Cytokine-induced changes in the gene expression profile of a human cerebral microvascular endothelial cell-line, hCMEC/D3

Fluids and Barriers of the CNS

Volumn 10, Issue 1, 2013, Pages

  Lopez Ramirez, Miguel A ^a,c   Male, David K ^a   Wang, Chunfang ^a   Sharrack, Basil ^b   Wu, Dongsheng ^a   Romero, Ignacio A ^a  

^a OPEN UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF SHEFFIELD   (United Kingdom)

^c YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Blood brain barrier; Cytokines; Integrin focal adhesions; Inter brain endothelial junctions; Transcriptome; Transporter systems

Indexed keywords

BETA1 INTEGRIN; BREAST CANCER RESISTANCE PROTEIN; CARCINOEMBRYONIC ANTIGEN RELATED CELL ADHESION MOLECULE 1; CLAUDIN 5; CYTOKINE; GAMMA INTERFERON; GLUCOSE TRANSPORTER 1; GLUCOSE TRANSPORTER 3; MESSENGER RNA; MULTIDRUG RESISTANCE PROTEIN; MULTIDRUG RESISTANCE PROTEIN 1; TUMOR NECROSIS FACTOR ALPHA; VASCULAR CELL ADHESION MOLECULE 1;

ARTICLE; BLOOD BRAIN BARRIER; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMAN; HUMAN CELL; IN VITRO STUDY; MICROARRAY ANALYSIS; NERVOUS SYSTEM INFLAMMATION; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; VASCULAR ENDOTHELIUM;

EID: 84884535611     PISSN: None     EISSN: 20458118     Source Type: Journal    
DOI: 10.1186/2045-8118-10-27     Document Type: Article

References (25)

1. Coureuil M, Mikaty G, Miller F, Lecuyer H, Bernard C, Bourdoulous S, Dumenil G, Mege RM, Weksler BB, Romero IA, Couraud PO, Nassif X. Meningococcal type IV pili recruit the polarity complex to cross the brain endothelium. Science 2009, 325:83-87. 10.1126/science.1173196, 19520910.
2. Fletcher NF, Wilson GK, Murray J, Hu K, Lewis A, Reynolds GM, Stamataki Z, Meredith LW, Rowe IA, Luo G, Lopez-Ramirez MA, Baumert TF, Weksler B, Couraud PO, Kim KS, Romero IA, Jopling C, Morgello S, Balfe P, McKeating JA. Hepatitis C virus infects the endothelial cells of the blood-brain barrier. Gastroenterology 2012, 142:634-643. 10.1053/j.gastro.2011.11.028, 22138189.
3. Bahbouhi B, Berthelot L, Pettre S, Michel L, Wiertlewski S, Weksler B, Romero IA, Miller F, Couraud PO, Brouard S, Laplaud DA, Soulillou JP. Peripheral blood CD4+ T lymphocytes from multiple sclerosis patients are characterized by higher PSGL-1 expression and transmigration capacity across a human blood-brain barrier-derived endothelial cell line. J Leukoc Biol 2009, 86:1049-1063. 10.1189/jlb.1008666, 19696154.
4. Poller B, Drewe J, Krahenbuhl S, Huwyler J, Gutmann H. Regulation of BCRP (ABCG2) and P-glycoprotein (ABCB1) by cytokines in a model of the human blood-brain barrier. Cell Mol Neurobiol 2010, 30:63-70. 10.1007/s10571-009-9431-1, 19629677.
5. Tai LM, Reddy PS, Lopez-Ramirez MA, Davies HA, Male DK, Loughlin AJ, Romero IA. Polarized P-glycoprotein expression by the immortalised human brain endothelial cell line, hCMEC/D3, restricts apical-to-basolateral permeability to rhodamine 123. Brain Res 2009, 1292:14-24.
6. Schreibelt G, Kooij G, Reijerkerk A, van Doorn R, Gringhuis SI, van der Pol S, Weksler BB, Romero IA, Couraud PO, Piontek J, Blasig IE, Dijkstra CD, Ronken E, de Vries HE. Reactive oxygen species alter brain endothelial tight junction dynamics via RhoA, PI3 kinase, and PKB signaling. FASEB J 2007, 21:3666-3676. 10.1096/fj.07-8329com, 17586731.
7. Afonso PV, Ozden S, Prevost MC, Schmitt C, Seilhean D, Weksler B, Couraud PO, Gessain A, Romero IA, Ceccaldi PE. Human blood-brain barrier disruption by retroviral-Infected lymphocytes: role of myosin light chain kinase in endothelial tight-junction disorganization. J Immunol 2007, 179:2576-2583.
8. Lopez-Ramirez MA, Fischer R, Torres-Badillo CC, Davies HA, Logan K, Pfizenmaier K, Male DK, Sharrack B, Romero IA. Role of caspases in cytokine-induced barrier breakdown in human brain endothelial cells. J Immunol 2012, 189:3130-3139. 10.4049/jimmunol.1103460, 22896632.
9. Forster C, Burek M, Romero IA, Weksler B, Couraud PO, Drenckhahn D. Differential effects of hydrocortisone and TNF{alpha} on tight junction proteins in an in vitro model of the human blood-brain barrier. J Physiol 2008, 586:1937-1949. 10.1113/jphysiol.2007.146852, 2375735, 18258663.
10. Huang DW, Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protocols 2008, 4:44-57.
11. Viemann D, Goebeler M, Schmid S, Klimmek K, Sorg C, Ludwig S, Roth J. Transcriptional profiling of IKK2/NF-kappa B- and p38 MAP kinase-dependent gene expression in TNF-alpha-stimulated primary human endothelial cells. Blood 2004, 103:3365-3373. 10.1182/blood-2003-09-3296, 14715628.
12. Franzen B, Duvefelt K, Jonsson C, Engelhardt B, Ottervald J, Wickman M, Yang Y, Schuppe-Koistinen I. Gene and protein expression profiling of human cerebral endothelial cells activated with tumor necrosis factor-alpha. Brain Res Mol Brain Res 2003, 115:130-146. 10.1016/S0169-328X(03)00185-2, 12877984.
13. Kraft P, Benz PM, Austinat M, Brede ME, Schuh K, Walter U, Stoll G, Kleinschnitz C. deficiency of vasodilator-stimulated phosphoprotein (VASP) increases blood-brain-barrier damage and edema formation after ischemic stroke in mice. PloS one 2010, 5:e15106. 10.1371/journal.pone.0015106, 2997079, 21151938.
14. Viemann D, Goebeler M, Schmid S, Nordhues U, Klimmek K, Sorg C, Roth J. TNF induces distinct gene expression programs in microvascular and macrovascular human endothelial cells. J Leukoc Biol 2006, 80:174-185. 10.1189/jlb.0905530, 16617158.
15. Nouvion AL, Oubaha M, Leblanc S, Davis EC, Jastrow H, Kammerer R, Breton V, Turbide C, Ergun S, Gratton JP, Beauchemin N. CEACAM1: a key regulator of vascular permeability. J Cell Sci 2010, 123:4221-4230. 10.1242/jcs.073635, 21081647.
16. Reijerkerk A, Lopez-Ramirez MA, van het Hof B, Joost AR, Drexhage WK, Kooij G, Joost BV, van der Pouw Kraan TCTM, Anton J, Van Z, Horrevoets AJ, Prat A, Romero IA, de Vries HE. microRNAs regulate human brain endothelial cell barrier function in inflammation: implications for multiple sclerosis. J Neurosci 2013, 16:6857-6863.
17. Lopez-Ramirez MA. Investigating the role of inflammation-modulated microRNAs and signalling pathways in blood-brain barrier dysfunction. PhD Thesis 2012, The Open University, Department of Life, Health and Chemical Sciences.
18. McKenzie J, Ridley A. Roles of Rho/ROCK and MLCK in TNF-alpha-induced changes in endothelial morphology and permeability. J Cell Physiol 2007, 213:221-228. 10.1002/jcp.21114, 17476691.
19. Daneman R, Zhou L, Kebede AA, Barres BA. Pericytes are required for blood-brain barrier integrity during embryogenesis. Nature 2010, 468:562-566. 10.1038/nature09513, 3241506, 20944625.
20. Alexander JS, Elrod JW. Extracellular matrix, junctional integrity and matrix metalloproteinase interactions in endothelial permeability regulation. J Anat 2002, 200:561-574. 10.1046/j.1469-7580.2002.00057.x, 1570742, 12162724.
21. Schlegel N, Waschke J. Impaired integrin-mediated adhesion contributes to reduced barrier properties in VASP-deficient microvascular endothelium. J Cell Physiol 2009, 220:357-366. 10.1002/jcp.21772, 19347869.
22. Osada T, Gu YH, Kanazawa M, Tsubota Y, Hawkins BT, Spatz M, Milner R, Del Zoppo GJ. Interendothelial claudin-5 expression depends on cerebral endothelial cell-matrix adhesion by beta(1)-integrins. J Cereb Blood Flow Metab 2001, 31:1972-1985.
23. Urich E, Lazic SE, Molnos J, Wells I, Freskgard PO. Transcriptional profiling of human brain endothelial cells reveals key properties crucial for predictive in vitro blood-brain barrier models. PloS One 2012, 7:e38149. 10.1371/journal.pone.0038149, 3364980, 22675443.
24. Subileau E, Rezaie P, Davies H, Colyer F, Greenwood J, Male D, Romero I. Expression of chemokines and their receptors by human brain endothelium: implications for multiple sclerosis. J Neuropathol Exp Neurol 2009, 68:227-240. 10.1097/NEN.0b013e318197eca7, 19225413.
25. Pan W, Yu C, Hsuchou H, Khan RS, Kastin AJ. Cerebral microvascular IL15 is a novel mediator of TNF action. J Neurochem 2009, 111:819-827. 10.1111/j.1471-4159.2009.06371.x, 2785453, 19719822.