Sudan Black B treatment reduces autofluorescence and improves resolution of in situhybridization specific fluorescent signals of brain sections

Histology and Histopathology

Volumn 25, Issue 8, 2010, Pages 1017-1024

  Oliveira, V C ^a   Carrara, R C V ^a   Simoes, D L C ^a   Saggioro, F P ^a   Carlotti, C G ^a   Covas, D T ^a   Neder, L ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Autofluorescence; Fluorescence microscopy; Human brain; Paraffin sections; Sudan Black B

Indexed keywords

AMMONIA; AZO COMPOUND; COLORING AGENT; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; FORMALDEHYDE; PARAFFIN; SUDAN BLACK;

ARTICLE; BLEACHING; BRAIN; COMPARATIVE STUDY; FLUORESCENCE; FLUORESCENT ANTIBODY TECHNIQUE; FROZEN SECTION; HISTOLOGY; HUMAN; METHODOLOGY; NUCLEIC ACID HYBRIDIZATION;

AMMONIA; AZO COMPOUNDS; BRAIN; COLORING AGENTS; FLUORESCENCE; FLUORESCENT ANTIBODY TECHNIQUE; FLUORESCENT ANTIBODY TECHNIQUE, DIRECT; FORMALDEHYDE; FROZEN SECTIONS; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; HISTOLOGICAL TECHNIQUES; HUMANS; INDICATORS AND REAGENTS; NUCLEIC ACID HYBRIDIZATION; PARAFFIN EMBEDDING; PHOTOBLEACHING;

EID: 77956918736     PISSN: 02133911     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Baschong W., Dürrenberger M., Mandinova A. and Suetterlin R. (1999). Three dimensional visualization of the cytoskeleton by confocal laser scanning microscopy. In: Confocal microscopy. Cohen P. M. (ed). Methods Enzymol. 307, 173-189.
2. Baschong W., Sütterlin R. and Aebi U. (1997). Punch-wounded, fibroblast-populated collagen matrices. A novel approach for studying cytoskeletal changes in 3-dimensions by confocal laser scanning microscopy. Eur. J. Cell Biol. 72, 189-201.
3. Baschong W., Suetterlin R. and Laeng R. H. (2001). Control of autofluorescence of archival formaldehyde-fixed, paraffin-embedded tissue in confocal laser scanning microscopy (CLSM). J. Histochem. Cytochem. 49, 1565-1571.
4. Belichenko P. V., Fedorov A. A. and Dahlstrom A. B. (1996). Quantitative analysis of immunofluorescence and lipofuscin distribution in human cortical areas by dual-channel confocal laser scanning microscopy. J. Neurosci. Methods 69, 155-161.
5. Braak H. (1976). Pigment architecture of human cerebral córtex. Verh. Anat. Ges. 70, 1003-1006.
6. Clancy B. and Cauller L. J. (1998). Reduction of background autofluorescence in brain sections following immersion in sodium borohydride. J. Neurosci. Methods 83, 97-102.
7. Correa F. M., Innis R. B., Rouot B., Pasternak G. W. and Snyder S. H. (1980). Fluorescent probes of alpha-and beta-adrenergic and opiate receptors: biochemical and histochemical evaluation. Neurosci. Lett. 16, 47-53.
8. Cowen T. and Haven A. J. (1985). Pontamine sky blue: a counterstain for background autofluorescence in fluorescence and immunofluorescence histochemistry. Histochemistry 82, 205-208.
9. Double K. L., Dedov V. N., Fedorow H., Kettle E., Halliday G. M., Garner B. and Brunk U. T. (2008). The comparative biology of neuromelanin and lipofuscin in the human brain. Cell Mol. Life Sci. 65, 1669-1682.
10. Helen P. (1983). Fine-structural and degenerative features in adult and aged human sympathetic ganglion cells. Mech. Ageing Dev. 23, 161-175.
11. Johnson T. A. A. (1987). Glutaraldehyde fixation chemistry: oxygenconsuming reactions. Eur. J. Cell Biol. 45, 160-169.
12. Kalyuzhny A. E. and Wessendorf M. W. (1998). Relationship of mu-and delta-opioid receptors to GABAergic neurons in the central nervous system, including antinociceptive brainstem circuits. J. Comp. Neurol. 392, 528-547.
13. Mosiman V. L., Patterson B. K., Canterero L. and Goolsby C. L. (1997). Reducing cellular autofluorescence in flow cytometry: An in situ method. Cytometry. 30, 151-156.
14. Neumann M. and Gabel D. (2002). Simple Method for Reduction of autofluorescence in fluorescence microscopy. J. Histochem. Cytochem. 50, 437-439.
15. Partanen M., Santer R. M. and Hervonen A. (1980). The effect of ageing on the histochemically demonstrable catecholamines in the hypogastric (main pelvic) ganglion of the rat. Histochem. J. 12, 527-535.
16. Romijn H. J., van Uum J. F. M., Breedijk I., Emmering J., Radu I. and Pool C. W. (1999). Double immunolabeling of neuropeptides in the human hypothalamus as analyzed by confocal laser scanning fluorescence microscopy. J. Investig. Med. 47, 326-332.
17. Santer R. M., Partanen M. and Hervonen A. (1980). Glyoxylic acid fluorescence and ultrastructural studies of neurones in the coeliacsuperior mesenteric ganglion of the aged rat. Cell Tissue Res. 211, 475-485.
18. Schnell A. S., Staines W. A. and Wessendorf M. W. (1999). Reduction of lipofuscin-like autofluorescence in fluorescently labeled tissue. J. Histochem. Cytochem. 47, 719-730.
19. Southern L. J., Hughes H., Lawford P. V., Clench M. R. and Maning N. J. (2000). Glutaraldehyde-induced crosslinks: a study of model compounds and commercial bioprosthetic valves. J. Heart Valve Dis. 9, 241-248.
20. Tagliaferro P., Tandler C. J., Ramos A. J., Saavedra J. P. and Brusco A. (1997). Immunofluorescence and glutaraldehyde fixation. A new procedure based on the Schiff-quenching method. J. Neurosci. Methods 77, 191-197.
21. Terman A. and Brunk U. (2004). Lipofuscin. Int. J. Biochem. Cell Biol. 36, 1400-1404.
22. Terman A. and Brunk U. (2006). Oxidative stress, accumulation of biological "garbage" and aging. Antioxid. Redox Signal 8, 197-204.
23. Viegas M. S., Martins T. C., Seco F. and Do Carmo A. (2007). An improved and cost-effective methodology for the reduction of autofluorescence in direct immunofluorescence studies on formalinfixed paraffin-embedded tissues. Eur. J. Histochem. 51, 59-66.