOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Biofuels; Cyanobacteria; FLP FRT; Genetic engineering; Marker recycling; Markerless mutant

Indexed keywords

CYANOBACTERIA; FLP/FRT; MARKER RECYCLING; MARKERLESS; MICROBIAL CELL FACTORIES; REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION; SYNECHOCOCCUS ELONGATUS; TRANSCRIPTIONAL ANALYSIS;

BIOFUELS; DNA SEQUENCES; GENES; GENETIC ENGINEERING; RECYCLING; YEAST;

TRANSCRIPTION;

BACTERIAL VECTOR; RECOMBINASE;

CYANOBACTERIUM; DILUTION; DNA; GENETIC ENGINEERING; GENETIC MARKER; GENOTYPE; GROWTH RATE; MUTAGENICITY; POLYMERASE CHAIN REACTION; YEAST;

ARTICLE; BACTERIAL GENOME; BACTERIUM MUTANT; CONTROLLED STUDY; DNA MODIFICATION; DNA SEQUENCE; FLIPASE GENE; FLIPASE RECOMBINATION TARGET; FUNGAL GENE; GENE CASSETTE; GENE LOCUS; GENE STRUCTURE; GENETIC RECOMBINATION; GENETIC TRANSCRIPTION; GENOTYPE; HOMOZYGOTE; IN VIVO STUDY; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SYNECHOCOCCUS ELONGATUS; SYNECHOCYSTIS;

DNA NUCLEOTIDYLTRANSFERASES; GENETIC VECTORS; MUTAGENESIS, INSERTIONAL; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; SYNECHOCOCCUS; SYNECHOCYSTIS; TRANSFORMATION, BACTERIAL;

CYANOBACTERIA; SACCHAROMYCES CEREVISIAE; SYNECHOCOCCUS ELONGATUS; SYNECHOCYSTIS SP.;

EID: 84879837089     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-4837-6     Document Type: Article

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