Utilization of TREC and KREC quantification for the monitoring of early T- and B-cell neogenesis in adult patients after allogeneic hematopoietic stem cell transplantation

Journal of Translational Medicine

Volumn 11, Issue 1, 2013, Pages

  Mensen, Angela ^a,b   Ochs, Christoph ^a   Stroux, Andrea ^a   Wittenbecher, Friedrich ^a   Szyska, Martin ^b   Imberti, Luisa ^c   Fillatreau, Simon ^d   Uharek, Lutz ^a   Arnold, Renate ^a   Dörken, Bernd ^a   Thiel, Andreas ^a   Scheibenbogen, Carmen ^a   Na, Il Kang ^a,b  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b Experimental and Clinical Research Center ECRC   (Germany)

^c Laboratorio Interdipartimentale di Biologia Cellulare e Radio Biologia   (Italy)

^d Leibniz Institute   (Germany)

Author keywords

Acute leukemia; Allogeneic hematopoietic stem cell transplantation; Monitoring immune reconstitution; Simultaneous TREC KREC quantification assay

Indexed keywords

CD19 ANTIGEN; CD27 ANTIGEN; CD3 ANTIGEN; CD31 ANTIGEN; CD38 ANTIGEN; CD4 ANTIGEN; CD45RA ANTIGEN; CD45RO ANTIGEN; CHEMOKINE RECEPTOR CCR7; CYCLOSPORIN A; GENOMIC DNA; IMMUNOGLOBULIN D; INTERLEUKIN 7 RECEPTOR; L SELECTIN; METHOTREXATE; MYCOPHENOLIC ACID 2 MORPHOLINOETHYL ESTER; THYMOCYTE ANTIBODY;

ACUTE GRAFT VERSUS HOST DISEASE; ACUTE GRANULOCYTIC LEUKEMIA; ACUTE LYMPHOBLASTIC LEUKEMIA; ADULT; AGED; ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION; ARTICLE; B LYMPHOCYTE; BONE MARROW; CANCER SURVIVAL; CELL EXPANSION; CHEMOPROPHYLAXIS; CHRONIC GRAFT VERSUS HOST DISEASE; CLINICAL ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; DNA STRUCTURE; FEMALE; FLOW CYTOMETRY; GENE DOSAGE; HUMAN; HUMAN CELL; IMMUNE CELL RECONSTITUTION; IMMUNOLOGICAL MONITORING; IMMUNOLOGICAL PARAMETERS; KAPPA DELETING RECOMBINATION EXCISION CIRCLE; LIMIT OF DETECTION; LYMPHOCYTE COUNT; LYMPHOCYTOPOIESIS; MALE; MEMORY CELL; MEMORY T LYMPHOCYTE; OVERALL SURVIVAL; PERIPHERAL BLOOD MONONUCLEAR CELL; PHENOTYPE; REAL TIME POLYMERASE CHAIN REACTION; T CELL RECEPTOR EXCISION CIRCLE; T LYMPHOCYTE; T LYMPHOCYTE SUBPOPULATION; THYMUS;

ADULT; B-LYMPHOCYTES; DNA, CIRCULAR; FEMALE; FLOW CYTOMETRY; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HUMANS; MALE; MIDDLE AGED; RECEPTORS, ANTIGEN, T-CELL; T-LYMPHOCYTE SUBSETS; T-LYMPHOCYTES; TRANSPLANTATION, HOMOLOGOUS;

EID: 84883210876     PISSN: None     EISSN: 14795876     Source Type: Journal    
DOI: 10.1186/1479-5876-11-188     Document Type: Article

References (34)

1. Copelan EA. Hematopoietic stem-cell transplantation. N Engl J Med 2006, 354:1813-1826. 10.1056/NEJMra052638, 16641398.
2. Storek J, Geddes M, Khan F, Huard B, Helg C, Chalandon Y, Passweg J, Roosnek E. Reconstitution of the immune system after hematopoietic stem cell transplantation in humans. Semin Immunopathol 2008, 30:425-437. 10.1007/s00281-008-0132-5, 18949477.
3. Bemark M, Holmqvist J, Abrahamsson J, Mellgren K. Translational mini-review series on B cell subsets in disease. Reconstitution after haematopoietic stem cell transplantation - revelation of B cell developmental pathways and lineage phenotypes. Clin Exp Immunol 2012, 167:15-25. 10.1111/j.1365-2249.2011.04469.x, 3248082, 22132880.
4. Storek J, Wells D, Dawson MA, Storer B, Maloney DG. Factors influencing B lymphopoiesis after allogeneic hematopoietic cell transplantation. Blood 2001, 98:489-491. 10.1182/blood.V98.2.489, 11435323.
5. Na IK, Lu SX, Yim NL, Goldberg GL, Tsai J, Rao U, Smith OM, King CG, Suh D, Hirschhorn-Cymerman D, et al. The cytolytic molecules Fas ligand and TRAIL are required for murine thymic graft-versus-host disease. J Clin Invest 2010, 120:343-356. 10.1172/JCI39395, 2798682, 19955659.
6. Mackall CL, Bare CV, Granger LA, Sharrow SO, Titus JA, Gress RE. Thymic-independent T cell regeneration occurs via antigen-driven expansion of peripheral T cells resulting in a repertoire that is limited in diversity and prone to skewing. J Immunol 1996, 156:4609-4616.
7. Weinberg K, Blazar BR, Wagner JE, Agura E, Hill BJ, Smogorzewska M, Koup RA, Betts MR, Collins RH, Douek DC. Factors affecting thymic function after allogeneic hematopoietic stem cell transplantation. Blood 2001, 97:1458-1466. 10.1182/blood.V97.5.1458, 11222394.
8. Storek J, Dawson MA, Maloney DG. Correlation between the numbers of naive T cells infused with blood stem cell allografts and the counts of naive T cells after transplantation. Biol Blood Marrow Transplant 2003, 9:781-784. 10.1016/j.bbmt.2003.09.003, 14677118.
9. Storek J, Dawson MA, Storer B, Stevens-Ayers T, Maloney DG, Marr KA, Witherspoon RP, Bensinger W, Flowers ME, Martin P, et al. Immune reconstitution after allogeneic marrow transplantation compared with blood stem cell transplantation. Blood 2001, 97:3380-3389. 10.1182/blood.V97.11.3380, 11369627.
10. Arstila TP, Casrouge A, Baron V, Even J, Kanellopoulos J, Kourilsky P. A direct estimate of the human alphabeta T cell receptor diversity. Science 1999, 286:958-961. 10.1126/science.286.5441.958, 10542151.
11. Lausen BF, Hougs L, Schejbel L, Heilmann C, Barington T. Human memory B cells transferred by allogenic bone marrow transplantation contribute significantly to the antibody repertoire of the recipient. J Immunol 2004, 172:3305-3318.
12. Douek DC, McFarland RD, Keiser PH, Gage EA, Massey JM, Haynes BF, Polis MA, Haase AT, Feinberg MB, Sullivan JL, et al. Changes in thymic function with age and during the treatment of HIV infection. Nature 1998, 396:690-695. 10.1038/25374, 9872319.
13. Sottini A, Ghidini C, Zanotti C, Chiarini M, Caimi L, Lanfranchi A, Moratto D, Porta F, Imberti L. Simultaneous quantification of recent thymic T-cell and bone marrow B-cell emigrants in patients with primary immunodeficiency undergone to stem cell transplantation. Clin Immunol 2010, 136:217-227. 10.1016/j.clim.2010.04.005, 20452829.
14. Serana F, Airo P, Chiarini M, Zanotti C, Scarsi M, Frassi M, Lougaris V, Plebani A, Caimi L, Imberti L. Thymic and bone marrow output in patients with common variable immunodeficiency. J Clin Immunol 2011, 31:540-549. 10.1007/s10875-011-9526-6, 21491094.
15. Hazenberg MD, Otto SA, de Pauw ES, Roelofs H, Fibbe WE, Hamann D, Miedema F. T-cell receptor excision circle and T-cell dynamics after allogeneic stem cell transplantation are related to clinical events. Blood 2002, 99:3449-3453. 10.1182/blood.V99.9.3449, 11964316.
16. Borghans JA, Bredius RG, Hazenberg MD, Roelofs H, Jol-van der Zijde EC, Heidt J, Otto SA, Kuijpers TW, Fibbe WE, Vossen JM, et al. Early determinants of long-term T-cell reconstitution after hematopoietic stem cell transplantation for severe combined immunodeficiency. Blood 2006, 108:763-769. 10.1182/blood-2006-01-009241, 16822903.
17. Junge S, Kloeckener-Gruissem B, Zufferey R, Keisker A, Salgo B, Fauchere JC, Scherer F, Shalaby T, Grotzer M, Siler U, et al. Correlation between recent thymic emigrants and CD31+ (PECAM-1) CD4+ T cells in normal individuals during aging and in lymphopenic children. Eur J Immunol 2007, 37:3270-3280. 10.1002/eji.200636976, 17935071.
18. Serana F, Sottini A, Chiarini M, Zanotti C, Ghidini C, Lanfranchi A, Notarangelo LD, Caimi L, Imberti L. The different extent of B and T cell immune reconstitution after hematopoietic stem cell transplantation and enzyme replacement therapies in SCID patients with adenosine deaminase deficiency. J Immunol 2010, 185:7713-7722. 10.4049/jimmunol.1001770, 21057082.
19. Thiel A, Alexander T, Schmidt CA, Przybylski GK, Kimmig S, Kohler S, Radtke H, Gromnica-Ihle E, Massenkeil G, Radbruch A, et al. Direct assessment of thymic reactivation after autologous stem cell transplantation. Acta Haematol 2008, 119:22-27. 10.1159/000117824, 18292651.
20. Ribeiro RM, Perelson AS. Determining thymic output quantitatively: using models to interpret experimental T-cell receptor excision circle (TREC) data. Immunol Rev 2007, 216:21-34.
21. Douek DC, Vescio RA, Betts MR, Brenchley JM, Hill BJ, Zhang L, Berenson JR, Collins RH, Koup RA. Assessment of thymic output in adults after haematopoietic stem-cell transplantation and prediction of T-cell reconstitution. Lancet 2000, 355:1875-1881. 10.1016/S0140-6736(00)02293-5, 10866444.
22. Kohler S, Thiel A. Life after the thymus: CD31+ and CD31- human naive CD4+ T-cell subsets. Blood 2009, 113:769-774. 10.1182/blood-2008-02-139154, 18583570.
23. van Zelm MC, Szczepanski T, van der Burg M, van Dongen JJ. Replication history of B lymphocytes reveals homeostatic proliferation and extensive antigen-induced B cell expansion. J Exp Med 2007, 204:645-655. 10.1084/jem.20060964, 2137914, 17312005.
24. Fedele R, Martino M, Garreffa C, Messina G, Console G, Princi D, Dattola A, Moscato T, Massara E, Spiniello E, et al. The impact of early CD4+ lymphocyte recovery on the outcome of patients who undergo allogeneic bone marrow or peripheral blood stem cell transplantation. Blood Transfus 2012, 10:174-180. 3320776, 22337266.
25. Zeger SL, Liang KY. Longitudinal data analysis for discrete and continuous outcomes. Biometrics 1986, 42:121-130. 10.2307/2531248, 3719049.
26. Lorenzi AR, Patterson AM, Pratt A, Jefferson M, Chapman CE, Ponchel F, Isaacs JD. Determination of thymic function directly from peripheral blood: a validated modification to an established method. J Immunol Methods 2008, 339:185-194. 10.1016/j.jim.2008.09.013, 2593795, 18854192.
27. Haines CJ, Giffon TD, Lu LS, Lu X, Tessier-Lavigne M, Ross DT, Lewis DB. Human CD4+ T cell recent thymic emigrants are identified by protein tyrosine kinase 7 and have reduced immune function. J Exp Med 2009, 206:275-285. 10.1084/jem.20080996, 2646563, 19171767.
28. Yamanaka K, Yawalkar N, Jones DA, Hurwitz D, Ferenczi K, Eapen S, Kupper TS. Decreased T-cell receptor excision circles in cutaneous T-cell lymphoma. Clin Cancer Res 2005, 11:5748-5755. 10.1158/1078-0432.CCR-04-2514, 16115912.
29. Fry TJ, Mackall CL. The many faces of IL-7: from lymphopoiesis to peripheral T cell maintenance. J Immunol 2005, 174:6571-6576.
30. Fry TJ, Mackall CL. Interleukin-7: master regulator of peripheral T-cell homeostasis?. Trends Immunol 2001, 22:564-571. 10.1016/S1471-4906(01)02028-2, 11574281.
31. Carcelain G, Saint-Mezard P, Altes HK, Tubiana R, Grenot P, Rabian C, de Boer R, Costagliola D, Katlama C, Debre P, Autran B. IL-2 therapy and thymic production of naive CD4 T cells in HIV-infected patients with severe CD4 lymphopenia. AIDS 2003, 17:841-850. 10.1097/00002030-200304110-00009, 12660531.
32. Dion ML, Poulin JF, Bordi R, Sylvestre M, Corsini R, Kettaf N, Dalloul A, Boulassel MR, Debre P, Routy JP, et al. HIV infection rapidly induces and maintains a substantial suppression of thymocyte proliferation. Immunity 2004, 21:757-768. 10.1016/j.immuni.2004.10.013, 15589165.
33. Prebet T, Lhoumeau AC, Arnoulet C, Aulas A, Marchetto S, Audebert S, Puppo F, Chabannon C, Sainty D, Santoni MJ, et al. The cell polarity PTK7 receptor acts as a modulator of the chemotherapeutic response in acute myeloid leukemia and impairs clinical outcome. Blood 2010, 116:2315-2323. 10.1182/blood-2010-01-262352, 20558616.
34. Ferrando-Martinez S, Franco JM, Ruiz-Mateos E, Hernandez A, Ordonez A, Gutierrez E, Leal M. A reliable and simplified sj/beta-TREC ratio quantification method for human thymic output measurement. J Immunol Methods 2010, 352:111-117. 10.1016/j.jim.2009.11.007, 19919841.