Identification of endogenous reference genes for RT-qPCR analysis of plasma microRNAs levels in rats with acetaminophen-induced hepatotoxicity

Journal of Applied Toxicology

Volumn 33, Issue 11, 2013, Pages 1330-1336

  Wang, Yan ^a,b   Tang, Naping ^a   Hui, Taotao ^a   Wang, Shuyan ^a,b   Zeng, Xiancheng ^a   Li, Hua ^a   Ma, Jing ^a,b  

^a National Shanghai Center for New Drug Safety Evaluation and Research   (China)

^b China State Institute of Pharmaceutical Industry   (China)

Author keywords

Acetaminophen; Circulating miRNA; Drug induced liver injury; Endogenous controls; Reference genes; RT qPCR

Indexed keywords

ALANINE AMINOTRANSFERASE; ASPARTATE AMINOTRANSFERASE; MICRORNA; MICRORNA 103; MICRORNA 122; MICRORNA 16; PARACETAMOL; RNA 5S; SMALL NUCLEAR RNA; UNCLASSIFIED DRUG; BIOLOGICAL MARKER;

ANIMAL EXPERIMENT; ANIMAL MODEL; APOPTOSIS; ARTICLE; CELL PROLIFERATION; CONTROLLED STUDY; GENE CONTROL; GENE EXPRESSION; HOUSEKEEPING GENE; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN SYNTHESIS; RAT; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SPLICING; TOXIC HEPATITIS; ANIMAL; BLOOD; GENETICS; PATHOLOGY; PROCEDURES; REPRODUCIBILITY; SPRAGUE DAWLEY RAT; STATISTICAL ANALYSIS;

RATTUS;

ACETAMINOPHEN; ANIMALS; BIOLOGICAL MARKERS; DATA INTERPRETATION, STATISTICAL; DRUG-INDUCED LIVER INJURY; MALE; MICRORNAS; RATS, SPRAGUE-DAWLEY; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS;

EID: 84884700735     PISSN: 0260437X     EISSN: 10991263     Source Type: Journal    
DOI: 10.1002/jat.2864     Document Type: Article

References (29)

1. Adachi T, Nakanishi M, Otsuka Y, Nishimura K, Hirokawa G, Goto Y, Nonoqi H, Iwai N. 2010. Plasma microRNA 499 as a biomarker of acute myocardial infarction. Clin. Chem. 56: 1183-1185.
2. Andersen CL, Jensen JL, Orntoft TF. 2004. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 64: 5245-5250.
3. Arroyo JD, Chevillet JR, Kroh EM, Ruf IK, Pritchard CC, Gibson DF, Mitchell PS, Bennett CF, Pogosova-Agadjanyan EL, Stirewalt DL, Tait JF, Tewari M. 2011. Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma. Proc. Natl. Acad. Sci. U. S. A. 108: 5003-5008.
4. Brase JC, Wuttig D, Kuner R, Sultmann H. 2010. Serum microRNAs as non-invasive biomarkers for cancer. Mol. Cancer 26: 306.
5. Bustin SA, Benes V, Nolan T, Pfaffl MW. 2005. Quantitative real-time RT-PCR: a perspective. J. Mol. Endocrinol. 34: 597-601.
6. Davoren PA, McNeill RE, Lowery AJ, Kerin MJ, Miller N. 2008. Identification of suitable endogenous control genes for microRNA gene expression analysis in human breast cancer. BMC Mol. Biol. 9: 76.
7. Fox BC, Devonshire AS, Schutte ME, Foy CA, Minguez J, Przyborski S, Maltman D, Bokhari M, Marshall D. 2010. Validation of reference gene stability for APAP hepatotoxicity studies in different in vitro systems and identification of novel potential toxicity biomarkers. Toxicol. In Vitro 24: 1962-1970.
8. Guenin S, Mauriat M, Pelloux J, Van Wuytswinkel O, Bellini C, Gutierrez L. 2009. Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of references. J. Exp. Bot. 60: 487-493.
9. Hunter MP, Ismail N, Zhang X, Aguda BD, Lee EJ, Yu L, Xiao T, Schafer J, Lee ML, Schmittgen TD, Nana-Sinkam SP, Jarjoura D, Marsh CB. 2008. Detection of microRNA expression in human peripheral blood microvesicles. PLoS One 3: e3694.
10. Kang K, Peng X, Luo J, Gou D. 2012. Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling. J. Anim. Sci. Biotechnol. 3: 4.
11. Lardizábal MN, Nocito AL, Daniele SM, Ornella LA, Palatnik JF, Veggi LM. 2012. Reference genes for real-time PCR quantification of microRNAs and messenger RNAs in rat models of hepatotoxicity. PLoS One 7: e36323.
12. Laterza OF, Lim L, Garrett-Engele PW, Viasakova K, Muniappa N, Tanaka WK, Johnson JM, Sina JF, Fare TL, Sistare FD, Glaab WE. 2009. Plasma microRNAs as sensitive and specific biomarkers of tissue injury. Clin. Chem. 55: 1977-1983.
13. Lim QE, Zhou L, Ho YK, Wan G, Too HP. 2011. snoU6 and 5S RNAs are not reliable miRNA reference genes in neuronal differentiation. Neuroscience 199: 32-43.
14. Mitchell PS, Parkin RK, Kroh EM, Fritz BR, Wyman SK, Pogosova-Agadjanyan EL, Peterson A, Noteboom J, O'Briant KC, Allen A, Lin DW, Urban N, Drescher CW, Knudsen BS, Stirewalt D, Gentleman R, Vessella RL, Nelson PS, Martin DB, Tewari M. 2008. Circulating microRNAs as stable blood-based markers for cancer detection. Proc. Natl. Acad. Sci. U. S. A. 105: 10513-10518.
15. Peltier HJ, Latham GJ. 2008. Normalization of microRNA expression levels in quantitative RT-PCR assays: identification of suitable reference RNA targets in normal and cancerous human solid tissues. RNA 14: 844-852.
16. Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP. 2004. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations. Biotechnol. Lett. 26: 509-515.
17. Schilling A, Corey R, Leonard M, Eqhtesad B. 2010. Acetaminophen: old drug, new warnings. Cleve. Clin. J. Med. 77: 19-27.
18. Silver N, Best S, Jiang J, Thein SL. 2006. Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol. Biol. 7: 33.
19. Song J, Bai Z, Han W, Zhang J, Meng H, Bi J, Ma X, Han S, Zhang Z. 2012. Identification of suitable reference genes for qPCR analysis of serum microRNA in gastric cancer patients. Dig. Dis. Sci. 57: 897-904.
20. Starkey Lewis PJ, Dear J, Platt V, Simpson KJ, Craig DGN, Antoine DJ, French NS, Dhaun N, Webb DJ, Costello EM, Neoptolemos JP, Moggs J, Goldring CE, Park K. 2011. Circulating microRNAs as potential markers of human drug-induced liver injury. Hepatology 54: 1767-1776.
21. Stirnimann G, Kessebohm K, Lauterburg B. 2010. Liver injury caused by drugs: an update. Swiss Med. Wkly. 140: w13080.
22. Taylor DD, Gercel-Taylor C. 2008. microRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer. Gynecol. Oncol. 110: 13-21.
23. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. 2002. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3: 0034.1-0034.11.
24. Wang K, Zhang S, Marzolf B, Troisch P, Brightman A, Hu ZY, Hood LE, Galas DJ. 2009. Circulating microRNAs, potential biomarkers for drug-induced liver injury. Proc. Natl. Acad. Sci. U. S. A. 106: 4402-4407.
25. Wang K, Zhang S, Weber J, Baxter D, Galas DJ. 2010. Export of microRNAs and microRNA-protective protein by mammalian cells. Nucleic Acids Res. 38: 7248-7259.
26. Watkins PB. 2011. Drug safety sciences and the bottleneck in drug development. Clin. Pharmacol. Ther. 89: 788-790.
27. Yang X, Greenhaw J, Shi Q, Su Z, Qian F, Davis K, Mendrick DL, Salminen WF. 2012. Identification of urinary micrornas profiles in rats that may diagnose hepatotoxicity. Toxicol. Sci. 125: 335-344.
28. Zhang Y, Jia Y, Zheng RY, Guo YJ, Wang Y, Guo H, Fei MY, Sun SH. 2010. Plasma microRNA-122 as a biomarker for viral-, alcohol-, and chemical- related hepatic diseases. Clin. Chem. 56: 1830-1838.
29. Zhu HT, Dong QZ, Wang G, Zhou HJ, Ren N, Jia HL, Ye QH, Qin LX. 2012. Identification of suitable reference genes for qRT-PCR analysis of circulating microRNAs in hepatitis B virus-infected patients. Mol. Biotechnol. 50: 49-56.