Detection and differentiation of field and vaccine strains of canine distemper virus using reverse transcription followed by nested real time PCR (RT-nqPCR) and RFLP analysis

Journal of Virological Methods

Volumn 194, Issue 1-2, 2013, Pages 39-45

  Fischer, Cristine Dossin Bastos ^a   Ikuta, Nilo ^a,b   Canal, Cláudio Wageck ^c   Makiejczuk, Aline ^a   Allgayer, Mariangela da Costa ^a   Cardoso, Cristine Hoffmeister ^a   Lehmann, Fernanda Kieling ^a   Fonseca, André Salvador Kazantzi ^b   Lunge, Vagner Ricardo ^a,b  

^a UNIVERSIDADE LUTERANA DO BRASIL   (Brazil)

^b Simbios Biotecnologia   (Brazil)

^c FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

Author keywords

Canine distemper virus; Detection; Diagnosis; Dog; RT PCR

Indexed keywords

DISTEMPER VACCINE; UNCLASSIFIED DRUG; VIRUS NUCLEOPROTEIN; VIRUS VACCINE;

ARTICLE; BLOOD; CANINE DISTEMPER; CANINE DISTEMPER MORBILLIVIRUS; CONJUNCTIVA; CONTROLLED STUDY; DIAGNOSTIC KIT; DIAGNOSTIC TEST ACCURACY STUDY; GENE SEQUENCE; IMMUNOAFFINITY CHROMATOGRAPHY; LABORATORY DEVICE; NC GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RECTUM; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE HOMOLOGY; STRAIN DIFFERENCE; URINE; VALIDATION STUDY; VIRUS DETECTION; VIRUS STRAIN;

CANINE DISTEMPER VIRUS; DETECTION; DIAGNOSIS; DOG; RT-PCR;

ANIMALS; BRAZIL; DISTEMPER; DISTEMPER VIRUS, CANINE; DOG DISEASES; DOGS; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; SENSITIVITY AND SPECIFICITY; VETERINARY MEDICINE; VIRAL VACCINES; VIROLOGY;

EID: 84883440853     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2013.08.002     Document Type: Article

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