Identification of CD68+ neutrophil granulocytes in in vitro model of acute inflammation and inflammatory bowel disease

International Journal of Clinical and Experimental Pathology

Volumn 6, Issue 4, 2013, Pages 561-570

  Amanzada, Ahmad ^a   Ahmed Malik, Ihtzaz ^a   Blaschke, Martina ^a   Khan, Sajjad ^a   Rahman, Hazir ^a   Ramadori, Giuliano ^a   Moriconi, Federico ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

CD 68; Inflammatory bowel disease; Monocytes; Neutrophil granulocytes; Peripheral blood mononuclear cells

Indexed keywords

BIOLOGICAL MARKER; CD163 ANTIGEN; CD68 ANTIGEN, HUMAN; CELL SURFACE RECEPTOR; DIFFERENTIATION ANTIGEN; LEUKOCYTE ANTIGEN; LEUKOCYTE ELASTASE; PEROXIDASE;

ARTICLE; BIOPSY; CASE CONTROL STUDY; CD-68; CELL CULTURE; COMPARATIVE STUDY; ENTERITIS; FEMALE; GRANULOCYTE; HUMAN; IN VITRO STUDY; INTESTINE MUCOSA; MACROPHAGE; MALE; METABOLISM; MONOCYTE; MONONUCLEAR CELL; NEUTROPHIL; NEUTROPHIL GRANULOCYTES; PATHOLOGY; PERIPHERAL BLOOD MONONUCLEAR CELLS;

CD-68; INFLAMMATORY BOWEL DISEASE; MONOCYTES; NEUTROPHIL GRANULOCYTES; PERIPHERAL BLOOD MONONUCLEAR CELLS;

ANTIGENS, CD; ANTIGENS, DIFFERENTIATION, MYELOMONOCYTIC; BIOLOGICAL MARKERS; BIOPSY; CASE-CONTROL STUDIES; CELLS, CULTURED; FEMALE; GRANULOCYTES; HUMANS; INFLAMMATORY BOWEL DISEASES; INTESTINAL MUCOSA; LEUKOCYTE ELASTASE; LEUKOCYTES, MONONUCLEAR; MACROPHAGES; MALE; MONOCYTES; NEUTROPHILS; PEROXIDASE; RECEPTORS, CELL SURFACE;

EID: 84882990539     PISSN: None     EISSN: 19362625     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Ionescu RM, Vlasak J, Price C and Kirchmeier M. Contribution of variable domains to the stability of humanized IgG1 monoclonal antibodies. J Pharm Sci 2008; 97: 1414-1426.
2. Davey FR, Cordell JL, Erber WN, Pulford KA, Gatter KC, Mason DY. Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes, tissue macrophages. J Clin Pathol 1988; 41: 753-758.
3. Kelly PM, Bliss E, Morton JA, Burns J, McGee JO. Monoclonal antibody EBM/11: high cellular specificity for human macrophages. J Clin Pathol 1988; 41: 510-515.
4. Kreipe H, Radzun HJ, Parwaresch MR, Haislip A, Hansmann ML. Ki-M7 monoclonal antibody specific for myelomonocytic cell lineage, macrophages in human. J Histochem Cytochem 1987; 35: 1117-1126.
5. Parwaresch MR, Radzun HJ, Kreipe H, Hansmann ML, Barth J. Monocyte/macrophage-reactive monoclonal antibody Ki-M6 recognizes an intracytoplasmic antigen. Am J Pathol 1986; 125: 141-151.
6. Pulford KA, Rigney EM, Micklem KJ, Jones M, Stross WP, Gatter KC, Mason DY. KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. J Clin Pathol 1989; 42: 414-421.
7. Micklem K, Rigney E, Cordell J, Simmons D, Stross P, Turley H, Seed B, Mason D. A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies. Br J Haematol 1989; 73: 6-11.
8. Matsumoto H, Kumon Y, Watanabe H, Ohnishi T, Shudou M, Ii C, Takahashi H, Imai Y, Tanaka J. Antibodies to CD11b, CD68,, lectin label neutrophils rather than microglia in traumatic, ischemic brain lesions. J Neurosci Res 2007; 85: 994-1009.
9. Strobl H, Scheinecker C, Csmarits B, Majdic O, Knapp W. Flow cytometric analysis of intracellular CD68 molecule expression in normal, malignant haemopoiesis. Br J Haematol 1995; 90: 774-782.
10. Umino T, Skold CM, Pirruccello SJ, Spurzem JR, Rennard SI. Two-colour flow-cytometric analysis of pulmonary alveolar macrophages from smokers. Eur Respir J 1999; 13: 894-899.
11. + cells of monocyte/macrophage lineage in the environment of AIDS-associated, classic-sporadic Kaposi sarcoma are singly or doubly infected with human herpesviruses 7, 6B. Proc Natl Acad Sci U S A 1997; 94: 7600-7605.
12. Hakkinen T, Karkola K, Yla-Herttuala S. Macrophages, smooth muscle cells, endothelial cells,, T-cells express CD40, CD40L in fatty streaks, more advanced human atherosclerotic lesions. Colocalization with epitopes of oxidized low-density lipoprotein, scavenger receptor,, CD16 (Fc gammaRIII). Virchows Arch 2000; 437: 396-405.
13. Kunisch E, Fuhrmann R, Roth A, Winter R, Lungershausen W, Kinne RW. Macrophage specificity of three anti-CD68 monoclonal antibodies (KP1, EBM11,, PGM1) widely used for immunohistochemistry, flow cytometry. Ann Rheum Dis 2004; 63: 774-784.
14. Gottfried E, Kunz-Schughart LA, Weber A, Rehli M, Peuker A, Muller A, Kastenberger M, Brockhoff G,, Andreesen R, Kreutz M. Expression of CD68 in non-myeloid cell types. ScJ Immunol 2008; 67: 453-463.
15. Hameed A, Hruban RH, Gage W, Pettis G, Fox WM III. Immunohistochemical expression of CD68 antigen in human peripheral blood T cells. Hum Pathol 1994; 25: 872-876.
16. Amanzada A, Malik IA, Nischwitz M, Sultan S, Naz N, Ramadori G. Myeloperoxidase, elastase are only expressed by neutrophils in normal, in inflamed liver. Histochem Cell Biol 2011; 135: 305-315.
17. Miyazaki K, Sakuma K, Kawamura YI, Izawa M, Ohmori K, Mitsuki M, Yamaji T, Hashimoto Y, Suzuki A, Saito Y, Dohi T, Kannagi R. Colonic epithelial cells express specific ligands for mucosal macrophage immunosuppressive receptors siglec-7, -9. J Immunol 2012; 188: 4690-4700.
18. Woywodt A, Ludwig D, Neustock P, Kruse A, Schwarting K, Jantschek G, Kirchner H, Stange EF. Mucosal cytokine expression, cellular markers, adhesion molecules in inflammatory bowel disease. Eur J Gastroenterol Hepatol 1999; 11: 267-276.
19. Bataille F, Klebl F, Rummele P, Schroeder J, Farkas S, Wild PJ, Furst A, Hofstadter F, Scholmerich J, Herfarth H, Rogler G. Morphological characterisation of Crohn's disease fistulae. Gut 2004; 53: 1314-1321.
20. Perminow G, Reikvam DH, Lyckander LG, Brandtzaeg P, Vatn MH, Carlsen HS. Increased number, activation of colonic macrophages in pediatric patients with untreated Crohn's disease. Inflamm Bowel Dis 2009; 15: 1368-1378.
21. Moriconi F, Malik IA, Amanzada A, Blaschke M, Raddatz D, Khan S, Ramadori G. The anti-TNF-alpha antibody infliximab indirectly regulates PECAM-1 gene expression in two models of in vitro blood cell activation. Lab Invest 2012; 92: 166-177.
22. Moriconi F, Raddatz D, Ho NA, Yeruva S, Dudas J, Ramadori G. Quantitative gene expression of cytokines in peripheral blood leukocytes stimulated in vitro: modulation by the anti-tumor nerosis factor-alpha antibody infliximab, comparison with the mucosal cytokine expression in patients with ulcerative colitis. Transl Res 2007; 150: 223-232.
23. Malik IA, Moriconi F, Sheikh N, Naz N, Khan S, Dudas J, Mansuroglu T, Hess CF, Rave-Frank M, Christiansen H, Ramadori G. Single-dose gamma-irradiation induces up-regulation of chemokine gene expression, recruitment of granulocytes into the portal area but not into other regions of rat hepatic tissue. Am J Pathol 2010; 176: 1801-1815.
24. Nahar-Gohad P, Sultan H, Esteban Y, Stabile A, Ko JL. RACK1 identified as the PCBP1-interacting protein with a novel functional role on the regulation of human MOR gene expression. J Neurochem 2013; 124: 466-477.
25. Gough PJ, Gordon S, Greaves DR. The use of human CD68 transcriptional regulatory sequences to direct high-level expression of class A scavenger receptor in macrophages in vitro, in vivo. Immunology 2001; 103: 351-361.
26. Warnke RA, Pulford KA, Pallesen G, Ralfkiaer E, Brown DC, Gatter KC, Mason DY. Diagnosis of myelomonocytic, macrophage neoplasms in routinely processed tissue biopsies with monoclonal antibody KP1. Am J Pathol 1989; 135: 1089-1095.
27. Hodge GL, Flower R, Han P. Optimal storage conditions for preserving granulocyte viability as monitored by Annexin V binding in whole blood. J Immunol Methods 1999; 225: 27-38.
28. Antoniades CG, Quaglia A, Taams LS, Mitry RR, Hussain M, Abeles R, Possamai LA, Bruce M, McPhail M, Starling C, Wagner B, Barnardo A, Pomplun S, Auzinger G, Bernal W, Heaton N, Vergani D, Thursz MR, Wendon J. Source, characterization of hepatic macrophages in acetaminophen-induced acute liver failure in humans. Hepatology 2012; 56: 735-746.
29. Tousson E, Beltagy DM, Gazia MA, Al-Behbehani B. Expressions of P53, CD68 in mouse liver with Schistosoma mansoni infection, the protective role of silymarin. Toxicol Ind Health 2012. [Epub ahead of print].
30. Miura K, Yang L, van RN, Ohnishi H, Seki E. Hepatic recruitment of macrophages promotes nonalcoholic steatohepatitis through CCR2. Am J Physiol Gastrointest Liver Physiol 2012; 302: G1310-G1321.