Epitope screening of the PCV2 Cap protein by use of a random peptide-displayed library and polyclonal antibody

Virus Research

Volumn 177, Issue 1, 2013, Pages 103-107

  Ge, Meng ^a   Yan, Ai ^a   Luo, Wei ^a   Hu, Yun Fei ^a   Li, Run Cheng ^a   Jiang, Da Liang ^a   Yu, Xing Long ^a  

^a HUNAN AGRICULTURAL UNIVERSITY   (China)

Author keywords

Capsid protein; Epitope mapping; Phage display; Polyclonal antibodies; Porcine circovirus 2

Indexed keywords

CAPSID PROTEIN; POLYCLONAL ANTIBODY;

AMINO ACID SEQUENCE; ANTIGEN BINDING; ARTICLE; CIRCOVIRUS; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; GENE SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHAGE DISPLAY; PORCINE CIRCOVIRUS 2; PRIORITY JOURNAL;

MIRIDAE; MUS; PORCINE CIRCOVIRUS 2;

CAPSID PROTEIN; EPITOPE MAPPING; PHAGE DISPLAY; POLYCLONAL ANTIBODIES; PORCINE CIRCOVIRUS 2;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, VIRAL; CAPSID PROTEINS; CIRCOVIRIDAE INFECTIONS; CIRCOVIRUS; EPITOPE MAPPING; MICE; MOLECULAR SEQUENCE DATA; PEPTIDE LIBRARY; SWINE; SWINE DISEASES;

EID: 84883782650     PISSN: 01681702     EISSN: 18727492     Source Type: Journal    
DOI: 10.1016/j.virusres.2013.06.018     Document Type: Article

References (27)

1. Blanchard P., Mahé D., Cariolet R., Keranflec'h A., Baudouard M.A., Cordioli P., Albina E., Jestin A. Protection of swine against post-weaning multisystemic wasting syndrome (PMWS) by porcine circovirus type 2 (PCV2) proteins. Vaccine 2003, 21:456-475.
2. Bratkovie T. Progress in phage display: evolution of the technique and its applications. Cellular and Molecular Life Sciences 2010, 67:749-767.
3. Cheung A.K. A stem-loop structure, sequence non-specific, at the origin of DNA replication of porcine circovirus is essential for termination but not for initiation of rolling-circle DNA replication. Virology 2007, 363:229-235.
4. Cheung A.K. Transcriptional analysis of porcine circovirus type 2. Virology 2003, 305:168-180.
5. Ellis J., Hassard L., Clark E., Harding J., Allan G., Willson P., Strokappe J., Martin K., McNeilly F., Meehan B., Todd D., Haines D. Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. Canadian Veterinary Journal 1998, 39:44-51.
6. Fort M., Sibila M., Nofrarías M., Pérez-Martín E., Olvera A., Mateu E., Segalés J. Porcine circovirus type 2 (PCV2) Cap and Rep proteins are involved in the development of cell-mediated immunity upon PCV2 infection. Veterinary Immunology and Immunopathology 2010, 137:226-234.
7. Gamage L.N., Ellis J., Hayes S. Immunogenicity of bacteriophage lambda particles displaying porcine Circovirus 2 (PCV2) capsid protein epitopes. Vaccine 2009, 27:6595-6604.
8. Galfre G., Monaci P., Nicosia A., Luzzago A., Felici F., Cortese R. Immunization with phage-displayed mimotopes. Methods in Enzymology 1996, 267:109-115.
9. Ge M., Luo W., Jiang D., Li R., Zhao W., Chen G., Yang X., Yu X. Development and application of a double-antigen sandwich ELISA for detection of antibodies to porcine circovirus 2. Clinical and Vaccine Immunology 2012, 19:1480-1486.
10. Hayes S., Gamage L.N., Hayes C. Dual expression system for assembling phage lambda display particle (LDP) vaccine to porcine Circovirus 2 (PCV2). Vaccine 2010, 28:6789-6799.
11. Hebert G.A. Ammonium sulfate fractionation of sera: mouse, hamster, guinea pig, monkey, chimpanzee, swine, chicken, and cattle. Applied and Environmental Microbiology 1974, 27:389-393.
12. Jiang L., Zhou J.M., Yin Y., Fang D.Y., Tang Y.X., Jiang L.F. Selection and identification of B-cell epitopes of dengue virus type 2. Virus Research 2010, 150:49-55.
13. Karuppannan A.K., Kwang J. ORF3 of porcine circovirus 2 enhances the in vitro and in vivo spread of the virus. Virology 2011, 410:248-256.
14. Khayat R., Brunn N., Speir J.A., Hardham J.M., Ankenbauer R.G., Schneemann A., Johnson J.E. The 2.3-Angstrom structure of porcine circovirus 2. Journal of Virology 2011, 85:7856-7862.
15. Lefebvre D.J., Costers S., Van Doorsselaere J., Misinzo G., Delputte P.L., Nauwynck H.J. Antigenic differences among porcine circovirus type 2 strains, as demonstrated by the use of monoclonal antibodies. Journal of General Virology 2008, 89:177-187.
16. Lekcharoensuk P., Morozov I., Paul P.S., Thangthumniyom N., Waj-jawalku W., Meng X.J. Epitope mapping of the major capsid protein of type 2 porcine circovirus (PCV2) by using chimeric PCV1 and PCV2. Journal of Virology 2004, 78:8135-8145.
17. Mahé D., Blanchard P., Truong C., Arnauld C., Le Cann P., Cariolet R., Madec F., Albina E., Jestin A. Differential recognition of ORF2 protein from type 1 and type 2 porcine circoviruses and identification of immunorelevant epitopes. Journal of General Virology 2000, 81:1815-1824.
18. Mühle C., Schulz-Drost S., Khrenov A.V., Saenko E.L., Klinge J., Schneider H. Epitope mapping of polyclonal clotting factor VIII-inhibitory antibodies using phage display. Thrombosis and Haemostasis 2004, 91:619-625.
19. Naidu B.R., Ngeow Y.F., Wang L.F., Chan L., Yao Z.J., Pang T. An immunogenic epitope of Chlamydia pneumoniae from a random phage display peptide library is reactive with both monoclonal antibody and patient's sera. Immunology Letters 1998, 62:111-115.
20. Oleksiewicz M.B., Bøtner A., Toft P., Normann P., Storgaard T. Epitope mapping porcine reproductive and respiratory syndrome virus by phage display: the nsp2 fragment of the replicase polyprotein contains a cluster of B-cell epitopes. Journal of Virology 2001, 75:3277-3290.
21. Opriessnig T., Meng X.J., Halbur P.G. Porcine circovirus type 2 associated disease: update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies. Journal of Veterinary Diagnostic Investigation 2007, 19:591-615.
22. Rowley M.J., O'Connor K., Wijeyewickrema L. Phage display for epitope determination: a paradigm for identifying receptor-ligand interactions. Biotechnology Annual Review 2004, 10:151-188.
23. Shang S.B., Jin Y.L., Jiang X.T., Zhou J.Y., Zhang X., Xing G., He J.L., Yan Y. Fine mapping of antigenic epitopes on capsid proteins of porcine circovirus, and antigenic phenotype of porcine circovirus type 2. Molecular Immunology 2009, 46:327-334.
24. Trible B.R., Kerrigan M., Crossland N., Potter M., Faaberg K., Hesse R., Rowland R.R. Antibody recognition of porcine circovirus type 2 capsid protein epitopes after vaccination, infection and disease. Clinical and Vaccine Immunology 2011, 18:749-757.
25. Trible B.R., Rowland R.R. Genetic variation of porcine circovirus type 2 (PCV2) and its relevance to vaccination, pathogenesis and diagnosis. Virus Research 2012, 164(1-2):68-77.
26. Truong C., Mahé D., Blanchard P., Le Dimna M., Madec F., Jestin A., Albina E. Identification of an immunorelevant ORF2 epitope from porcine circovirus type 2 as a serological marker for experimental and natural infection. Archives of Virology 2001, 146:1197-1211.
27. Yang Q.C., Zhang C.Y., Zhao L., Zhou G.H., Wang H.W., Yu L. Identification of a conserved linear epitope on the VP1 protein of serotype O foot-and-mouth disease virus by neutralising monoclonal antibody 8E8. Virus Research 2011, 155:291-299.