Crystal structure of 3-hydroxybenzoate 6-hydroxylase uncovers lipid-assisted flavoprotein strategy for regioselective aromatic hydroxylation

Journal of Biological Chemistry

Volumn 288, Issue 36, 2013, Pages 26235-26245

  Montersino, Stefania ^a   Orru, Roberto ^b,d   Barendregt, Arjan ^c   Westphal, Adrie H ^a   Van Duijn, Esther ^c   Mattevi, Andrea ^b   Van Berkel, Willem J H ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b UNIVERSITY OF PAVIA   (Italy)

^c UTRECHT UNIVERSITY   (Netherlands)

^d EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AROMATIC HYDROXYLATION; BOUND LIPIDS; MONOOXYGENASES; REGIO-SELECTIVE; SOIL MICRO-ORGANISMS; STRUCTURE FEATURES; SUBSTRATE BINDING; SUBSTRATE-BINDING SITES;

AROMATIC COMPOUNDS; PHOSPHOLIPIDS; REGIOSELECTIVITY;

HYDROXYLATION;

3 HYDROXYBENZOATE 6 HYDROXYLASE; 3 HYDROXYBENZOIC ACID; PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLGLYCEROL; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

ARTICLE; CATALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; DIMERIZATION; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; ENZYME SUBUNIT; ESCHERICHIA COLI; HYDROPHILICITY; HYDROXYLATION; MASS SPECTROMETRY; NONHUMAN; OXYGENATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN LIPID INTERACTION; RESIDUE ANALYSIS; STEREOCHEMISTRY; STRUCTURE ANALYSIS;

CRYSTAL STRUCTURE; FLAVOPROTEINS; GENTISATE; HYDROXYLASE; MONOOXYGENASE; OXIDATION-REDUCTION; PHOSPHOLIPID; RHODOCOCCUS;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; FLAVOPROTEINS; GENE EXPRESSION; GENTISATES; HYDROXYLATION; MASS SPECTROMETRY; MIXED FUNCTION OXYGENASES; MUTATION, MISSENSE; NAD; PHOSPHOLIPIDS; RECOMBINANT PROTEINS; RHODOCOCCUS;

EID: 84883693290     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M113.479303     Document Type: Article

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