DNA-PKcs Inhibition Sensitizes Cancer Cells to Carbon-Ion Irradiation via Telomere Capping Disruption

PLoS ONE

Volumn 8, Issue 8, 2013, Pages

  Zhou, Xin ^a,b,c   Zhang, Xin ^a,b,c   Xie, Yi ^a,b,c   Tanaka, Kaoru ^d   Wang, Bing ^d   Zhang, Hong ^a,b,c  

^a INSTITUTE OF MODERN PHYSICS   (China)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^c Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province   (China)

^d NATIONAL INSTITUTE OF RADIOLOGICAL SCIENCES   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN KINASE C; PROTEIN P21; PROTEIN P53;

APOPTOSIS; ARTICLE; CANCER CELL; CANCER CELL CULTURE; CANCER RADIOTHERAPY; CATALYSIS; CELL AGING; CELL SURVIVAL; CLONOGENIC ASSAY; CONTROLLED STUDY; CYTOTOXICITY TEST; DNA DAMAGE; DNA END JOINING REPAIR; DOUBLE STRANDED DNA BREAK; ENZYME ACTIVITY; ENZYME INHIBITION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IONIZING RADIATION; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; RADIOSENSITIVITY; REAL TIME POLYMERASE CHAIN REACTION; RNA CAPPING; TELOMERE; WESTERN BLOTTING;

DNA DAMAGE; DNA END-JOINING REPAIR; DNA-ACTIVATED PROTEIN KINASE; HELA CELLS; HUMANS; NUCLEAR PROTEINS; PROTEIN KINASE INHIBITORS; RADIATION, IONIZING; TELOMERE; TIME FACTORS; TUMOR SUPPRESSOR PROTEIN P53;

EID: 84883192431     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0072641     Document Type: Article

References (26)

1. Deng W, Tsao SW, Guan XY, Lucas JN, Si HX, et al. (2004) Distinct profiles of critically short telomeres are a key determinant of different chromosome aberrations in immortalized human cells: whole-genome evidence from multiple cell lines. Oncogene 23: 9090-9101.
2. AS IJ, Greider CW, (2003) Short telomeres induce a DNA damage response in Saccharomyces cerevisiae. Mol Biol Cell 14: 987-1001.
3. Zaal A, Louwers J, Berkhof J, Kocken M, Ter Harmsel W, et al. (2012) Agreement between colposcopic impression and histological diagnosis among human papillomavirus type 16-positive women: a clinical trial using dynamic spectral imaging colposcopy. BJOG.
4. Hou YY, Toh MT, Wang X, (2012) NBS1 deficiency promotes genome instability by affecting DNA damage signaling pathway and impairing telomere integrity. Cell Biochem Funct 30: 233-242.
5. Zhang Y, Zhou J, Lim CU, (2006) The role of NBS1 in DNA double strand break repair, telomere stability, and cell cycle checkpoint control. Cell Res 16: 45-54.
6. Kiecolt-Glaser JK, Glaser R, (2010) Psychological stress, telomeres, and telomerase. Brain Behav Immun 24: 529-530.
7. Daubenmier J, Lin J, Blackburn E, Hecht FM, Kristeller J, et al. (2012) Changes in stress, eating, and metabolic factors are related to changes in telomerase activity in a randomized mindfulness intervention pilot study. Psychoneuroendocrinology 37: 917-928.
8. Dewar JM, Lydall D, (2012) Similarities and differences between "uncapped" telomeres and DNA double-strand breaks. Chromosoma 121: 117-130.
9. von Zglinicki T, Pilger R, Sitte N, (2000) Accumulation of single-strand breaks is the major cause of telomere shortening in human fibroblasts. Free Radic Biol Med 28: 64-74.
10. Cawthon RM, (2009) Telomere length measurement by a novel monochrome multiplex quantitative PCR method. Nucleic Acids Res 37: e21.
11. Kantor AF, Li FP, Janov AJ, Tarbell NJ, Sallan SE, (1989) Hypertension in long-term survivors of childhood renal cancers. J Clin Oncol 7: 912-915.
12. Ward JF, (1981) Some Biochemical Consequences of the Spatial-Distribution of Ionizing Radiation-Produced Free-Radicals. Radiat Res 86: 185-195.
13. Ward JF, (1990) The Yield of DNA Double-Strand Breaks Produced Intracellularly by Ionizing-Radiation- a Review. Int J Radiat Biol 57: 1141-1150.
14. Lieber MR, (2008) The mechanism of human nonhomologous DNA end joining. Journal of Biological Chemistry 283: 1-5.
15. Mahaney BL, Meek K, Lees-Miller SP, (2009) Repair of ionizing radiation-induced DNA double-strand breaks by non-homologous end-joining. Biochemical Journal 417: 639-650.
16. Weterings E, Chen DJ, (2008) The endless tale of non-homologous end-joining. Cell Research 18: 114-124.
17. Li X, Heyer WD, (2008) Homologous recombination in DNA repair and DNA damage tolerance. Cell Research 18: 99-113.
18. Pang DL, Winters TA, Jung M, Purkayastha S, Cavalli LR, et al. (2011) Radiation-generated Short DNA Fragments May Perturb Non-homologous End-joining and Induce Genomic Instability. J Radiat Res 52: 309-319.
19. Jeggo PA, Geuting V, Lobrich M, (2011) The role of homologous recombination in radiation-induced double-strand break repair. Radiotherapy and Oncology 101: 7-12.
20. Drissi R, Wu J, Hu Y, Bockhold C, Dome JS, (2011) Telomere shortening alters the kinetics of the DNA damage response after ionizing radiation in human cells. Cancer Prev Res (Phila) 4: 1973-1981.
21. Sabatino L, Picano E, Andreassi MG, (2012) Telomere shortening and ionizing radiation: a possible role in vascular dysfunction? Int J Radiat Biol 88: 830-839.
22. Kusumoto-Matsuo R, Opresko PL, Ramsden D, Tahara H, Bohr VA, (2010) Cooperation of DNA-PKcs and WRN helicase in the maintenance of telomeric D-loops. Aging (Albany NY) 2: 274-284.
23. Espejel S, Franco S, Sgura A, Gae D, Bailey SM, et al. (2002) Functional interaction between DNA-PKcs and telomerase in telomere length maintenance. Embo Journal 21: 6275-6287.
24. Williams ES, Klingler R, Ponnaiya B, Hardt T, Schrock E, et al. (2009) Telomere dysfunction and DNA-PKcs deficiency: characterization and consequence. Cancer Res 69: 2100-2107.
25. Bailey SM, Meyne J, Chen DJ, Kurimasa A, Li GC, et al. (1999) DNA double-strand break repair proteins are required to cap the ends of mammalian chromosomes. Proc Natl Acad Sci U S A 96: 14899-14904.
26. Gilley D, Tanaka H, Hande MP, Kurimasa A, Li GC, et al. (2001) DNA-PKcs is critical for telomere capping. Proc Natl Acad Sci U S A 98: 15084-15088.