Inhibition of cytoplasmic streaming by cytochalasin D is superior to paraformaldehyde fixation for measuring FRET between fluorescent protein-tagged Golgi components

Cytometry Part A

Volumn 83, Issue 9, 2013, Pages 830-838

  Poulsen, Christian Peter ^a   Vereb, György ^b   Geshi, Naomi ^a   Schulz, Alexander ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b UNIVERSITY OF DEBRECEN   (Hungary)

Author keywords

Cytochalasin D; Cytoplasmic streaming; Fixation; Fluorescent proteins; FRET; Glycosyltransferase; Golgi; Nicotiana benthamiana; Paraformaldehyde; Plants; Spectral imaging

Indexed keywords

BACTERIAL PROTEIN; CYAN FLUORESCENT PROTEIN; CYTOCHALASIN D; FORMALDEHYDE; GLYCOSYLTRANSFERASE; GREEN FLUORESCENT PROTEIN; PARAFORMALDEHYDE; PHOTOPROTEIN; POLYMER; YELLOW FLUORESCENT PROTEIN, BACTERIA;

AGROBACTERIUM; ARTICLE; CELL MOTION; CHEMISTRY; CONFOCAL MICROSCOPY; DRUG EFFECT; FIXATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT PROTEINS; FRET; GENETICS; GOLGI; GOLGI COMPLEX; METABOLISM; METHODOLOGY; NICOTIANA BENTHAMIANA; PLANT; PROTEIN ANALYSIS; PROTEIN BINDING; SPECTRAL IMAGING; TOBACCO;

CYTOCHALASIN D; CYTOPLASMIC STREAMING; FIXATION; FLUORESCENT PROTEINS; FRET; GLYCOSYLTRANSFERASE; GOLGI; NICOTIANA BENTHAMIANA; PARAFORMALDEHYDE; PLANTS; SPECTRAL IMAGING;

AGROBACTERIUM; BACTERIAL PROTEINS; CYTOCHALASIN D; CYTOPLASMIC STREAMING; FLUORESCENCE RESONANCE ENERGY TRANSFER; FORMALDEHYDE; GOLGI APPARATUS; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MICROSCOPY, CONFOCAL; POLYMERS; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; TOBACCO;

EID: 84883051647     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22282     Document Type: Article

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