Characterisation and identification of enzyme-producing autochthonous bacteria from the gastrointestinal tract of two Indian air-breathing fish

Beneficial Microbes

Volumn 4, Issue 3, 2013, Pages 277-284

  Banerjee, G ^a   Ray, A K ^a   Askarian, F ^b   Ringo E ^b  

^a VISVA BHARATI UNIVERSITY   (India)

^b UNIVERSITY OF TROMSØ   (Norway)

Author keywords

Air breathing fish; Bacteria; Enzyme production; Identification; Intestine

Indexed keywords

AMYLASE; BACTERIAL ENZYME; CELLULASE; RNA 16S;

ARTICLE; AUTOCHTHONOUS BACTERIUM; BACILLUS; BACILLUS LICHENIFORMIS; BACILLUS SUBTILIS; BACTERIAL COUNT; BACTERIAL ENDOSPORE; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; CATFISH; CHANNA PUNCTATUS; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME SYNTHESIS; FISH; GENE SEQUENCE; GRAM POSITIVE BACTERIUM; HETEROPNEUSTES FOSSILIS; INDIA; INTESTINE; INTESTINE FLORA; MURREL; NONHUMAN; PH; SEQUENCE ANALYSIS; TEMPERATURE;

EID: 84884177547     PISSN: 18762883     EISSN: 18762891     Source Type: Journal    
DOI: 10.3920/BM2012.0051     Document Type: Article

References (39)

1. Askarian, F., Zhou, Z., Olsen, R.E., Sperstad, S. and Ringø, E. 2012. Culturable autochthonous gut bacteria in Atlantic salmon (Salmo salar L.) fed diets with or without chitin. Characterization by 16S rRNA gene sequencing, ability to produce enzymes and in vitro growth inhibition of four fish pathogens. Aquaculture 326-329: 1-8.
2. Bairagi, A., Sarkar Ghosh, K., Sen, S.K. and Ray, A.K., 2002. Enzyme producing bacterial flora isolated from fish digestive tracts. Aquaculture International 10: 109-121.
3. Bernfeld, P., 1955. Amylase [alpha] and [beta]. In: Kolowick, S.P. and Kaplan, N.O. (eds.) Methods of enzymology. Academic Press, New York, NY, USA, pp. 149-150.
4. Beveridge, M.C.M., Sikdar, P.K., Frerichs, G.N. and Millar, S., 1991. The ingestion of bacteria in suspension by the common carp Cyprinus carpio L. Journal of Fish Biology 39: 825-831.
5. Das, K.M. and Tripathi, S.D., 1991. Studies on the digestive enzymes of grass carp, Ctenopharyngodon idella (Val.). Aquaculture 92: 21-32.
6. Denison, D.A. and Koehn, R.D., 1977. Cellulase activity of Poronia oedipus. Mycologia 69: 592-601.
7. Ghosh, K., Sen, S.K. and Ray, A.K., 2002. Characterization of bacilli isolated from the gut of rohu, Labeo rohita, fingerlings and its significance in digestion. Journal of Applied Aquaculture 12: 33-42.
8. He, S., Zhou, Z., Liu, Y., Cao, Y., Meng, K., Shi, P., Yao, B. and Ringø, E., 2010. Effects of the antibiotic growth promoters xavomycin and xorfenicol on the autochthonous intestinal microbiota of hybrid tilapia (Oreochromis niloticus ?×O. aureus ?). Archives of Microbiology 192: 985-994.
9. Holben, W.E., Williams, P., Saarinen, M., Srkilahti, L.K. and Apajalahti, J.H.A., 2002. Phylogenetic analysis of intestinal microflora indicates a novel Mycoplasma phylotype in farmed and wild salmon. Microbial Ecology 44: 175-185.
10. Hovda, M.B., Lunestad, B.T., Fontanillas, R. and Rosnes, J.T., 2007. Molecular characterization of the intestinal microbiota of farmed Atlantic salmon (Salmo salar L.). Aquaculture 272: 581-588.
11. Huber, I., Spanggaard, B., Appel, K.F., Rossen, L., Nielsen, T. and Gram, L., 2004. Phylogenetic analysis and in situ identification of the intestinal microbial community of rainbow trout (Oncorhynchus mykiss, Walbaum). Journal of Applied Microbiology 96: 117-132.
12. Jacob, M.B. and Gerstein, M.J., 1960. Handbook of microbiology. D. Van Nostrand Co. Inc. Princeton, NJ, USA.
13. Kar, N., Roy, R.N., Sen, S.K. and Ghosh, K., 2008. Isolation and characterization of extracellular enzyme producing bacilli in the digestive tract of rohu, Labeo rohita (Hamilton) and murrel, Channa punctatus (Bloch). Asian Fisheries Science 21: 421-434.
14. Kasana, R.C., Salwan, R., Dhar, H., Dutt, S. and Gulati, A., 2008. A rapid and easy method for the detection of microbial cellulase on agar plates using Gram's iodine. Current Microbiology 57: 503-507.
15. Khan, A. and Ghosh, K., 2012. Characterization and identification of gut associated phytase-producing bacteria in some fresh water fish cultured in pond. Acta Ichthyologica et Piscatoria 42: 37-45.
16. Liu, Y., Zhou, Z., He, S., Yao, B. and Ringø, E., 2011. Microbial diversity in the sediment of a crab pond in Nanjing, China. Aquaculture Research 44: 321-325.
17. Liu, Y., Zhou, Z., Yao, B., Shi, P., He, S., Hølvold, L.B. and Ringø, E., 2008. Effect of intra-peritoneal injection of immune stimulatory substances on allochthonous gut microbiota of Atlantic salmon (Salmo salar L.) determined using denaturing gradient gel electrophoresis. Aquaculture Research 39: 635-646.
18. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J., 1951. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193: 265-275.
19. Merrifield, D.L., Dimitroglou, A., Bradley, G., Baker, R.T.M. and Davies, S.J., 2009. Soybean meal alters autochthonous microbial populations, microvilli morphology and compromises intestinal enterocyte integrity of rainbow trout (Oncorhynchus mykiss Walbaum). Journal of Fish Diseases 32: 755-766.
20. Mondal, S., Roy, T. and Ray, A.K., 2010. Characterization and identification of enzyme-producing bacteria isolated from the digestive tract of bata, Labeo bata. Journal of the World Aquaculture Society 41: 369-377.
21. Mondal, S., Roy, T., Sen, S.K. and Ray, A.K., 2008. Distribution of enzyme-producing bacteria in the digestive tracts of some freshwater fish. Acta Ichthyologica et Piscatoria 38: 1-8.
22. Nayak, S.K., 2010. Role of gastrointestinal microbiota in fish. Aquaculture Research 41: 1553-1573.
23. Patel, J.B., 2001. 16S rRNA gene sequencing for bacterial pathogen identification in the clinical laboratory. Molecular Diagnosis 6: 313-321.
24. Rae, R., Iatsenko, I., Witte, H. and Sommer, R.J., 2010. A subset of naturally isolated Bacillus strains show extreme virulence to the free-living nematodes Caenorhabditis elegans and Pristionchus pacificus. Environmental Microbiology 12: 3007-3021.
25. Ray, A.K., Ghosh, K. and Ringø, E., 2012. Enzyme-producing bacteria isolated from fish gut: a review. Aquaculture Nutrition 18: 465-492.
26. Ray, A.K., Roy, T., Mondal, S. and Ringø, E., 2010. Identification of gut-associated amylase, cellulase and protease-producing bacteria in three species of Indian major carps. Aquaculture Research 41: 1462-1469.
27. Ringø, E., 1993. The effect of chromic oxide (Cr2O3) on anaerobic bacterial populations associated with the epithelial mucosa of Arctic charr (Salvelinus alpinus L.). Canadian Journal of Microbiology 39: 1169-1173.
28. Ringø, E., Sperstad, S., Kraugerud, O.F. and Krogdahl, Å., 2008. Use of 16S rRNA gene sequencing analysis to characterize culturable intestinal bacteria in Atlantic salmon (Salmo salar) fed diets with cellulose or non-starch polysaccharides from soy. Aquaculture Research 39: 1087-1100.
29. Ringø, E., Sperstad, S., Myklebust, R., Mayhew, T.M. and Olsen, R.E., 2006. The effect of dietary inulin on aerobic bacteria associated with hindgut of Arctic charr (Salvelinus alpinus L.). Aquaculture Research 37: 891-897.
30. Ringø, E., Strøm, E. and Tabachek, J.A., 1995. Intestinal micro flora of salmonids: a review. Aquaculture Research 26: 773-789.
31. Ringø, E., Zhou, Z., Olsen, R.E. and Song, S.K., 2012. Use of chitin and krill in aquaculture-the effect on gut microbiota and the immune system: a review. Aquaculture Nutrition 18: 117-131.
32. Roy, T., Mondal, S. and Ray, S.K., 2009. Phytase producing bacteria in the digestive tract of some fresh water fish. Aquaculture Research 40: 344-353.
33. Saha, A.K. and Ray, A.K., 1998. Cellulase activity in rohu fingerlings. Aquaculture International l6: 281-291.
34. Saha, S., Roy, R.N., Sen, S.K. and Ray, A.K., 2006. Characterization of cellulase-producing bacteria from the digestive tract of tilapia, Oreochromis mossambica (Peters) and grass carp, Ctenopharyngodon idella (Valenciennes). Aquaculture Research 37: 380-388.
35. Silvia, G., Estevão, B. and Gabriela, V., 2006. Fish protein hydrolysis by a psychrotrophic marine bacterium isolated from the gut of hake (Merluccius hubbsi). Canadian Journal of Microbiology 52: 1266-1271.
36. Skrodenyte-Arbaciauskiene, V., Sruoga, A. and Butkauskas, D., 2006. Assessment of microbial diversity in the river trout Salmo trutta fario L. intestinal tract identified by partial 16S rRNA gene sequence analysis. Fishery Science 72: 597-602.
37. Sugita, H., Kawasaki, J. and Deguchi, Y., 1997. Production of amylase by the intestinal microflora in cultured fresh water fish. Letters in Microbiology 24: 105-108.
38. Trust, T.J. and Sparrow, R.A.H., 1974. The bacterial flora in the alimentary tract of freshwater salmonid fishes. Canadian Journal of Microbiology 20: 1219-1228.
39. Walter, H.E., 1984. Methods of enzymatic analysis. Verlag Chemie, Weinheim, Germany.