Unique contribution of the cell wall-binding endoglucanase G to the cellulolytic complex in Clostridium cellulovorans

Applied and Environmental Microbiology

Volumn 79, Issue 19, 2013, Pages 5942-5948

  Jeon, Sang Duck ^a   Lee, Ji Eun ^a   Kim, Su Jung ^a   Park, Sung Hyun ^a   Choi, Gi Wook ^b   Han, Sung Ok ^a  

^a Korea University   (South Korea)

^b Changhae Ethanol Co Ltd   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYTIC COMPONENTS; CELL SURFACE DISPLAYS; CELLULOSE SUBSTRATES; CLOSTRIDIUM CELLULOVORANS; CRYSTALLINE CELLULOSE; IMMUNOFLUORESCENCE MICROSCOPY; SCAFFOLDING PROTEINS; SPECIFIC INTERACTION;

BINDING ENERGY; CELL MEMBRANES; CELLULOSE; CLOSTRIDIUM; ENZYMES; HYDROLYSIS; PROTEINS;

SCAFFOLDS (BIOLOGY);

BIOMARKER; CELLULOSE; ENZYME ACTIVITY; FLUORESCENCE; HYDROLYSIS; PROTEIN; SUBSTRATE; SURFACE LAYER;

BACTERIAL PROTEIN; CARRIER PROTEIN; CELLULASE; CELLULOSE; CELLULOSE BINDING PROTEIN A, CLOSTRIDIUM CELLULOVORANS;

ARTICLE; CELL WALL; CLOSTRIDIUM CELLULOVORANS; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYMOLOGY; FLUORESCENCE MICROSCOPY; HYDROLYSIS; METABOLISM; PROTEIN BINDING; SURFACE PLASMON RESONANCE;

BACTERIAL PROTEINS; CARRIER PROTEINS; CELL WALL; CELLULASE; CELLULOSE; CLOSTRIDIUM CELLULOVORANS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HYDROLYSIS; MICROSCOPY, FLUORESCENCE; PROTEIN BINDING; SURFACE PLASMON RESONANCE;

EID: 84884181164     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01400-13     Document Type: Article

References (32)

1. Fontes CM, Gilbert HJ. 2010. Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates. Annu. Rev. Biochem. 79:655-681.
2. Demain AL, Newcomb M, Wu JH. 2005. Cellulase, clostridia, and ethanol. Microbiol. Mol. Biol. Rev. 69:124-154.
3. Stahl SW, Nash MA, Fried DB, Slutzki M, Barak Y, Bayer EA, Gaub HE. 2012. Single-molecule dissection of the high-affinity cohesin-dockerin complex. Proc. Natl. Acad. Sci. U. S. A. 109:20431-20436.
4. Hyeon JE, Jeon SD, Han SO. 4 April 2013. Cellulosome-based, Clostridium-derived multi-functional enzyme complexes for advanced biotechnology tool development: advances and applications. Biotechnol. Adv. doi:10.1016/j.biotechadv.2013.03.009.
5. Fendri I, Tardif C, Fierobe HP, Lignon S, Valette O, Pages S, Perret S. 2009. The cellulosomes from Clostridium cellulolyticum: identification of new components and synergies between complexes. FEBS J. 276:3076-3086.
6. Sakka K, Sugihara Y, Jindou S, Sakka M, Inagaki M, Kimura T. 2011. Analysis of cohesin-dockerin interactions using mutant dockerin proteins. FEMS Microbiol. Lett. 314:75-80.
7. Craig SJ, Foong FC, Nordon R. 2006. Engineered proteins containing the cohesin and dockerin domains from Clostridium thermocellum provides a reversible, high affinity interaction for biotechnology applications. J. Biotechnol. 121:165-173.
8. Karpol A, Kantorovich L, Demishtein A, Barak Y, Morag E, Lamed R, Bayer EA. 2009. Engineering a reversible, high-affinity system for efficient protein purification based on the cohesin-dockerin interaction. J. Mol. Recognit. 22:91-98.
9. Koukiekolo R, Cho HY, Kosugi A, Inui M, Yukawa H, Doi RH. 2005. Degradation of corn fiber by Clostridium cellulovorans cellulases and hemicellulases and contribution of scaffolding protein CbpA. Appl. Environ. Microbiol. 71:3504-3511.
10. Murashima K, Kosugi A, Doi RH. 2002. Synergistic effects on crystalline cellulose degradation between cellulosomal cellulases from Clostridium cellulovorans. J. Bacteriol. 184:5088-5095.
11. Cho W, Jeon SD, Shim HJ, Doi RH, Han SO. 2010. Cellulosomic profiling produced by Clostridium cellulovorans during growth on different carbon sources explored by the cohesin marker. J. Biotechnol. 145: 233-239.
12. Jeon SD, Lee JE, Kim SJ, Kim SW, Han SO. 2012. Analysis of selective, high protein-protein binding interaction of cohesin-dockerin complex using biosensing methods. Biosens. Bioelectron. 35:382-389.
13. Doi RH, Park JS, Liu CC, Malburg LM, Tamaru Y, Ichiishi A, Ibrahim A. 1998. Cellulosome and noncellulosomal cellulases of Clostridium cellulovorans. Extremophiles 2:53-60.
14. Jeon SD, Yu KO, Kim SW, Han SO. 2012. The processive endoglucanase EngZ is active in crystalline cellulose degradation as a cellulosomal subunit of Clostridium cellulovorans. N. Biotechnol. 29:365-371.
15. Hazlewood GP, Davidson K, Laurie JI, Huskisson NS, Gilbert HJ. 1993. Gene sequence and properties of CelI, a family E endoglucanase from Clostridium thermocellum. J. Gen. Microbiol. 139:307-316.
16. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
17. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
18. Jeon E, Hyeon JE, Suh DJ, Suh YW, Kim SW, Song KH, Han SO. 2009. Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera beta-glucosidase genes. Mol. Cells 28:369-373.
19. Miller GL, BR, Glennon WE, Burton AL. 1960. Measurement of carboxymethyl cellulase activity. Anal. Biochem. 1:127-132.
20. Murashima K, Kosugi A, Doi RH. 2003. Synergistic effects of cellulosomal xylanase and cellulases from Clostridium cellulovorans on plant cell wall degradation. J. Bacteriol. 185:1518-1524.
21. Jeon SD, Yu KO, Kim SW, Han SO. 2011. A celluloytic complex from Clostridium cellulovorans consisting of mannanase B and endoglucanase E has synergistic effects on galactomannan degradation. Appl. Microbiol. Biotechnol. 90:565-572.
22. Kim MJ, Park HS, Seo KH, Yang HJ, Kim SK, Choi JH. 2013. Complete solubilization and purification of recombinant human growth hormone produced in Escherichia coli. PLoS One 8:e56168. doi:10.1371/journal.pone.0056168.
23. Handelsman T, Barak Y, Nakar D, Mechaly A, Lamed R, Shoham Y, Bayer EA. 2004. Cohesin-dockerin interaction in cellulosome assembly: a single Asp-to-Asn mutation disrupts high-affinity cohesin-dockerin binding. FEBS Lett. 572:195-200.
24. Zhao G, Ali E, Sakka M, Kimura T, Sakka K. 2006. Binding of S-layer homology modules from Clostridium thermocellum SdbA to peptidoglycans. Appl. Microbiol. Biotechnol. 70:464-469.
25. Pinheiro BA, Gilbert HJ, Sakka K, Sakka K, Fernandes VO, Prates JAM, Alves VD, Bolam DN, Ferreira LMA, Fontes CMGA. 2009. Functional insights into the role of novel type I cohesin and dockerin domains from Clostridium thermocellum. Biochemical J. 424:375-384.
26. Anderson TD, Robson SA, Jiang XW, Malmirchegini GR, Fierobe HP, Lazazzera BA, Clubb RT. 2011. Assembly of minicellulosomes on the surface of Bacillus subtilis. Appl. Environ. Microbiol. 77:4849-4858.
27. Jindou S, Kajino T, Inagaki M, Karita S, Beguin P, Kimura T, Sakka K, Ohmiya K. 2004. Interaction between a type-II dockerin domain and a type-II cohesin domain from Clostridium thermocellum cellulosome. Biosci. Biotechnol. Biochem. 68:924-926.
28. Doi RH, Tamaru Y. 2001. The Clostridium cellulovorans cellulosome: an enzyme complex with plant cell wall degrading activity. Chem. Rec. 1:24-32.
29. Demishtein A, Karpol A, Barak Y, Lamed R, Bayer EA. 2010. Characterization of a dockerin-based affinity tag: application for purification of a broad variety of target proteins. J. Mol. Recognit. 23:525-535.
30. Kosugi A, Amano Y, Murashima K, Doi RH. 2004. Hydrophilic domains of scaffolding protein CbpA promote glycosyl hydrolase activity and localization of cellulosomes to the cell surface of Clostridium cellulovorans. J. Bacteriol. 186:6351-6359.
31. Kosugi A, Murashima K, Tamaru Y, Doi RH. 2002. Cell-surfaceanchoring role of N-terminal surface layer homology domains of Clostridium cellulovorans EngE. J. Bacteriol. 184:884-888.
32. Karpol A, Barak Y, Lamed R, Shoham Y, Bayer EA. 2008. Functional asymmetry in cohesin binding belies inherent symmetry of the dockerin module: insight into cellulosome assembly revealed by systematic mutagenesis. Biochem. J. 410:331-338.