Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery

Molecules

Volumn 18, Issue 8, 2013, Pages 9999-10013

  Molnár, Eszter ^a,b   Kuntam, Soujanya ^a   Cingaram, Pradeep Kumar Reddy ^a   Peksel, Begüm ^a   Suresh, Bhavyashree ^a   Fábián, Gabriella ^b   Fehér, Liliána Z ^c   Bokros, Attila ^b   Medgyesi, Ágnes ^b   Ayaydin, Ferhan ^a   Puskás, László G ^a,b,c  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^b Avicor Ltd   (Hungary)

^c AVIDIN LTD   (Hungary)

Author keywords

Confocal laser scanning microscopy; Fluorescent dye discovery; High throughput screening; Small molecule microarray

Indexed keywords

FLUORESCENT DYE;

ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; CONFOCAL MICROSCOPY; METHODOLOGY; PROCEDURES;

FLUORESCENT DYES; MICROSCOPY, CONFOCAL; MOLECULAR STRUCTURE;

EID: 84883160072     PISSN: None     EISSN: 14203049     Source Type: Journal    
DOI: 10.3390/molecules18089999     Document Type: Article

References (23)

1. Tsien, R. Building and breeding molecules to spy on cells and tumors. FEBS Lett. 2005, 579, 927-932.
2. Weissleder, R.; Pittet, M. Imaging in the era of molecular oncology. Nature 2008, 452, 580-589.
3. Giepmans, B.N.G.; Adams, S.R.; Ellisman, M.H.; Tsien, R.Y. The Fluorescent Toolbox for Assessing Protein Location and Function. Science 2006, 312, 217-224.
4. Henrich, S.; Salo-Ahen, O.M.H.; Huang, B.; Rippmann, F.F.; Cruciani, G.; Wade, R.C. Computational approaches to identifying and characterizing protein binding sites for ligand design. J. Mol. Recognit. 2010, 23, 209-219.
5. Manning, H.C.; Lander, A.; McKinley, E.; Mutic, N.J. Accelerating the development of novel molecular imaging probes: A role for high-throughput screening. J. Nucl. Med. 2008, 49, 1401-1404.
6. Simeonov, A.; Jadhav, A.; Thomas, C.J.; Wang, Y.; Huang, R.; Southall, N.T.; Shinn, P.; Smith, J.; Austin, C.P.; Auld, D.S.; et al. Fluorescence spectroscopic profiling of compound libraries. J. Med. Chem. 2008, 51, 2363-2371.
7. Lea, W.A.; Simeonov, A. Fluorescence polarization assays in small molecule screening. Expert Opin. Drug. Discov. 2011, 6, 17-32.
8. Yun, S.W.; Leong, C.; Zhai, D.; Tan, Y.L.; Lim, L.; Bi, X.; Lee, J.J.; Kim, H.J.; Kang, N.Y.; Ng, S.H.; et al. Neural stem cell specific fluorescent chemical probe binding to FABP7. P. Natl. Acad. Sci. USA 2012, 109, 10214-10217.
9. Li, Q.; Kim, Y.; Namm, J.; Kulkarni, A.; Rosania, G.R.; Ahn, Y.H.; Chang, Y.T. RNA-selective, live cell imaging probes for studying nuclear structure and function. Chem. Biol. 2006, 13, 615-623.
10. Hackler, L., Jr.; Dorman, G.; Kele, Z.; Urge, L.; Darvas, F.; Puskas, L.G. Development of chemically modified glass surfaces for nucleic acid, protein and small molecule microarrays. Mol. Divers. 2003, 7, 25-36.
11. Darvas, F.; Dorman, G.; Krajcsi, P.; Puskas, L.G.; Kovari, Z.; Lorincz, Z.; Urge, L. Recent advances in chemical genomics. Curr. Med. Chem. 2004, 11, 3119-3145.
12. Molnár, E.; Hackler, L.; Jankovics, T.; Ürge, L.; Darvas, F.; Fehér, L.Z.; Lorincz, Z.; Dormán, G.; Puskás, L.G. Application of small molecule microarrays in comparative chemical proteomics. QSAR Comb. Sci. 2006, 25, 1020-1026.
13. Chen, J.; Armstrong, A.H.; Koehler, A.N.; Hecht, M.H. Small molecule microarrays enable the discovery of compounds that bind the Alzheimer's Abeta peptide and reduce its cytotoxicity. J. Am. Chem. Soc. 2010, 132, 17015-17022.
14. Lee, H.Y.; Park, S.B. Surface modification for small-molecule microarrays and its application to the discovery of a tyrosinase inhibitor. Mol. Biosyst. 2011, 7, 304-310.
15. Shi, H.; Liu, K.; Xu, A.; Yao, S.Q. Small molecule microarray-facilitated screening of affinity-based probes (AfBPs) for gamma-secretase. Chem. Commun. 2009, 5030-5032.
16. Vegas, A.J.; Bradner, J.E.; Tang, W.; McPherson, O.M.; Greenberg, E.F.; Koehler, A.N.; Schreiber, S.L. Fluorous-based small-molecule microarrays for the discovery of histone deacetylase inhibitors. Angew. Chem. Int. Ed. 2007, 46, 7960-7964.
17. Barnes-Seeman, D.; Park, S.B.; Koehler, A.N.; Schreiber, S.L. Expanding the functional group compatibility of small-molecule microarrays: Discovery of novel calmodulin ligands. Angew. Chem. Int. Edit. 2003, 42, 2376-2379.
18. Oheim, M. Advances and challenges in high-throughput microscopy for live-cell subcellular imaging. Expert Opin. Drug. Dis. 2011, 6, 1299-1315.
19. Haugland, R.P.; Spence, M.T.Z.; Johnson, I.D.; Basey, A. The Handbook: A Guide to Fluorescent Probes and Labeling Technologies, 10th ed.; Molecular Probes: Eugene, OR, USA, 2005.
20. Puskas, L.G.; Feher, L.Z.; Vizler, C.; Ayaydin, F.; Raso, E.; Molnar, E.; Magyary, I.; Kanizsai, I.; Gyuris, M.; Madacsi, R.; et al. Polyunsaturated fatty acids synergize with lipid droplet binding thalidomide analogs to induce oxidative stress in cancer cells. Lipids Health Dis. 2010, 9, 56.
21. Marín, M.L.; Miguel, A.; Santos-Juanes, L.; Arques, A.; Amat, A.M.; Miranda, M.A. Involvement of triplet excited states in the electron transfer photodegradation of cinnamic acids using pyrylium and thiapyrylium salts as photocatalysts. Photochem. Photobiol. Sci. 2007, 8, 848-852.
22. Alvarez, M.; Villanueva, A.; Acedo, P.; Cañete, M.; Stockert, J.C. Cell death causes relocalization of photosensitizing fluorescent probes. Acta Histochem. 2011, 113, 363-368.
23. Johnson, V.L.; Ko, S.C.; Holmstrom, T.H.; Eriksson, J.E.; Chow, S.C. Effector caspases are dispensable for the early nuclear morphological changes during chemical-induced apoptosis. J. Cell Sci. 2000, 113, 2941-2953.