Strategies to construct null and conditional null Trypanosoma brucei mutants using Cre-recombinase and loxP

Molecular and Biochemical Parasitology

Volumn 191, Issue 1, 2013, Pages 16-19

  Kim, Hee Sook ^a   Li, Zhen ^a   Boothroyd, Catharine ^a   Cross, George A M ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

Conditional gene knockout; Cre recombinase; Gene knockout; Trypanosoma brucei

Indexed keywords

CRE RECOMBINASE;

ALLELE; ARTICLE; CELL VIABILITY; GENE DELETION; GENE LOCUS; GENE SILENCING; KNOCKOUT GENE; LOXP SITE; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PHENOTYPE; PRIORITY JOURNAL; PROTEIN RNA BINDING; TRYPANOSOMA BRUCEI;

ALD; ALDOLASE; BLASTICIDIN-RESISTANCE GENE; BLE; BLEOMYCIN-RESISTANCE GENE; BSD; CONDITIONAL GENE KNOCKOUT; CRE-RECOMBINASE; GANCYCLOVIR; GCV; GENE KNOCKOUT; HERPES SIMPLEX VIRUS THYMIDINE KINASE; HSVTK OR TK; HYG; HYGROMYCIN-RESISTANCE GENE; KNOCKOUT; KO; NEO; NEOMYCIN-RESISTANCE GENE; OPEN READING FRAME; ORF; PUR; PUROMYCIN-RESISTANCE GENE; SAS; SPLICE ACCEPTER SITE; TRYPANOSOMA BRUCEI; TUB; TUBULIN; UNTRANSLATED REGION; UTR;

GENE KNOCKOUT TECHNIQUES; GENES, ESSENTIAL; GENETIC VECTORS; GENETICS, MICROBIAL; INTEGRASES; MOLECULAR BIOLOGY; PARASITOLOGY; SELECTION, GENETIC; TRYPANOSOMA BRUCEI BRUCEI;

EID: 84884648347     PISSN: 01666851     EISSN: 18729428     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2013.08.001     Document Type: Article

References (11)

1. L. Peacock, V. Ferris, R. Sharma, J. Sunter, M. Bailey, M. Carrington, and W. Gibson Identification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse fly Proceedings of the National Academy of Sciences of the United States of America 108 2011 3671 3676
2. H.S. Kim, S.H. Park, and A. Günzl Cross GAM MCM-BP is required for repression of life-cycle specific genes transcribed by RNA polymerase I in the mammalian infectious form of Trypanosoma brucei PLoS ONE 8 2013 e57001
3. T.N. Siegel, K.S. Tan, and G.A.M. Cross A systematic study of sequence motifs for RNA trans splicing in Trypanosoma brucei Molecular and Cellular Biology 25 2005 9586 9594 (Pubitemid 41507857)
4. M.D. Scahill, I. Pastar, and G.A.M. Cross CRE recombinase-based positive-negative selection systems for genetic manipulation in Trypanosoma brucei Molecular and Biochemical Parasitology 157 2008 73 82 (Pubitemid 350160976)
5. R.L. Barnes, and R. McCulloch Trypanosoma brucei homologous recombination is dependent on substrate length and homology, though displays a differential dependence on mismatch repair as substrate length decreases Nucleic Acids Research 35 2007 3478 3493 (Pubitemid 47073608)
6. I. Benmerzouga, J. Concepcion-Acevedo, H.S. Kim, A.V. Vandoros, G.A.M. Cross, M.M. Klingbeil, and B. Li Trypanosoma brucei Orc1 is essential for nuclear DNA replication and affects both VSG silencing and VSG switching Molecular Microbiology 87 2013 196 210
7. H.S. Kim, and G.A.M. Cross Identification of Trypanosoma brucei RMI1/BLAP75 homologue and its roles in antigenic variation PLoS ONE 6 2011 e25313
8. H.S. Kim, and G.A.M. Cross TOPO3alpha influences antigenic variation by monitoring expression-site-associated VSG switching in Trypanosoma brucei PLoS Pathogens 6 2010 e1000992
9. E. Wirtz, S. Leal, C. Ochatt, and G.A.M. Cross A tightly regulated inducible expression system for dominant negative approaches in Trypanosoma brucei Molecular and Biochemical Parasitology 99 1999 89 101 (Pubitemid 29127189)
10. K.G. Milne, M.L. Guther, and M.A.J. Ferguson Acyl-CoA binding protein is essential in bloodstream form Trypanosoma brucei Molecular and Biochemical Parasitology 112 2001 301 304 (Pubitemid 32167108)
11. M. Oberholzer, S. Morand, S. Kunz, and T. Seebeck A vector series for rapid PCR-mediated C-terminal in situ tagging of Trypanosoma brucei genes Molecular and Biochemical Parasitology 145 2006 117 120 (Pubitemid 41691861)