Design and utility of temperature-sensitive Gal4 mutants for conditional gene expression in Drosophila

Fly

Volumn 1, Issue 5, 2007, Pages 282-286

  Mondal, Kajari ^a   Vijayraghavan, K ^b   Varadarajan, Raghavan ^a,c  

^a INDIAN INSTITUTE OF SCIENCE   (India)

^b TATA INSTITUTE OF FUNDAMENTAL RESEARCH   (India)

^c JAWAHARLAL NEHRU CENTRE FOR ADVANCED SCIENTIFIC RESEARCH   (India)

Author keywords

Design; Drosophila; Temperature sensitive; Transferability; Yeast

Indexed keywords

DROSOPHILA PROTEIN; ELAV PROTEIN, DROSOPHILA; GAL4 PROTEIN, S CEREVISIAE; HU ANTIGEN; SACCHAROMYCES CEREVISIAE PROTEIN; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ANIMAL; ARTICLE; DROSOPHILA; EYE; FUNGAL GENE; GENE; GENE EXPRESSION; GENETICS; GROWTH, DEVELOPMENT AND AGING; LACTOSE OPERON; MUTATION; PHENOTYPE; REPORTER GENE; TEMPERATURE; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; DROSOPHILA; DROSOPHILA PROTEINS; EYE; GENE EXPRESSION; GENES, FUNGAL; GENES, INSECT; GENES, REPORTER; HU PARANEOPLASTIC ENCEPHALOMYELITIS ANTIGENS; LAC OPERON; MUTATION; PHENOTYPE; SACCHAROMYCES CEREVISIAE PROTEINS; TEMPERATURE; TRANSCRIPTION FACTORS;

EID: 84883390093     PISSN: 19336934     EISSN: 19336942     Source Type: Journal    
DOI: 10.4161/fly.5251     Document Type: Article

References (33)

1. Horowitz NH. Biochemical genetics of Neurospora. Adv Genet 1950; 3:33-71.
2. Nelson HC, Sauer RT. Lambda repressor mutations that increase the affinity and specificity of operator binding. Cell 1985; 42:549-58.
3. Suzuki DT, Grigliatti T, Williamson R. Temperature-sensitive mutations in Drosophila melanogaster. VII. A mutation (para-ts) causing reversible adult paralysis. Proc Natl Acad Sci USA 1971; 68:68-93.
4. Muir RS, Flores H, Zinder ND, Model P, Soberon X, Heitman J. Temperature-sensitive mutants of the EcoRI endonuclease. J Mol Biol 1997; 274:722-37.
5. Patrick M, Duncan R, Coombs KM. Generation and genetic characterization of avian reovirus temperature sensitive mutants. Virology 2001; 284:113-22.
6. Tsai AY, Itoh M, Streuli M, Thai T, Saito H. Isolation and characterization of temperaturesensitive and thermostable mutants of the human receptor-like protein tyrosine phosphatase LAR. J Biol Chem 1991; 266:10534-43.
7. Harris SD, Cheng J, Pugh TA, Pringle JR. Molecular analysis of Saccharomyces cerevisiae chromosome I: On the number of genes and the identification of essential genes using temperature-sensitive-lethal mutations. J Mol Biol 1992; 225:53-65.
8. Dohmen RJ, Wu P, Varshavsky A. Heat-inducible degron: A method for constructing temperature-sensitive mutants. Science 1994; 263:1273-6.
9. Kanemaki M, Sanchez-Diaz A, Gambus A, Labib K. Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo. Nature 2003; 423:720-4.
10. Zeidler MP, Tan C, Bellaiche Y, Cherry S, Hader S, Gayko U, Perrimon N. Temperaturesensitive control of protein activity by conditionally splicing inteins. Nat Biotechnol 2004; 22:871-6.
11. McGuire SE, Le PT, Osborn AJ, Matsumoto K, Davis RL. Spatiotemporal rescue of memory dysfunction in Drosophila. Science 2003; 302:1765-8.
12. Simon MA, Bowtell DD, Dodson GS, Laverty TR, Rubin GM. Ras1 and a putative guanine nucleotide exchange factor perform crucial steps in signaling by the sevenless protein tyrosine kinase. Cell 1991; 67:701-16.
13. Varadarajan R, Nagarajaram HA, Ramakrishnan C. A procedure for the prediction of temperature-sensitive mutants of a globular protein based solely on the amino acid sequence. Proc Natl Acad Sci USA 1996; 93:13908-13.
14. Rennell D, Bouvier SE, Hardy LW, Poteete AR. Systematic mutation of bacteriophage T4 lysozyme. J Mol Biol 1991; 222:67-88.
15. Sandberg WS, Schlunk PM, Zabin HB, Terwilliger TC. Relationship between in vivo activity and in vitro measures of function and stability of a protein. Biochemistry 1995; 34:11970-8.
16. Chakshusmathi G, Mondal K, Lakshmi GS, Singh G, Roy A, Ch RB, Madhusudhanan S, Varadarajan R. Design of temperature-sensitive mutants solely from amino acid sequence. Proc Natl Acad Sci USA 2004; 101:7925-30.
17. Mondal K, Dastidar AG, Singh G, Madhusudhanan S, Gande SL, VijayRaghavan K, Varadarajan R. Design and isolation of temperature-sensitive mutants of Gal4 in yeast and Drosophila. J Mol Biol 2007; 370:939-50.
18. Bajaj K, Chakrabarti P, Varadarajan R. Mutagenesis-based definitions and probes of residue burial in proteins. Proc Natl Acad Sci USA 2005; 102:16221-6.
19. Bram RJ, Kornberg RD. Specific protein binding to far upstream activating sequences in polymerase II promoters. Proc Natl Acad Sci USA 1985; 82:43-7.
20. Fedor MJ, Lue NF, Kornberg RD. Statistical positioning of nucleosomes by specific protein-binding to an upstream activating sequence in yeast. J Mol Biol 1988; 204:109-27.
21. Giniger E, Varnum SM, Ptashne M. Specific DNA binding of GAL4, a positive regulatory protein of yeast. Cell 1985; 40:767-74.
22. Fischer JA, Giniger E, Maniatis T, Ptashne M. GAL4 activates transcription in Drosophila. Nature 1988; 332:853-6.
23. Kakidani H, Ptashne M. GAL4 activates gene expression in mammalian cells. Cell 1988; 52:161-7.
24. Ornitz DM, Moreadith RW, Leder P. Binary system for regulating transgene expression in mice: Targeting int-2 gene expression with yeast GAL4/UAS control elements. Proc Natl Acad Sci USA 1991; 88:698-702.
25. Andrulis ED, Neiman AM, Zappulla DC, Sternglanz R. Perinuclear localization of chromatin facilitates transcriptional silencing. Nature 1998; 394:592-5.
26. Rahner A, Scholer A, Martens E, Gollwitzer B, Schuller HJ. Dual influence of the yeast Cat1p (Snf1p) protein kinase on carbon source-dependent transcriptional activation of gluconeogenic genes by the regulatory gene CAT8. Nucleic Acids Res 1996; 24:2331-7.
27. Brand AH, Perrimon N. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 1993; 118:401-15.
28. Hartley KO, Nutt SL, Amaya E. Targeted gene expression in transgenic Xenopus using the binary Gal4-UAS system. Proc Natl Acad Sci USA 2002; 99:1377-82.
29. Scheer N, Camnos-Ortega JA. Use of the Gal4-UAS technique for targeted gene expression in the zebrafish. Mech Dev 1999; 80:153-8.
30. Marmorstein R, Carey M, Ptashne M, Harrison SC. DNA recognition by GAL4: Structure of a protein-DNA complex. Nature 1992; 356:408-14.
31. Wu Y, Reece RJ, Ptashne M. Quantitation of putative activator-target affinities predicts transcriptional activating potentials. Embo J 1996; 15:3951-63.
32. Sturtevant JM, Yu MH, Haase-Pettingell C, King J. Thermostability of temperature-sensitive folding mutants of the P22 tailspike protein. J Biol Chem 1989; 264:10693-8.
33. Chakrabarti A, Srivastava S, Swaminathan CP, Surolia A, Varadarajan R. Thermodynamics of replacing an alpha-helical Pro residue in the P40S mutant of Escherichia coli thioredoxin. Protein Sci 1999; 8:2455-9.