Detection of infectious hepatitis A virus by integrated cell culture/strand-specific reverse transcriptase-polymerase chain reaction

Journal of Applied Microbiology

Volumn 97, Issue 5, 2004, Pages 1105-1112

  Jiang, Y J ^a   Liao, G Y ^a   Zhao, W ^a   Sun, M B ^a   Qian, Y ^a   Bian, C X ^a   Jiang, Shu De ^a  

^a INSTITUTE OF MEDICAL BIOLOGY   (China)

Author keywords

Hepatitis A virus; Infectious; Integrated cell culture stand specific reverse transcriptase polymerase chain reaction; Negative strand RNA; Replicative intermediate

Indexed keywords

ASSAYS; CELLS; CHAINS; POLYMERASE CHAIN REACTION; REACTION INTERMEDIATES; RNA; VIRUSES;

GENOMICS; HEPATITIS A VIRUS; INFECTIOUS; INTEGRATED CELL CULTURE/STAND-SPECIFIC REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION; NEGATIVE-STRAND RNA; NOVEL METHODS; RAPID METHOD; REPLICATIVE INTERMEDIATE; REVERSE TRANSCRIPTASE- POLYMERASE CHAIN REACTION; WATER SAMPLES;

CELL CULTURE;

INACTIVATED VIRUS VACCINE; NEGATIVE STRAND RNA; POSITIVE STRAND RNA; UNCLASSIFIED DRUG; VIRUS RNA;

INFECTIOUS DISEASE;

ACCURACY; ARTICLE; BIOASSAY; CELL CULTURE; CONTROLLED STUDY; HEPATITIS A VIRUS; INOCULATION; INTEGRATED CELL CULTURE STRAND SPECIFIC REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; NONHUMAN; REFERENCE VALUE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STRAND SPECIFIC REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRION; VIRUS CONCENTRATION; VIRUS DETECTION; VIRUS GENOME; VIRUS INFECTIVITY; WATER CONTAMINATION; WATER SAMPLING;

HEPATITIS A VIRUS; RNA VIRUSES;

EID: 6344269178     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2004.02413.x     Document Type: Article

References (36)

1. Beales, L.P., Wood, D.J., Minor, P.D. and Saldanha, J.A. (1996) A novel cytopathic microtitre plate assay for hepatitis A virus and antihepatitis A neutralizing antibodies. Journal of Virological Methods 59, 147-154.
2. Binn, L.N., Bancroft, W.H., Lemon, S.M., Marchwicki, R.H., LeDuc, J.W., Trahan, C.J., Staley, E.G. and Keenan, CM. (1986) Preparation of a prototype inactivated hepatitis A virus vaccine from infected cell cultures. The Journal of Infectious Diseases 153, 749-756.
3. Casas, N. and Sunen, E. (2001) Detection of enterovirus and hepatitis A virus RNA in mussels (Mytilus spp.) by reverse transcriptase-polymerase chain reaction. Journal of Applied Microbiology 90, 89-95.
4. Chaves, R.L., Graff, J., Mormann, A. and Flehmig, B. (1994) Specific detection of minus strand hepatitis A virus RNA by tail-PCR following reverse transcription. Nucleic Acids Research 22, 1919-1920.
5. Chung, H., Jaykus, L. A. and Sobsey, M.D. (1996) Detection of human enteric viruses in oysters by in vivo and in vitro amplification of nucleic acids. Applied and Environmental Microbiology 62, 3772-3778
6. Craggs, J.K., Ball, J.K., Thomson, B.J., Irving, W.L. and Grabowska, A.N. (2001) Development of a strand-specific RT-PCR based assay to detect the replicative form of hepatitis C virus RNA. Journal of Vimlogical Methods 94, 111-120.
7. Croci, L., De Medici, D., Scalfaro, C., Fiore, A. and Toti, L. (2002) The survival of hepatitis A virus in fresh produce. International Journal of Food Microbiology 73, 29-34.
8. Cromeans, T.L., Nainan, O.V. and Margolis, H.S. (1997) Detection of hepatitis A virus RNA in oyster meat. Applied and Environmental Microbiology 63, 2460-2463.
9. Cuthbert, J.A. (2001) Hepatitis A: old and new. Clinical Microbiology Reviews 14, 38-58.
10. De Chastonay, J. and Siegl, G. (1987) Replicative events in hepatitis A virus-infected MRC-5 cells. Virology 157, 268-275.
11. De Medici, D., Croci, L., Pasquale, S.D., Fiore, A. and Toti, L. (2001) Detecting the presence of infectious Hepatitis A virus in molluscs positive to RT-nested-PCR. Letters in Applied Microbiology 33, 362-366.
12. Deng, M.Y., Day, S.P. and Cliver, D.O. (1994) Detection of hepatitis A virus in environmental samples by antigen-capture RT-PCR. Applied and Environmental Microbiology 60, 1927-1933.
13. Dubois, E., Agier, C., Traore, O., Hennechart, C., Merle, G., Cruciere, C. and Veran, H.L. (2002) Modified concentration method for the detection of enteric viruses on fruits and vegetables by reverse transcriptase-polymerase chain reaction or cell culture. Journal of Food Protection 65, 1962-1969.
14. Green, D.H. and Lewis, G.D. (1999) Comparative detection of enteric viruses in wastewaters, sediments and oysters by reverse transcription-PCR and cell culture. Water Research 33, 1195-1200.
15. Halliday, M.L., Kang, L.Y., Zhou, T.K., Hu, M.D., Pan, Q.C., Fu, T.Y., Huang, S.Y. and Hu, S.L. (1991) An epidemic of hepatitis A attributable to the ingestion of raw clams in Shanghai, China. The Journal of Infectious Diseases 164, 852-859.
16. Lanford, R.E., Sureau, C., Jacob, J.R., White, R. and Fuerst, T.R. (1994) Demonstration of in vitro infection of chimpanzee hepatocytes with hepatitis C virus using strand-specific RT/PCR. Virology 202, 606-614.
17. Lemon, S.M., Binn, L.N. and Marchwicki, R.H. (1983) Radioimmunofocus assay for quantitation of hepatitis A virus in cell culture. Journal of Clinical Microbiology 17, 834-839.
18. Li, J.W., Xin, Z.T., Wang, X.W., Zheng, J.L. and Chao, F.H. (2002) Mechanisms of inactivation of hepatitis A virus by chlorine. Applied and Environmental Microbiology 68, 4951-4955.
19. Lin, L., Fevery, J. and Yap, S.H. (2002) A novel strand-specific RT-PCR for the detection of hepatitis C virus negative-strand RNA (replicative intermediate): evidence of absence or very low level of HCV replication in peripheral blood mononuclear cells. Journal of Virological Methods 100, 97-105.
20. Mao, J.S., Dong, D.X., Zhang, H.Y., Chen, N.L., Zhang, X.Y., Huang, H.Y., Xie, R.Y., Zhou, T.J. et al. (1989) Primary study of attenuated live hepatitis A vaccine (H2 strain) in humans. The Journal of Infectious Diseases 159, 621-624.
21. Mizutani, T., Ikeda, M., Saito, S., Sugiyama, K., Shimotohno, K. and Kato, N. (1998) Detection of negative-stranded hepatitis C virus RNA using a novel strand-specific reverse transcription-polymerase chain reaction. Virus Research 53, 209-214.
22. Morace, G., Aulicino, F.A., Angelozzi, C., Costanzo, L., Donadio, F. and Rapicetta, M. (2002) Microbial quality of wastewater: detection of hepatitis A virus by reverse transcriptase-polymerase chain reaction. Journal of Applied Microbiology 92, 828-836.
23. Nuanualsuwan, S. and Cliver, D.O. (2002) Pretreatment to avoid positive RT-PCR results with inactivated viruses. Journal of Virological Methods 104, 217-225.
24. Pellegrini, V., Fineschi, N., Matteucci, G., Marsili, L, Nencioni, L., Puddu, M., Garelick, H. and Zukerman, A.J. (1993) Preparation and immunogenicity of an inactivated hepatitis A vaccine. Vaccine 11, 383-387.
25. Quadri, R., Rubbia-Brandt, L., Abid, K. and Negro, F. (2001) Detection of the negative-strand hepatitis C virus RNA in tissues: implications for pathogenesis. Antiviral Research 52, 161-171.
26. Reed, L.J. and Muench, H.A. (1938) A simple method of estimating fifty percent endpoints. American Journal of Hygiene 27, 493-497.
27. Reynolds, K.A., Gerba, C.P. and Pepper, I.L. (1996) Detection of infectious enteroviruses by an integrated cell culture-PCR procedure. Applied and Environmental Microbiology 62, 1424-1427.
28. Reynolds, K.A., Gerba, C.P., Abbaszadegan, M. and Pepper, I.L. (2001) ICC/PCR detection of enteroviruses and hepatitis A virus in environmental samples. Canadian Journal of Microbilogy 47, 153-157.
29. Richards, G.P. (1999) Limitations of molecular biological techniques for assessing the virological safety of foods. Journal of Food Protection 62, 691-697.
30. Siegl, G., De Chastonay, J. and Kronauer, G. (1984) Propagation and assay of hepatitis A virus in vitro. Journal of Virological Methods 9, 53-67.
31. Taylor, M.B., Cox, N., Vrey, M.A. and Grabow, W.O. (2001) The occurrence of hepatitis A and astroviruses in selected river and dam waters in South Africa. Water Research 35, 2653-2660.
32. Weitz, M. and Siegl, G. (1993) Structure and molecular virology. In Virol hepatitis ed. Zukerman, A.J. and Howard, C.T. pp. 21-34. London: Longman Group UK limited.
33. WHO Expert Committee on Biological Standardization (1982) Requirements for poliomyelitis vaccine (inactivated). WHO Technical Report Series No. 673. Geneva, Swiss: World Health Organization.
34. Willems, M., Peerlinck, K., Moshage, H., Deleu, I., Eynde, C.V., Vermylen, J. and Yap, S.H. (1994) Hepatitis C virus-RNAs in plasma and in peripheral blood mononuclear cells of hemophiliacs with chronic hepatitis C: evidence for viral replication in peripheral blood mononuclear cells. Journal of Medical Virology 42, 272-278.
35. Xu, Z.Y., Li, Z.H., Wang, J.X., Xiao, Z.P. and Dong, D.X. (1992) Ecology and prevention of a shellfish-associated hepatitis A epidemic in Shanghai, China. Vaccine 10 (Suppl. 1), s67-s68.
36. Zachoval, R. and Deinhardt, F. (1993) Natural history and experimental models. In Viral Hepatitis, ed. Zukerman, A.J. and Howard, C.T. pp. 35-64. London, UK: Longman Group UK limited.