Reduction of autofluorescence at the microelectrode-cortical tissue interface improves antibody detection

Journal of Neuroscience Methods

Volumn 203, Issue 1, 2012, Pages 96-105

  Potter, Kelsey A ^a,b   Simon, Joel S ^a,b   Velagapudi, Bharath ^a,b   Capadona, Jeffrey R ^a,b  

^a Case Western Reserve University   (United States)

^b LOUIS STOKES CLEVELAND VA MEDICAL CENTER   (United States)

Author keywords

Autofluorescence; Copper sulfate; Hemosiderin laden macrophage; Immunohistochemistry; Neural electrode

Indexed keywords

COPPER SULFATE; GLIAL FIBRILLARY ACIDIC PROTEIN; GREEN FLUORESCENT PROTEIN; HEMOSIDERIN; IMMUNOGLOBULIN G; RED FLUORESCENT PROTEIN;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIBODY LABELING; ANTIGEN DETECTION; ANTIGEN EXPRESSION; ARTICLE; AUTOFLUORESCENCE; BRAIN CORTEX; BRAIN TISSUE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; FLUORESCENCE ANALYSIS; IMMERSION; IMMUNOHISTOCHEMISTRY; MACROPHAGE; MALE; MICROELECTRODE; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; CEREBRAL CORTEX; COPPER SULFATE; ELECTRODES, IMPLANTED; HEMOSIDERIN; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOHISTOCHEMISTRY; INFLAMMATION; MACROPHAGES; MALE; MICROELECTRODES; MICROSCOPY, FLUORESCENCE; RATS; RATS, SPRAGUE-DAWLEY; SIGNAL-TO-NOISE RATIO;

EID: 81555228640     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2011.09.024     Document Type: Article

References (48)

1. Abbott N.J., Rönnbäck L., Hansson E. Astrocyte-endothelial interactions at the blood-brain barrier. Nat. Rev. Neurosci. 2006, 7:41-53.
2. Aihara N., Tanno H., Hall J.J., Pitts L.H., Noble L.J. Immunocytochemical localization of immunoglobulins in the rat brain: relationship to the blood-brain barrier. J. Comp. Neurol. 1994, 342:481-496.
3. Azemi E., Lagenaur C.F., Cui X.T. The surface immobilization of the neural adhesion molecule L1 on neural probes and its effect on neuronal density and gliosis at the probe/tissue interface. Biomaterials 2011, 32:681-692.
4. Barden H. Further histochemical studies characterizing the lipofuscin component of human neuromelanin. J. Neuropathol. Exp. Neurol. 1978, 37:437-451.
5. Biran R., Martin D., Tresco P. Neuronal cell loss accompanies the brain tissue response to chronically implanted silicon microelectrode arrays. Exp. Neurol. 2005, 195:115-126.
6. Bjornsson C.S., Oh S.J., Al-Kofahi Y.A., Lim Y.J., Smith K.L., Turner J.N., De S., Roysam B., Shain W., Kim S.J. Effects of insertion conditions on tissue strain and vascular damage during neuroprosthetic device insertion. J. Neural Eng. 2006, 3(3):196-207.
7. Brunk U.T., Terman A. The mitochondrial-lysosomal axis theory of aging. Accumulation of damaged mitochondria as a result of imperfect autophagocytosis. Eur. J. Biochem. 2002, 269:1996-2002.
8. Clancy B., Cauller L.J. Reduction of background autofluorescence in brain sections following immersion in sodium borohydride. J. Neurosci. Methods 1998, 83:97-102.
9. Cook S.F. The structure and composition of hemosiderin. J. Biol. Chem. 1929, 82:595-609.
10. Craig A.S. Sodium borohydride as an aldehyde blocking reagent for electron microscope histochemistry. Histochemistry 1974, 42:141-144.
11. Csallany A.S., Ayaz K.L. Quantitative determination of organic solvent soluble lipofuscin pigments in tissues. Lipids 1975, 11:412-417.
12. Fitch M.T., Silver J. CNS injury, glial scars, and inflammation: Inhibitory extracellular matrices and regeneration failure. Exp. Neurol. 2008, 209:294-301.
13. Frampton J.P., Hynd M.R., Shuler M.L., Shain W. Effects of glial cells on electrode impedance recorded from neural prosthetic devices in vitro. Ann. Biomed. Eng. 2010, 38:1031-1047.
14. Goldfischer S., Bernstein J. Lipofuscin (aging) pigment granules of the newborn human liver. J. Cell Biol. 1969, 42:253-261.
15. Harris JP, Capadona JR, Miller RH, Healy BC, Shanmuganathan K, Rowan SJ, Weder C, Tyler DJ. Mechanically adaptive intracortical implants improve the proximity of neuronal cell bodies. J Neural Eng 2011a, in press.
16. Harris J.P., Hess A.E., Rowan S.J., Weder C., Zorman C.A., Tyler D.J., Capadona J.R. In vivo deployment of mechanically adaptive nanocomposites for intracortical microelectrodes. J. Neural Eng. 2011, 8:046010.
17. He W., Bellamkonda R.V. Molecular perspective on understanding and modulating the performance of chronic central nervous system (CNS) recording electrodes. Indwelling Neural Implants: Strategies for Contending with the In Vivo Environment 2008, CRC Press. W. Reichert (Ed.).
18. He W., McConnell G.C., Schneider T.M., Bellamkonda R.V. A novel anti-inflammatory surface for neural electrodes. Adv. Mater. 2007, 19:3529-3533.
19. Höhn A., Jung T., Grimm S., Grune T. Lipofuscin-bound iron is a major intracellular source of oxidants: role in senescent cells. Free Radic. Biol. Med. 2010, 48:1100-1108.
20. Jung T., Hohn A., Grune T. Detection and quantification by microscopic techniques. Advanced Protocols in Oxidative Stress II 2010, 173-193. Humana Press. D. Armstrong (Ed.).
21. Kikugawa K., Beppu M., Sato A., Kasai H. Separation of multiple yellow fluorescent lipofuscin components in rat kidney and their characterization. Mech. Ageing Dev. 1997, 97:93-107.
22. Kim D., Smith J., Chilkoti A., Reichert W. The effect of covalently immobilized rhIL-1ra-ELP fusion protein on the inflammatory profile of LPS-stimulated human monocytes. Biomaterials 2007, 28:3369-3377.
23. Kim S.-S., Ye C., Kumar P., Chiu I., Subramanya S., Wu H., Shankar P., Manjunath N. Targeted delivery of siRNA to macrophages for anti-inflammatory treatment. Mol. Ther. 2010, 18:993-1001.
24. Leach J., Achyuta A.K.H., Murthy S.K. Bridging the divide between neuroprosthetic design, tissue engineering and neurobiology. Frontiers in Neuroengineering 2010, 2.
25. Leung B.K., Biran R., Underwood C.J., Tresco P.A. Characterization of microglial attachment and cytokine release on biomaterials of differing surface chemistry. Biomaterials 2008, 29:3289-3297.
26. Li L., Lu J., Tay S., Moochhala S., He B. The function of microglia, either neuroprotection or neurotoxicity, is determined by the equilibrium among factors released from activated microglia in vitro. Brain Res. 2007, 1159:8-17.
27. Liem R.K.H., Messing A. Dysfunctions of neuronal and glial intermediate filaments in disease. J. Clin. Invest. 2009, 119:1814-1824.
28. Liu C., Shi Z., Fan L., Zhang C., Wang K., Wang B. Resveratrol improves neuron protection and functional recovery in rat model of spinal cord injury. Brain Res. 2011, 1374:100-109.
29. McConnell G.C., Rees H.D., Levey A.I., Gutekunst C.-A., Gross R.E., Bellamkonda R.V. Implanted neural electrodes cause chronic, local inflammation that is correlated with local neurodegeneration. Journal of Neural Engineering 2009, 6:056003.
30. O'Connell M.J., Baum H., Peters T.J. Haemosiderin-like properties of free-radical-modified ferritin. Biochemistry Journal 1986, 240:297-300.
31. Polikov V., Tresco P., Reichert W. Response of brain tissue to chronically implanted neural electrodes. J. Neurosci. Methods 2005, 148:1-18.
32. Polikov V.S., Block M.L., Fellous J.-M., Hong J.-S., Reichert W.M. In vitro model of glial scarring around neuroelectrodes chronically implanted in the CNS. Biomaterials 2006, 27:5368-5376.
33. Polikov V.S., Hong J.S., Reichert W.M. Soluble factor effects on glial cell reactivity at the surface of gel-coated microwires. J. Neurosci. Methods 2010, 190:180-187.
34. Reichel W., Bollander J., Clark J.H., Strehler B.L. Lipofuscin pigment accumulation as a function of age and distribution in rodent brain. J. Gerontol. 1968, 23:71-78.
35. Rich A.R. The formation of bile pigment. Physiology Reviews 1925, 5:182-224.
36. Romijn H.J., van Uum J.F.M., Breedijk I., Emmering J., Radu I., Pool C.W. Double immunolabeling of neuropeptides in the human hypothalamus as analyzed by confocal laser scanning fluorescence microscopy. J. Histochem. Cytochem. 1999, 47:229-235.
37. Schnell S.A., Staines W.A., Wessendorf M.W. Reduction of lipofuscin-like autofluorescence in fluorescently labeled tissue. J. Histochem. Cytochem. 1999, 47:719-730.
38. Schwartz A.B. Cortical neural prosthetics. Annu. Rev. Neurosci. 2004, 27:487-507.
39. Skousen J., Merriam S., Srivannavit O., Perlin G., Wise K., Tresco P. Reducing surface area while maintaining implant penetrating profile lowers the brain foreign body response to chronically implanted planar silicon microelectrode arrays. Prog. Brain Res. 2011, 194C:167-180.
40. Spanswick S.C., Bray D., Zelinski E.L., Sutherland R.J. A novel method for reliable nuclear antibody detection in tissue with high levels of pathology-induced autofluorescence. J. Neurosci. Methods 2009, 185:45-49.
41. Spitzer N., Sammons G.S., Price E.M. Autofluorescent cells in rat brain can be convincing impostors in green fluorescent reporter studies. J. Neurosci. Methods 2011, 197(1):48-55.
42. Steiner R.F., Kirby E.P. The interaction of the ground and excited states of indole derivatives with electron scavengers. J. Phys. Chem. 1969, 73:4130-4135.
43. Szarowski D. Brain responses to micro-machined silicon devices. Brain Res. 2003, 983:23-35.
44. Tresco P.A., Winslow B.D. The challenge of integrating devices into the central nervous system. Crit. Rev. Biomed. Eng. 2011, 39:29-44.
45. Turner J.N., Shain W., Szarowski D.H., Andersen M., Martins S., Isaacson M., Craighead H. Cerebral astrocyte response to micromachined silicon implants. Exp. Neurol. 1999, 156:33-49.
46. Ward M.P., Rajdev P., Ellison C., Irazoqui P.P. Toward a comparison of microelectrodes for acute and chronic recordings. Brain Res. 2009, 1282:183-200.
47. Weir M.P., Gibson J.F., Peters T.J. Biochemical studies on the isolation and characterization of human spleen haemosiderin. Biochem. J 1984, 223:31-38.
48. Winslow B.D., Christensen M.B., Yang W.-K., Solzbacher F., Tresco P.A. A comparison of the tissue response to chronically implanted parylene-C-coated and uncoated planar silicon microelectrode arrays in rat cortex. Biomaterials 2010, 31:9163-9172.