Proteomics of seminal fluid

Proteomics of Human Body Fluids: Principles, Methods, and Applications

Volumn , Issue , 2007, Pages 467-493

  Solomon, Benjamin ^a   Duncan, Mark W ^a  

^a UNIVERSITY OF COLORADO   (United States)

Author keywords

prostasomes; prostate; proteome; proteomics; Seminal fluid; seminal plasma; seminal vesicles; sperm; spermatozoa

Indexed keywords

EID: 84884086136     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/978-1-59745-432-2_22     Document Type: Chapter

References (121)

1. WHO Laboratory Manual for the Examination of Semen and Sperm-Cervical Mucus interactions, 4th ed. Cambridge: Cambridge University Press, 1999.
2. Mann T. The Biochemistry of Semen and of the Male Reproductive Tract. London: Methuen, 1964.
3. Jenkins AD, Turner TT, Howards SS. Physiology of the male reproductive system. Urol Clin North Am 1978;5:437-450.
4. Rui H, Mevag B, Purvis K. Two-dimensional electrophoresis of proteins in various fractions of the human split ejaculate. Int J Androl 1984;7:509-520.
5. Amelar RD, Hotchkiss RS. The split ejaculate: Its use In the management of male infertility. Fertil Steril 1965;16:46-60.
6. Mann T. Biochemistry of semen. In Greep RO, Astwood EB, eds. Handbook of Physiology: Endocrinology. American Physiology Society, Washington, 1975.
7. Arienti G, Saccardi C, Carlini E, Verdacchi R, Palmerini CA. Distribution of lipid and protein in human semen fractions. Clin Chim Acta 1999;289:111-120.
8. Renneberg H, Konrad L, Dammshauser I, Seitz J, Aumuller G. Immunohistochemistry of prostasomes from human semen. Prostate 1997;30:98-106.
9. Lilja H, Oldbring J, Rannevik G, Laurell CB. Seminal vesicle-secreted proteins and their reactions during gelation and liquefaction of human semen. J Clin Invest 1987;80:281-285.
10. Gray S, Huggins C. Electrophoretic analysis of human semen. Proc Soc Exp Biol Med 1942;50:351.
11. Ross V, Moore DH, Miller EG. Proteins of human seminal plasma. J Biol Chem 1942;144:667.
12. Ross V, Miller EG, Moore DH, Sikorski H. Electrophoretic pattern of seminal plasma from some "abnormal" human semens. Proc Soc Exp Biol Med 1943;54:179.
13. Edwards JJ, Tollaksen SL, Anderson NG. Proteins of human semen. I. Twodimensional mapping of human seminal fluid. Clin Chem 1981;27:1335-1340.
14. Ayyagari RR, Fazleabas AT, Dawood MY. Seminal plasma proteins of fertile and infertile men analyzed by two-dimensional electrophoresis. Am J Obstet Gynecol 1987;157:1528-1533.
15. Frenette G, Dube JY, Lazure C, Paradis G, Chretien M, Tremblay RR. The major 40-kDa glycoprotein in human prostatic fluid is identical to Zn-alpha 2-glycoprotein. Prostate 1987;11:257-270.
16. Lee C, Keefer M, Zhao ZW, et al. Demonstration of the role of prostate-specific antigen in semen liquefaction by two-dimensional electrophoresis. J Androl 1989;10:432-438.
17. Naaby-Hansen S, Flickinger CJ, Herr JC. Two-dimensional gel electrophoretic analysis of vectorially labeled surface proteins of human spermatozoa. Biol Reprod 1997;56:771-787.
18. Charrier JP, Tournel C, Michel S, Dalbon P, Jolivet M. Two-dimensional electrophoresis of prostate-specific antigen in sera of men with prostate cancer or benign prostate hyperplasia. Electrophoresis 1999;20:1075-1081.
19. Starita-Geribaldi M, Poggioli S, Zucchini M, et al. Mapping of seminal plasma proteins by two-dimensional gel electrophoresis in men with normal and impaired spermatogenesis. Mol Hum Reprod 2001;7:715-722.
20. Starita-Geribaldi M, Roux F, Garin J, Chevallier D, Fenichel P, Pointis G. Development of narrow immobilized pH gradients covering one pH unit for human seminal plasma proteomic analysis. Proteomics 2003;3:1611-1619.
21. Fung KY, Glode LM, Green S, Duncan MW. A comprehensive characterization of the peptide and protein constituents of human seminal fluid. Prostate 2004;61:171-181.
22. Kausler W, Spiteller G. Analysis of peptides of human seminal plasma by mass spectrometry. Biol Mass Spectrom 1992;21:567-575.
23. Robert M, Gibbs BF, Jacobson E, Gagnon C. Characterization of prostate-specific antigen proteolytic activity on its major physiological substrate, the sperm motility inhibitor precursor/semenogelin I. Biochemistry 1997;36:3811-3819.
24. Li CH, Hammonds RG Jr, Ramasharma K, Chung D. Human seminal alpha inhibins: Isolation, characterization, and structure. Proc Natl Acad Sci U S A 1985; 82:4041-4044.
25. Lilja H, Abrahamsson PA, Lundwall A. Semenogelin, the predominant protein in human semen. Primary structure and identification of closely related proteins in the male accessory sex glands and on the spermatozoa. J Biol Chem 1989;264: 1894-1900.
26. Goverde HJ, Bisseling JG, Wetzels AM, et al. A neuropeptide in human semen: Oxytocin. Arch Androl 1998;41:17-22.
27. O'Mahony OA, Djahanbahkch O, Mahmood T, Puddefoot JR, Vinson GP. Angiotensin II in human seminal fluid. Hum Reprod 2000;15:1345-1349.
28. Fraioli F, Fabbri A, Gnessi L, et al. Beta-endorphin, Met-enkephalin, and calcitonin in human semen: Evidence for a possible role in human sperm motility. Ann NY Acad Sci 1984;438:365-370.
29. Foresta C, Caretto A, Indino M, Betterle C, Scandellari C. Calcitonin in human seminal plasma and its localization on human spermatozoa. Andrologia 1986;18: 470-473.
30. Cockle SM, Aitken A, Beg F, Morrell JM, Smyth DG. The TRH-related peptide pyroglutamyglutamylprolinamide is present in human semen. FEBS Lett 1989; 252:113-117.
31. Cockle SM, Prater GV, Thetford CR, Hamilton C, Malone PR, Mundy AR. Peptides related to thyrotrophin-releasing hormone (TRH) in human prostate and semen. Biochim Biophys Acta 1994;1227:60-66.
32. Khan Z, Aitken A, Garcia JR, Smyth DG. Isolation and identification of two neutral thyrotropin releasing hormone-like peptides, pyroglutamylphenylalanineproline amide and pyroglutamylglutamineproline amide, from human seminal fluid. J Biol Chem 1992;267:7464-7469.
33. Smith ML, Luqman WA. Prolactin in seminal fluid. Arch Androl 1982;9:105-113.
34. Winslow JW, Shih A, Bourell JH, et al. Human seminal relaxin is a product of the same gene as human luteal relaxin. Endocrinology 1992;130:2660-2668.
35. Fraser LR, Osiguwa OO. Human sperm responses to calcitonin, angiotensin II and fertilization-promoting peptide in prepared semen samples from normal donors and infertility patients. Hum Reprod 2004;19:596-606.
36. Unlu M, Morgan ME, Minden JS. Difference gel electrophoresis: A single gel method for detecting changes in protein extracts. Electrophoresis 1997;18: 2071-2077.
37. Aumuller G, Riva A. Morphology and functions of the human seminal vesicle. Andrologia 1992;24:183-196.
38. Gonzales GF. Function of seminal vesicles and their role on male fertility. Asian J Androl 2001;3:251-258.
39. Lilja H, Laurell CB. The predominant protein in human seminal coagulate. Scand J Clin Lab Invest 1985;45:635-641.
40. Robert M, Gagnon C. Semenogelin I: A coagulum forming, multifunctional seminal vesicle protein. Cell Mol Life Sci 1999;55:944-960.
41. Bjartell A, Malm J, Moller C, Gunnarsson M, Lundwell A. Distribution and tissue expression of semenogelin I and II in man as demonstrated by in situ hybridization and immunocytochemistry. J Androl 1996;17:17-26.
42. Hermans JM, Jones R, Stone SR. Rapid inhibition of the sperm protease acrosin by protein C inhibitor. Biochemistry 1994;33:5440-5444.
43. Laurell M, Christensson A, Abrahamsson PA, Stenflo J, Lilja H. Protein C inhibitor in human body fluids. Seminal plasma is rich in inhibitor antigen deriving from cells throughout the male reproductive system. J Clin Invest 1992; 89:1094-1101.
44. Calderon I, Barak M, Abramovici H, et al. The use of a seminal vesicle specific protein (MHS-5 antigen) for diagnosis of agenesis of vas deferens and seminal vesicles in azoospermic men. J Androl 1994;15:603-607.
45. Kise H, Nishioka J, Satoh K, Okuno T, Kawamura J, Suzuki K. Measurement of protein C inhibitor in seminal plasma is useful for detecting agenesis of seminal vesicles or the vas deferens. J Androl 2000;21:207-212.
46. Sato I, Kojima K, Yamasaki T, et al. Rapid detection of semenogelin by one-step immunochromatographic assay for semen identification. J Immunol Methods 2004;287:137-145.
47. Nylander G. The electrophoretic pattern of prostatic proteins in normal and pathologic secretion. Acta Chir Scand 1955;109:473-482.
48. Atanasov NA, Gikov DG. Disc electrophoresis of prostatic fluid. Relative mobility and molecular weight of some enzymes and proteins. Clin Chim Acta 1972;36:213-221.
49. Resnick MI, Stubbs AJ. Age specific electrophoretic patterns of prostatic fluid. J Surg Res 1978;24:415-420.
50. Carter DB, Resnick MI. High resolution analysis of human prostatic fluid by twodimensional electrophoresis. Prostate 1982;3:27-33.
51. Tsai YC, Harrison HH, Lee C, Daufeldt JA, Oliver L, Grayhack JT. Systematic characterization of human prostatic fluid proteins with two-dimensional electrophoresis. Clin Chem 1984;30:2026-2030.
52. Lee C, Tsai Y, Sensibar J, Oliver L, Grayhack JT. Two-dimensional characterization of prostatic acid phosphatase, prostatic specific antigen and prostate binding protein in expressed prostatic fluid. Prostate 1986;9:135-146.
53. Grover PK, Resnick MI. Analysis of prostatic fluid: Evidence for the presence of a prospective marker for prostatic cancer. Prostate 1995;26:12-18.
54. Guevara J Jr, Herbert BH, Lee C. Two-dimensional electrophoretic analysis of human prostatic fluid proteins. Cancer Res 1985;45:1766-1771.
55. Watt KW, Lee PJ, M'Timkulu T, Chan WP, Loor R. Human prostate-specific antigen: Structural and functional similarity with serine proteases. Proc Natl Acad Sci U S A 1986;83:3166-3170.
56. Iwakiri J, Granbois K, Wehner N, Graves HC, Stamey T. An analysis of urinary prostate specific antigen before and after radical prostatectomy: Evidence for secretion of prostate specific antigen by the periurethral glands. J Urol 1993;149: 783-786.
57. Breul J, Pickl U, Schaff J. Extraprostatic production of prostate specific antigen is under hormonal control. J Urol 1997;157:212-213.
58. Christensson A, Lilja H. Complex formation between protein C inhibitor and prostate-specific antigen in vitro and in human semen. Eur J Biochem 1994; 220:45-53.
59. Sensabaugh GF. Isolation and characterization of a semen-specific protein from human seminal plasma: A potential new marker for semen identification. J Forensic Sci 1978;23:106-115.
60. Wang MC, Papsidero LD, Kuriyama M, Valenzuela LA, Murphy GP, Chu TM. Prostate antigen: A new potential marker for prostatic cancer. Prostate 1981;2:89-96.
61. Lovgren J, Valtonen-Andre C, Marsal K, Lilja H, Lundwall A. Measurement of prostate-specific antigen and human glandular kallikrein 2 in different body fluids. J Androl 1999;20:348-355.
62. Catalona WJ, Smith DS, Ratliff TL, et al. Measurement of prostate-specific antigen in serum as a screening test for prostate cancer. N Engl J Med 1991;324:1156-1161.
63. Huber PR, Schnell Y, Hering F, Rutishauser G. Prostate specific antigen. Experimental and clinical observations. Scand J Urol Nephrol Suppl 1987;104: 33-39.
64. Isono T, Tanaka T, Kageyama S, Yoshiki T. Structural diversity of cancer-related and non-cancer-related prostate-specific antigen. Clin Chem 2002;48:2187-2194.
65. Romas NA, Kwan DJ. Prostatic acid phosphatase. Biomolecular features and assays for serum determination. Urol Clin North Am 1993;20:581-588.
66. Ulvsback M, Lindstrom C, Weiber H, Abrahamsson PA, Lilja H, Lundwall A. Molecular cloning of a small prostate protein, known as beta-microsemenoprotein, PSP94 or beta-inhibin, and demonstration of transcripts in non-genital tissues. Biochem Biophys Res Commun 1989;164:1310-1315.
67. Dube JY, Frenette G, Paquin R, et al. Isolation from human seminal plasma of an abundant 16-kDa protein originating from the prostate, its identification with a 94-residue peptide originally described as beta-inhibin. J Androl 1987;8: 182-189.
68. Tremblay RR, Coulombe E, Cloutier S, et al. Assessment of the trypsin-like human prostatic kallikrein, also known as hK2, in the seminal plasma of infertile men: respective contributions of an ELISA procedure and of Western blotting. J Lab Clin Med 1998;131:330-335.
69. Szecsi PB, Halgreen H, Wong RN, Kjaer T, Tang J. Cellular origin, complementary deoxyribonucleic acid and N-terminal amino acid sequences of human seminal progastricsin. Biol Reprod 1995;53:227-233.
70. Mikolajczyk SD, Millar LS, Marker KM, et al. Seminal plasma contains "BPSA, " a molecular form of prostate-specific antigen that is associated with benign prostatic hyperplasia. Prostate 2000;45:271-276.
71. Ronquist G, Brody I, Gottfries A, Stegmayr B. An Mg2and Ca2?-stimulated adenosine triphosphatase in human prostatic fluid-part II. Andrologia 1978;10:427-433.
72. Arvidson G, Ronquist G, Wikander G, Ojteg AC. Human prostasome membranes exhibit very high cholesterol/phospholipid ratios yielding high molecular ordering. Biochim Biophys Acta 1989;984:167-173.
73. Arienti G, Carlini E, Polci A, Cosmi EV, Palmerini CA. Fatty acid pattern of human prostasome lipid. Arch Biochem Biophys 1998;358:391-395.
74. Ronquist G, Brody I. The prostasome: Its secretion and function in man. Biochim Biophys Acta 1985;822:203-218.
75. Kravets FG, Lee J, Singh B, Trocchia A, Pentyala SN, Khan SA. Prostasomes: current concepts. Prostate 2000;43:169-174.
76. Saez F, Frenette G, Sullivan R. Epididymosomes and prostasomes: Their roles in posttesticular maturation of the sperm cells. J Androl 2003;24:149-154.
77. Arienti G, Carlini E, Saccardi C, Palmerini CA. Role of human prostasomes in the activation of spermatozoa. J Cell Mol Med 2004;8:77-84.
78. Ronquist G, Nilsson BO. The Janus-faced nature of prostasomes: Their pluripotency favours the normal reproductive process and malignant prostate growth. Prostate Cancer Prostatic Dis 2004;71:21-31.
79. Stewart AB, Anderson W, Delves G, Lwaleed BA, Birch B, Cooper A. Prostasomes: A role in prostatic disease? BJU Int 2004;94:985-989.
80. Ronquist G. Nilsson B.O.Hjerten S. Interaction between prostasomes and spermatozoa from human semen, Arch Androl 1990, 24 147-157.
81. Carlini E, Palmerini CA, Cosmi EV, Arienti G. Fusion of sperm with prostasomes: Effects on membrane fluidity. Arch Biochem Biophys 1997;343:6-12.
82. Arienti G, Carlini E, Palmerini CA. Fusion of human sperm to prostasomes at acidic pH. J Membr Biol 1997;155:89-94.
83. Arienti G, Carlini E, Verdacchi R, Cosmi EV, Palmerini CA. Prostasome to sperm transfer of CD13/aminopeptidase N (EC 3.4.11.2). Biochim Biophys Acta 1997; 1336:533-538.
84. Arienti G, Carlini E, Verdacchi R, Palmerini CA. Transfer of aminopeptidase activity from prostasomes to sperm. Biochim Biophys Acta 1997;1336: 269-274.
85. Lindahl M, Tagesson C, Ronquist G. Phospholipase A2 activity in prostasomes from human seminal plasma. Urol Int 1987;42:385-389.
86. Carlsson L, Ronquist G, Nilsson BO, Larsson A. Dominant prostasome immunogens for sperm-agglutinating autoantibodies of infertile men. J Androl 2004;25: 699-705.
87. Carlsson L, Nilsson BO, Ronquist G, Lundquist M, Larsson A. A new test for immunological infertility: An ELISA based on prostasomes. Int J Androl 2004; 27:130-133.
88. Utleg AG, Yi EC, Xie T, et al. Proteomic analysis of human prostasomes. Prostate 2003;56:150-161.
89. Schrimpf SP, Hellman U, Carlsson L, Larsson A, Ronquist G, Nilsson BO. Identification of dipeptidyl peptidase IV as the antigen of a monoclonal antiprostasome antibody. Prostate 1999;38:35-39.
90. Fernandez D, Valdivia A, Irazusta J, Ochoa C, Casis L. Peptidase activities in human semen. Peptides 2002;23:461-468.
91. Lilja H, Weiber H. gamma-Glutamyltransferase bound to prostatic subcellular organelles and in free form in human seminal plasma. Scand J Clin Lab Invest 1983;43:307-312.
92. Mann T, Lutwak-Mann C. Male Reproductive Function and Semen. Berlin: Springer Verlag, 1981.
93. Millette CF. Spermatozoa. In: Knobil E, Neill JD, eds. Encyclopedia of Reproduction. Academic Press, San Diego, CA, 1999:586-596.
94. Mack SR, Zaneveld LJ, Peterson RN, Hunt W, Russell LD. Characterization of human sperm plasma membrane: glycolipids and polypeptides. J Exp Zool 1987; 243:339-346.
95. Morgentaler A, Schopperle WM, Crocker RH, DeWolf WC. Protein differences between normal and oligospermic human sperm demonstrated by two-dimensional gel electrophoresis. Fertil Steril 1990;54:902-905.
96. Naaby-Hansen S. Electrophoretic map of acidic and neutral human spermatozoal proteins. J Reprod Immunol 1990;17:167-185.
97. Primakoff P, Lathrop W, Bronson R. Identification of human sperm surface glycoproteins recognized by autoantisera from immune infertile men, women, and vasectomized men. Biol Reprod 1990;42:929-942.
98. Kritsas JJ, Schopperle WM, DeWolf WC, Morgentaler A. Rapid high resolution two-dimensional electrophoresis of human sperm proteins. Electrophoresis 1992;13:445-449.
99. Xu C, Rigney DR, Anderson DJ. Two-dimensional electrophoretic profile of human sperm membrane proteins. J Androl 1994;15:595-602.
100. Shetty J, Naaby-Hansen S, Shibahara H, Bronson R, Flickinger CJ, Herr JC. Human sperm proteome: Immunodominant sperm surface antigens identified with sera from infertile men and women. Biol Reprod 1999;61:61-69.
101. Bohring C, Krause W. The characterization of human spermatozoa membrane proteins-surface antigens and immunological infertility. Electrophoresis 1999; 20:971-976.
102. Shetty J, Diekman AB, Jayes FC, et al. Differential extraction and enrichment of human sperm surface proteins in a proteome: Identification of immunocontraceptive candidates. Electrophoresis 2001;22:3053-3066.
103. Bohring C, Krause E, Habermann B, Krause W. Isolation and identification of sperm membrane antigens recognized by antisperm antibodies, and their possible role in immunological infertility disease. Mol Hum Reprod 2001;7:113-118.
104. Shibahara H, Sato I, Shetty J, et al. Two-dimensional electrophoretic analysis of sperm antigens recognized by sperm immobilizing antibodies detected in infertile women. J Reprod Immunol 2002;53:1-12.
105. Hao Z, Wolkowicz MJ, Shetty J, et al. SAMP32, a testis-specific, isoantigenic sperm acrosomal membrane-associated protein. Biol Reprod 2002;66:735-744.
106. Lefievre L, Barratt CL, Harper CV, et al. Physiological and proteomic approaches to studying prefertilization events in the human. Reprod Biomed Online 2003;7:419-427.
107. Bohring C, Krause W. Characterization of spermatozoa surface antigens by antisperm antibodies and its influence on acrosomal exocytosis. Am J Reprod Immunol 2003;50:411-419.
108. Ficarro S, Chertihin O, Westbrook VA, et al. Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation. J Biol Chem 2003;278:11, 579-11, 589.
109. Pixton KL, Deeks ED, Flesch FM, et al. Sperm proteome mapping of a patient who experienced failed fertilization at IVF reveals altered expression of at least 20 proteins compared with fertile donors: case report. Hum Reprod 2004;19:1438-447.
110. Primakoff P, Myles DG. Penetration, adhesion, and fusion in mammalian spermegg interaction. Science 2002;296:2183-2185.
111. Bohring C, Krause W. Immune infertility: Towards a better understanding of sperm (auto)-immunity. The value of proteomic analysis. Hum Reprod 2003;18:915-924.
112. Herr JC. Update on the Center for Recombinant Gamete Contraceptive Vaccinogens. Am J Reprod Immunol 1996;35:184-189.
113. Naz RK, Leslie MH. Sperm surface protein profiles of fertile and infertile men: Search for a diagnostic molecular marker. Arch Androl 1999;43:173-181.
114. Rajeev SK, Reddy KVR. Sperm membrane protein profiles of fertile and infertile men: Identification and characterization of fertility-associated sperm antigen. Hum Reprod 2004;19:234-242.
115. Pena P, Risopatron J, Villegas J, Miska W, Schill WB, Sanchez R. Alpha-glucosidase in the human epididymis: Topographic distribution and clinical application. Andrologia 2004;36:315-320.
116. Graves HC, Sensabaugh GF, Blake ET. Postcoital detection of a male-specific semen protein. Application to the investigation of rape. N Engl J Med 1985;312: 338-343.
117. Hochmeister MN, Budowle B, Rudin O, et al. Evaluation of prostate-specific antigen (PSA) membrane test assays for the forensic identification of seminal fluid. J Forensic Sci 1999;44:1057-1060.
118. Simich JP, Morris SL, Klick RL, Rittenhouse-Diakun K. Validation of the use of a commercially available kit for the identification of prostate specific antigen (PSA) in semen stains. J Forensic Sci 1999;44:1229-1231.
119. Ablin RJ, Soanes WA, Bronson P, Witebsky E. Precipitating antigens of the normal human prostate. J Reprod Fertil 1970;22:573-574.
120. Wang MC, Valenzuela LA, Murphy GP, Chu TM. Purification of a human prostate specific antigen. Invest Urol 1979;17:159-163.
121. Papsidero LD, Wang MC, Valenzuela LA, Murphy GP, Chu TM. A prostate antigen in sera of prostatic cancer patients. Cancer Res 1980;40:2428-2432.