Development of a strand-specific real-time qRT-PCR for the accurate detection and quantitation of West Nile virus RNA

Journal of Virological Methods

Volumn 194, Issue 1-2, 2013, Pages 146-153

  Lim, Stephanie M ^a   Koraka, Penelope ^a   Osterhaus, Albert D M E ^a   Martina, Byron E E ^a  

^a ERASMUS MEDICAL CENTER   (Netherlands)

Author keywords

Strand specific qRT PCR; Tagged qRT PCR; Virus replication kinetics; West Nile virus

Indexed keywords

COMPLEMENTARY DNA; RNA DIRECTED DNA POLYMERASE; VIRUS RNA;

ACCURACY; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BRAIN TISSUE; CONTROLLED STUDY; FEMALE; FLAVIVIRUS INFECTION; IN VITRO STUDY; IN VIVO STUDY; MICROGLIA; MOUSE; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA SYNTHESIS; SENSITIVITY AND SPECIFICITY; THERMOSTABILITY; VIRAL TROPISM; VIRUS CELL INTERACTION; VIRUS DETECTION; VIRUS LOAD; VIRUS REPLICATION; VIRUS VIRULENCE; WEST NILE FLAVIVIRUS; WEST NILE VIRUS INFECTION;

STRAND-SPECIFIC QRT-PCR; TAGGED QRT-PCR; VIRUS REPLICATION KINETICS; WEST NILE VIRUS;

ANIMALS; CELL LINE; MICE; MICE, INBRED C57BL; REAL-TIME POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; TIME FACTORS; VIRAL LOAD; WEST NILE VIRUS;

EID: 84884197759     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2013.07.050     Document Type: Article

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