Assessment of the trypsin-like human prostatic kallikrein, also known as hK2, in the seminal plasma of infertile men: Respective contributions of an ELISA procedure and of western blotting

Journal of Laboratory and Clinical Medicine

Volumn 131, Issue 4, 1998, Pages 330-335

  Tremblay, R R ^a,b   Coulombe, E ^a   Cloutier, S ^a   Brunet, C ^a   Deperthes, D ^a   Frenette, G ^a   Dube J Y ^a  

^a Research Center   (Canada)

^b UNIVERSITÉ LAVAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME LINKED IMMUNOSORBENT ASSAY; INFERTILITY; KALLIKREIN; MONOCLONAL ANTIBODY; OBSTRUCTIVE AZOOSPERMIA; OLIGOASTHENOTERATOZOOSPERMIA; PROSTATE; PROSTATE FUNCTION; PROSTATE SPECIFIC ANTIGEN; SEMINAL PLASMA; SPERMATOZOON; WESTERN BLOTTING;

EID: 0032055571     PISSN: 00222143     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2143(98)90183-4     Document Type: Article

References (21)

1. Schedlich LJ, Bennetts BH, Morris BJ. Primary structure of human glandular kallikrein gene. DNA 1987;6:429-37.
2. Young CYF, Andrews PE, Montgomery BT, Tindall DJ. Tissue-specific and hormonal regulation of human prostate-specific glandular kallikrein. Biochemistry 1992;31:818-24.
3. Hara M, Koyanagi Y, Inoue T, Fukuyama T. Some physico- chemical characteristics of γ-seminoprotein, an antigenic component specific for human seminal plasma. Jpn J Legal Med 1971;25:322-4.
4. Sensabaugh GF, Crim D. Isolation and characterization of semen-specific protein from human seminal plasma: a potent new marker for semen identification. J Forensic Sci 1978;23:108-15.
5. Deperthes D, Chapdelaine P, Tremblay RR, Brunet C, Berton J, Hébert J, et al. Isolation of prostatic kallikrein hK2, also known as hK1, in human seminal plasma. Biochim Biophys Acta 1995;1245:311-6.
6. Frenette G, Deperthes D, Tremblay RR, Lazure C, Dubé JY. Purification of enzymatically active kallikrein hK2 from human seminal plasma. Biochim Biophys Acta 1997;1334:109-15.
7. Deperthes D, Frenette G, Brillard-Bourdet M, Bourgeois L, Gauthier F, Tremblay RR, et al. Potential involvement of kallikrein hK2 in the hydrolysis of the human seminal vesicle protein after ejaculation. J Androl 1996;17:659-65.
8. Zarghami N, Diamandis E. Detection of prostate-specific antigen mRNA and protein in breast tumors. Clin Chem 1996;42:361-6.
9. Filela X, Molina J, Alcover J, Menenez V, Gimenez N, Carretero P, et al. Prostate-specific antigen detection by ultrasensitive assay in samples from women. Prostate 1996;29:311-6.
10. Oesterling JE. Prostate specific antigen: a critical assessment of the most useful tumor marker for adenocarcinoma of the prostate. J Urol 1991;145:907-23.
11. Gibson TLB, Tremblay RR, Dubé JY, Cohen P. Human kallikrein 2 is a potent insulin-like growth factor binding protein protease. Proceedings of The Endocrine Society, June 1997.
12. Richardson S, Frenette G, Lazure C, Mailloux J, Dubé JY, Tremblay RR. Effect of prostatic kallikreins on the antiprotease system of the female cervix. Proc Can Fed Biol Soc, June 1997.
13. Davidson I, Henry JB. Clinical diagnosis by laboratory methods. Philadelphia: WB Saunders, 1974.
14. Mann T. The biochemistry of semen and of the male reproductive tract. London: Methuen & Co, 1964:193.
15. Ahlgren G, Rannevik G, Lilja H. Impaired secretory function of the prostate in men with oligoasthenozoospermia. J Androl 1995;16:491-8.
16. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T-4. Nature 1970;227:680-8.
17. Henttu P, Lukkarinen O, Vihko P. Expression of the gene coding for human prostate-specific antigen and related hGK-1 in benign and malignant tumors of the human prostate. Int J Cancer 1990;45:654-60.
18. Espana F, Sanchez-Cuenza J, Vera CD, Estelles A, Gilabert J. A quantitative ELISA for the measurement of complexes of prostate-specific antigen with protein C inhibitor when using a purified standard. J Lab Clin Med 1993;122:711-9.
19. Zhang WM, Leinomen J, Kalkkinen N, Dowell B, Stenman UH. Purification and characterization of different molecular forms of prostate-specific antigen in human seminal fluid. Clin Chem 1995;41:1567-73.
20. Christensson A, Lilja H. Complex formation between PCI inhibitor and prostate-specific antigen in vitro in human semen. Eur J Biochem 1994;220:45-53.
21. Deperthes D, Frenette G, Tremblay RR, Dubé JY. Binding of prostatic kallikrein hK2 to protein C inhibitor in human