Synergistic effects on crystalline cellulose degradation between cellulosomal cellulases from Clostridium cellulovorans

Journal of Bacteriology

Volumn 184, Issue 18, 2002, Pages 5088-5095

  Murashima, Koichiro ^a   Kosugi, Akihiko ^a   Doi, Roy H ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; CELLULASE; CELLULOSE; CELLULOSOME; ENDOGLUCANASE ENGE; ENDOGLUCANASE ENGH; EXOGLUCANASE EXGS; GLUCAN SYNTHASE; PROTEIN; RECOMBINANT PROTEIN; SCAFFOLDING PROTEIN CBPA; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL REACTION; CLOSTRIDIUM; CLOSTRIDIUM CELLULOVORANS; COMPLEX FORMATION; CONTROLLED STUDY; CRYSTAL; ENZYMATIC DEGRADATION; ENZYME ACTIVITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; SOLUBILITY;

BACTERIAL PROTEINS; CARRIER PROTEINS; CELLULASE; CELLULOSE; CELLULOSE 1,4-BETA-CELLOBIOSIDASE; CLOSTRIDIUM; CRYSTALLIZATION; GLYCOSIDE HYDROLASES; MULTIENZYME COMPLEXES; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM; CLOSTRIDIUM CELLULOVORANS; POSIBACTERIA;

EID: 0036723835     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.184.18.5088-5095.2002     Document Type: Article

References (47)

1. Cellulosome structure and ultrastructure
2. Digestion of crystalline cellulose substrates by the Clostridium thermocellum cellulosome: Structural and morphological aspects
3. Optimized mixtures of recombinant Humicola insolens cellulases for the biodegradation of crystalline cellulose
4. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
5. Cellulose-binding domains promote hydrolysis of different sites on crystalline cellulose
6. Dissociation of the cellulosome of Clostridium thermocellum in the presence of ethylenediaminetetraacetic acid occurs with the formation of truncated polypeptides
7. C1-Cx revisited: Intramolecular synergism in a cellulase
8. Non-hydrolytic disruption of cellulose fibers by the binding domain of a bacterial cellulase
9. Design and production of active cellulosome chimeras. Selective incorporation of dockerin-containing enzymes into defined functional complexes
10. CelG from Clostridium cellulolyticum: A multidomain endoglucanase acting efficiently on crystalline cellulose
11. Synergism between the cellulosome-integrating protein CipA and endoglucanase CelD of Clostridium thermocellum
12. New families in the classification of glycosyl hydrolases based on amino acid sequence similarities
13. Updating the sequence-based classification of glycosyl hydrolases
14. Cellulase families revealed by hydrophobic cluster analysis
15. Synergism of cellulases from Trichoderma reesei in the degradation of cellulose
16. Characterization of EngF from Clostridium cellulovorans and identification of a novel cellulose binding domain
17. Saccharification of complex cellulosic substrates by the cellulase system from Clostridium thermocellum
18. Interaction between Clostridium thermocellum endoglucanase CelD and polypeptides derived from the cellulosome-integrating protein CipA: Stoichiometry and cellulolytic activity of the complexes
19. Cell surface anchoring role of N-terminal surface layer homology domains of Clostridium cellulovorans EngE
20. The anchorage function of CipA (CelL), a scaffolding protein of the Clostridium thermocellum cellulosome
21. Exoglucanase activities of the recombinant Clostridium thermocellum CelS, a major cellulosome component
22. The cellulosome of Clostridium thermocellum
23. Thioredoxin as a fusion partner for production of soluble recombinant proteins in Escherichia coli
24. Properties of eXgs, a gene for a major subunit of the Clostridium cellulovorans cellulosome
25. Cellulose promotes extracellular assembly of Clostridium cellulovorans cellulosomes
26. Heterologous production of Clostridium cellulovorans engB, using protease-deficient Bacillus subtilis, and preparation of active recombinant cellulosomes
27. Determination of subunit composition of Clostridium cellulovorans cellulosomes that degrade plant cell walls
28. Species-specificity of the cohesin-dockerin interaction between Clostridium thermocellum and Clostridium cellulolyticum: Prediction of specificity determinants of the dockerin domain
29. Role of scaffolding protein CipC of Clostridium cellulolyticum in cellulose degradation
30. The crystal structure of the processive endocellulase CelF of Clostridium cellulolyticum in complex with a thiooligosaccharide inhibitor at 2.0 A resolution
31. Crystal structures of the cellulase Cel48F in complex with inhibitors and substrates give insights into its processive action
32. The processive endocellulase CelF, a major component of the Clostridium cellulolyticum cellulosome: Purification and characterization of the recombinant form
33. Intramolecular synergism in an engineered exo-endo-1,4-beta-glucanase fusion protein
34. The cellulosome and cellulose degradation by anaerobic bacteria
35. The cellulosome concept as an efficient microbial strategy for the degradation of insoluble polysaccharides
36. Essential 170-kDa subunit for degradation of crystalline cellulose by Clostridium cellulovorans cellulase
37. Primary sequence analysis of Clostridium cellulovorans cellulose binding protein A (CbpA)
38. Isolation and characterization of an anaerobic, cellulolytic bacterium. Clostridium cellulovorans sp. nov
39. Pectate lyase A, an enzymatic subunit of the Clostridium cellulovorans cellulosome
40. Three surface layer homology domains at the N terminus of the Clostridium cellulovorans major cellulosomal subunit EngE
41. A large gene cluster for the Clostridium cellulovorans cellulosome
42. Crystalline cellulose degradation: New insight into the function of cellobiohydrolases
43. Properties conferred on Clostridium thermocellum endoglucanase CelC by grafting the duplicated segment of endoglucanase CelD
44. Cellulose hydrolysis by bacteria and fungi
45. Method for measuring cellulase activity
46. Two components of an extracellular protein aggregate of Clostridium thermocellum together degrade crystalline cellulose
47. Expression, purification and subunit-binding properties of cohesins 2 and 3 of the Clostridium thermocellum cellulosome