Minimization of chloroplast contamination in 16S rRNA gene pyrosequencing of insect herbivore bacterial communities

Journal of Microbiological Methods

Volumn 95, Issue 2, 2013, Pages 149-155

  Hanshew, Alissa S ^a   Mason, Charles J ^a   Raffa, Kenneth F ^a   Currie, Cameron R ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

16S rRNA Gene; 454 Pyrosequencing; Chloroplast; Insect; Symbiosis

Indexed keywords

PHOSPHOROTHIOIC ACID; RNA 16S;

16S RRNA GENE; ARTICLE; AZTECA CONSTRUCTOR; CHLOROPLAST; HERBIVORE; HIGH THROUGHPUT SEQUENCING; INSECT; LYMANTRIA DISPAR; MICROBIAL COMMUNITY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PYROSEQUENCING;

16S RRNA GENE; 454 PYROSEQUENCING; CHLOROPLAST; INSECT; SYMBIOSIS;

ANIMALS; BACTERIA; BASE SEQUENCE; DNA PRIMERS; DNA, BACTERIAL; DNA, CHLOROPLAST; HERBIVORY; INSECTS; MOLECULAR SEQUENCE DATA; RNA, RIBOSOMAL, 16S; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

EID: 84883809554     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2013.08.007     Document Type: Article

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