Biochemical analysis of a multifunctional cytochrome P450 (CYP51) enzyme required for synthesis of antimicrobial triterpenes in plants

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 35, 2013, Pages

  Geisler, Katrin ^a,b   Hughes, Richard K ^c   Sainsbury, Frank ^c,f   Lomonossoff, George P ^c   Rejzek, Martin ^c   Fairhurst, Shirley ^c   Olsen, Carl Erik ^b   Motawia, Mohammed Saddik ^b   Melton, Rachel E ^a   Hemmings, Andrew M ^d,e   Bak, Søren ^b   Osbourn, Anne ^a  

^a Department of Metabolic Biology ^*  (United Kingdom)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c JOHN INNES CENTRE   (United Kingdom)

^d Manchester Institute of Biotechnology   (United Kingdom)

^e UNIVERSITY OF EAST ANGLIA   (United Kingdom)

^f UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

CPMV HT transient expression; Cytochrome P450 monooxygenase CYP51 family; Disease resistance; Neofunctionalization; Terpenes

Indexed keywords

BETA AMYRIN; CYTOCHROME P450; PENTACYCLIC TRITERPENE; TRITERPENE; 2,3-OXIDOSQUALENE-BETA-AMYRIN-CYCLASE; ANTIINFECTIVE AGENT; MUTASE; STEROL 14ALPHA DEMETHYLASE;

ANTIFUNGAL ACTIVITY; ARTICLE; BIOASSAY; CHEMICAL ANALYSIS; EPOXIDATION; GENE EXPRESSION SYSTEM; HYDROXYLATION; MOLECULAR MODEL; NICOTIANA BENTHAMIANA; NONHUMAN; PLANT; PRIORITY JOURNAL; SYNTHESIS; AMINO ACID SEQUENCE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; OAT; SEQUENCE HOMOLOGY; TOBACCO;

AMINO ACID SEQUENCE; ANTI-INFECTIVE AGENTS; AVENA SATIVA; INTRAMOLECULAR TRANSFERASES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; SEQUENCE HOMOLOGY, AMINO ACID; STEROL 14-DEMETHYLASE; TOBACCO; TRITERPENES;

EID: 84883335229     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1309157110     Document Type: Article

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