High level expression, efficient purification and bioactivity assay of recombinant human platelet-derived growth factor AA dimer (PDGF-AA) from methylotrophic yeast Pichia pastoris

Protein Expression and Purification

Volumn 91, Issue 2, 2013, Pages 221-227

  Li, Hongbo ^a,b   Hui, Xiaoyan ^c   Yang, Song ^a   Hu, Xing ^b   Tang, Xiaofeng ^a   Li, Peng ^a   Li, Shiwu ^d   Yang, Lijun ^d   Jin, Shouguang ^d   Wang, Yu ^a,c   Xu, Aimin ^a,c   Wu, Donghai ^a  

^a GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH   (China)

^b HUAIHUA UNIVERSITY   (China)

^c UNIVERSITY OF HONG KONG   (Hong Kong)

^d UNIVERSITY OF FLORIDA   (United States)

Author keywords

Glycosylation; Human platelet derived growth factor A; Pichia pastoris; Protein purification; Recombinant protein

Indexed keywords

PLATELET DERIVED GROWTH FACTOR; PLATELET DERIVED GROWTH FACTOR A; PLATELET-DERIVED GROWTH FACTOR A; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; CELL PROLIFERATION; CELL STRAIN 3T3; CHEMISTRY; DRUG EFFECT; GLYCOSYLATION; HUMAN; HUMAN PLATELET-DERIVED GROWTH FACTOR A; ISOLATION AND PURIFICATION; METABOLISM; MOUSE; PICHIA; PICHIA PASTORIS; PROTEIN PURIFICATION;

GLYCOSYLATION; HUMAN PLATELET-DERIVED GROWTH FACTOR A; PICHIA PASTORIS; PROTEIN PURIFICATION; RECOMBINANT PROTEIN;

ANIMALS; CELL PROLIFERATION; GLYCOSYLATION; HUMANS; MICE; NIH 3T3 CELLS; PICHIA; PLATELET-DERIVED GROWTH FACTOR; RECOMBINANT PROTEINS;

EID: 84883896774     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.08.008     Document Type: Article

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