Fibrous star poly(ε-caprolactone) melt-electrospun scaffolds for wound healing applications

Journal of Bioactive and Compatible Polymers

Volumn 28, Issue 5, 2013, Pages 492-507

  Gazzarri, Matteo ^a   Bartoli, Cristina ^a   Mota, Carlos ^a   Puppi, Dario ^a   Dinucci, Dinuccio ^a   Volpi, Silvia ^a   Chiellini, Federica ^a  

^a UNIVERSITY OF PISA   (Italy)

Author keywords

co culture; fibroblasts; keratinocytes; Melt electrospinning; star poly( caprolactone); wound dressing

Indexed keywords

CAPROLACTONE; COCULTURE; KERATINOCYTES; MELT ELECTROSPINNING; WOUND DRESSINGS;

BIOCOMPATIBILITY; BIODEGRADABLE POLYMERS; CELL CULTURE; COLLAGEN; ELECTROSPINNING; FIBROBLASTS; SCANNING ELECTRON MICROSCOPY; STARS; TISSUE REGENERATION;

SCAFFOLDS (BIOLOGY);

COLLAGEN; POLYCAPROLACTONE; TISSUE SCAFFOLD;

ARTICLE; CELL PROLIFERATION; CELL STRUCTURE; CELL VIABILITY; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; ELECTROSPINNING; EMBRYO; HUMAN; HUMAN CELL; IN VITRO STUDY; MOUSE; NONHUMAN; POLYMERIZATION; SCANNING ELECTRON MICROSCOPY; TISSUE REGENERATION; WOUND HEALING;

EID: 84884185633     PISSN: 08839115     EISSN: 15308030     Source Type: Journal    
DOI: 10.1177/0883911513494625     Document Type: Article

References (56)

1. Zhong SP, Zhang YZ, Lim CT. Tissue scaffolds for skin wound healing and dermal reconstruction. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010 ; 2: 510-525
2. Chong EJ, Phan TT, Lim IJ, et al. Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution. Acta Biomater. 2007 ; 3: 321-330
3. Clark RA, Ghosh K, Tonnesen MG. Tissue engineering for cutaneous wounds. J Invest Dermatol. 2007 ; 127: 1018-1029
4. Sundaramurthi D, Vasanthan KS, Kuppan P, et al. Electrospun nanostructured chitosan-poly(vinyl alcohol) scaffolds: a biomimetic extracellular matrix as dermal substitute. Biomed Mater. 2012 ; 7: 045005
5. Falanga V, Faria K, Langer R, et al Bioengineered skin constructs: principles of tissue engineering. 3 rd ed.Burlington, MA: Academic Press, 2007. Burlington, MA: Academic Press ; 2007 :
6. Detta N, Errico C, Dinucci D, et al. Novel electrospun polyurethane/gelatin composite meshes for vascular grafts. J Mater Sci: Mater Med. 2010 ; 21: 1761-1769
7. Volpato F, Ramos SL, Motta A, et al. Physical and in vitro biological evaluation of a PA 6/MWCNT electrospun composite for biomedical applications. J Bioact Compat Polym. 2011 ; 26: 35-47
8. Guarino V, Alvarez-Perez M, Cirillo V, et al. hMSC interaction with PCL and PCL/gelatin platforms: a comparative study on films and electrospun membranes. J Bioact Compat Polym. 2011 ; 26: 144-160
9. Toncheva A, Spasova M, Paneva D, et al. Drug-loaded electrospun polylactide bundles. J Bioact Compat Polym. 2011 ; 26: 161-172
10. Bianco A, Bozzo BM, Del Gaudio C, et al. Poly(l-lactic acid)/calcium-deficient nanohydroxyapatite electrospun mats for bone marrow stem cell cultures. J Bioact Compat Polym. 2011 ; 26: 225-241
11. Hualin Z. Electrospun poly(lactic-co-glycolic acid)/multiwalled carbon nanotubes composite scaffolds for guided bone tissue regeneration. J Bioact Compat Polym. 2011 ; 26: 347-362
12. Kim SE, Heo D, Lee JB, et al. Electrospun gelatin/polyurethane blended nanofibers for wound healing. Biomed Mater. 2009 ; 4: 044106
13. Grafahrend D, Heffels K-H, Möller M, et al. Electrospun biofunctionalized fibers as tailored in vitro substrates for keratinocyte cell culture. Macromol Biosci. 2010 ; 10: 1022-1027
14. Bhardwaj N, Kundu SC. Electrospinning: a fascinating fiber fabrication technique. Biotechnol Adv. 2010 ; 28: 325-347
15. Dalton PD, Calvet J, Mourran A, et al. Melt electrospinning of poly-(ethylene glycol-block-ε-caprolactone). Biotechnol J. 2006 ; 1: 998-1006
16. Hutmacher DW, Dalton PD. Melt electrospinning. Chem: Asian J. 2011 ; 6: 44-56
17. Pham QP, Sharma U, Mikos AG. Electrospun poly(ε-caprolactone) microfiber and multilayer nanofiber/microfiber scaffolds: characterization of the scaffolds and measurement of cellular infiltration. Biomacromolecules. 2006 ; 7: 2796-2805
18. McKee MG, Unal S, Wilkes GL, et al. Branched polyesters: recent advances in synthesis and performance. Prog Polym Sci. 2005 ; 30: 507-539
19. McKee M, Park T, Unal S, et al. Electrospinning of linear and highly branched segmented poly(urethane urea)s. Polymer. 2005 ; 46: 2011-2015
20. Xie W, Jiang N, Gan Z. Effects of multi-arm structure on crystallization and biodegradation of star-shaped poly(ε-caprolactone). Macromol Biosci. 2008 ; 8: 775-784
21. Hao Q, Li F, Li Q, et al. Preparation and crystallization kinetics of new structurally well-defined star-shaped biodegradable poly(l-lactide)s initiated with diverse natural sugar alcohols. Biomacromolecules. 2005 ; 6: 2236-2247
22. Balakrishnan S, Krishnan M, Dubois P, et al. Kinetic and thermodynamic considerations in the synthesis of a new three-arm poly(ε-caprolactone). Polym Eng Sci. 2004 ; 44: 1491-1497
23. Balakrishnan S, Krishnan M, Narayan R, et al. Three-arm poly(ε-caprolactone) by extrusion polymerization. Polym Eng Sci. 2006 ; 46: 235-240
24. Puppi D, Detta N, Piras AM, et al. Development of electrospun three-arm star poly(ε-caprolactone) meshes for tissue engineering applications. Macromol Biosci. 2010 ; 10: 887-897
25. Puppi D, Piras AM, Chiellini F, et al. Optimized electro- and wet-spinning techniques for the production of polymeric fibrous scaffolds loaded with bisphosphonate and hydroxyapatite. J Tissue Eng Regen Med. 2011 ; 5: 253-263
26. Puppi D, Dinucci D, Bartoli C, et al. Development of 3D wet-spun polymeric scaffolds loaded with antimicrobial agents for bone engineering. J Bioact Compat Polym. 2011 ; 26: 478-492
27. Mota C, Puppi D, Dinucci D, et al. Additive manufacturing of star poly(ε-caprolactone) wet-spun scaffolds for bone tissue engineering applications. J Bioact Compat Polym. 2013 ; 28: 320-340
28. Mota C, Puppi D, Gazzarri M, et al. Melt-electrospinning writing of 3D star poly(ε-caprolactone) scaffolds. Polym Int. 2013 ; 62: 893-900
29. Kirkpatrick CJ, Fuschs S, Unger RE. Coculture systems for vascularization: learning from nature. Adv Drug Deliver Rev. 2011 ; 63: 291-299
30. Karchin A, Simonovsky FI, Ratner BD, et al. Melt electrospinning of biodegradable polyurethane scaffolds. Acta Biomater. 2011 ; 7: 3277-3284
31. Park S, Lee S, Kim WD. Fabrication of porous polycaprolactone/ hydroxyapatite (PCL/HA) blend scaffolds using a 3D plotting system for bone tissue engineering. Bioprocess Biosyst Eng. 34: 505-513
32. Stark HJ, Szabowski A, Fusenig NE, et al. Organotypic cocultures as skin equivalents: a complex and sophisticated in vitro system. Biol Proced Online. 2004 ; 6: 55-60
33. Puppi D, Mota C, Gazzarri M, et al. Additive manufacturing of wet-spun polymeric scaffolds for bone tissue engineering. Biomed Microdevices. 2012 ; 14: 1115-1127
34. Kudelska-Mazur D, Malgorzata L-S, Mazur M, et al. Osteogenic cell contact with biomaterials influences phenotype expression. Cell Tissue Bank. 2005 ; 6: 55-64
35. Junqueira LCU, Bignolas G, Brentani RR. A simple and sensitive method for the quantitative estimation of collagen. Anal Biochem. 1979 ; 94: 96-99
36. SoperDS. Analysis of variance (ANOVA) calculator - one-way ANOVA from summary data, http://www.danielsoper.com/statcalc3 (2012).
37. Schoop VM, Mirancea N, Fusenig NE. Epidermal organization and differentiation of HaCaT keratinocytes in organotypic coculture with human dermal fibroblasts. J Invest Dermatol. 1999 ; 112: 343-353
38. Peschel G, Dahse HM, Konrad A, et al. Growth of keratinocytes on porous films of poly(3-hydroxybutyrate) and poly(4-hydroxybutyrate) blended with hyaluronic acid and chitosan. J Biomed Mater Res A. 2008 ; 85: 1072-1081
39. Maas-Szabowski N, Szabowski A, Stark HJ, et al. Organotypic cocultures with genetically modified mouse fibroblasts as a tool to dissect molecular mechanisms regulating keratinocyte growth and differentiation. J Invest Dermatol. 2001 ; 116: 816-820
40. Walter MN, Wright KT, Fuller HR, et al. Mesenchymal stem cell-conditioned medium accelerates skin wound healing: an in vitro study of fibroblast and keratinocyte scratch assays. Exp Cell Res. 2010 ; 316: 1271-1281
41. Rheinwald JG, Green H. Seria cultivation of strains of human epidermal keratinocytes: the formation keratinizin colonies from single cells. Cell. 1975 ; 6: 331-343
42. Owen SC, Shoichet MS. Design of three-dimensional biomimetic scaffolds. J Biomed Mater Res A. 2010 ; 94: 1321-1331
43. Kempf M, Miyamura Y, Liu PY, et al. A denatured collagen microfiber scaffold seeded with human fibroblasts and keratinocytes for skin grafting. Biomaterials. 2011 ; 32: 4782-4792
44. Auxefans C, Fradette J, Lequeux C, et al. Evolution of three dimensional skin equivalent models reconstructed in vitro by tissue engineering. Eur J Dermatol. 2009 ; 19: 107-113
45. Huang WY, Yeh CL, Lin JH, et al. Development of fibroblast culture in three-dimensional activated carbon fiber-based scaffold for wound healing. J Mater Sci Mater Med. 2012 ; 23: 1465-1478
46. Chandrasekaran AR, Venugopal J, Sundarrajan S, et al. Fabrication of a nanofibrous scaffold with improved bioactivity for culture of human dermal fibroblasts for skin regeneration. Biomed Mater. 2011 ; 6: 015001
47. Lee DA, Assoku E, Doyle V. A specific quantitative assay for collagen synthesis by cells seeded in collagen-based biomaterials using sirius red F3B precipitation. J Mater Sci Mater Med. 1998 ; 9: 47-51
48. Shariati SRP, Shokrgozar MA, Vossoughi M, et al. In vitro co-culture of human skin keratinocytes and fibroblasts on biocompatible and biodegradable scaffold. Iran Biomed J. 2009 ; 13: 169-177
49. Zhang B, Lalani R, Cheng F, et al. Dual-functional electrospun poly(2-hydroxyethyl methacrylate). J Biomed Mater Res A. 2011 ; 99: 455-466
50. Maas-Szabowski N, Stark HJ, Fusenig NE. Keratinocyte growth regulation in defined organotypic cultures through IL-1-induced keratinocyte growth factor expression in resting fibroblasts. J Invest Dermatol. 2000 ; 114: 1075-1084
51. Sun T, Mai S, Norton D, et al. Self-organization of skin cells in three-dimensional electrospun polystyrene scaffolds. Tissue Eng. 2005 ; 11: 1023-1032
52. Hutmacher DW, Schantz T, Zein I, et al. Mechanical properties and cell cultural response of polycaprolactone scaffolds designed and fabricated via fused deposition modeling. J Biomed Mater Res. 2001 ; 55: 203-216
53. Kundu B, Kundu SC. Silk sericin/polyacrylamide in situ forming hydrogels for dermal reconstruction. Biomaterials. 2012 ; 33: 7456-7467
54. Moll R, Divo M, Langbein L. The human keratins: biology and pathology. Histochem Cell Biol. 2008 ; 129: 705-733
55. Sun T, Norton D, Ryan A, et al. Investigation of fibroblast and keratinocyte cell-scaffold interactions using a novel 3D cell culture system. J Mater Sci Mater Med. 2007 ; 18: 321-328
56. Dutta RC, Dutta AK. Comprehension of ECM-cell dynamics: a prerequisite for tissue regeneration. Biotechnol Adv. 2010 ; 28: 764-769