Expression of CD68 in non-myeloid cell types

Scandinavian Journal of Immunology

Volumn 67, Issue 5, 2008, Pages 453-463

  Gottfried, E ^a   Kunz Schughart, L A ^b   Weber, A ^c   Rehli, M ^a   Peuker, A ^a   Muller A ^a   Kastenberger, M ^a   Brockhoff, G ^a   Andreesen, R ^a   Kreutz, M ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^c UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; CD68 ANTIBODY; CD68 ANTIGEN; FORMALDEHYDE; MESSENGER RNA; MONOCLONAL ANTIBODY KP1; PARAFFIN; UNCLASSIFIED DRUG;

ARTICLE; CANCER CELL CULTURE; ENDOTHELIUM CELL; FIBROBLAST; FLOW CYTOMETRY; FROZEN SECTION; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; LYSOSOME; MACROPHAGE; MONOCYTE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STROMA CELL; TRANSCRIPTION INITIATION; TRANSCRIPTION INITIATION SITE; TUMOR CELL; WESTERN BLOTTING;

ANTIGENS, CD; ANTIGENS, DIFFERENTIATION, MYELOMONOCYTIC; BASE SEQUENCE; BIOLOGICAL MARKERS; BLOTTING, WESTERN; CELL LINE; DENDRITIC CELLS; FIBROBLASTS; FLOW CYTOMETRY; HUMANS; IMMUNOHISTOCHEMISTRY; LYMPHOCYTES; MOLECULAR SEQUENCE DATA; MONOCYTES; PROMOTER REGIONS (GENETICS); REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; TISSUE FIXATION; TRANSCRIPTION INITIATION SITE;

EID: 42049096396     PISSN: 03009475     EISSN: 13653083     Source Type: Journal    
DOI: 10.1111/j.1365-3083.2008.02091.x     Document Type: Article

References (36)

1. Saito N, Pulford KA, Breton-Gorius J, Masse JM, Mason DY, Cramer EM. Ultrastructural localization of the CD68 macrophage-associated antigen in human blood neutrophils and monocytes. Am J Pathol 1991 139 : 1053 9.
2. Holness CL, da Silva RP, Fawcett J, Gordon S, Simmons DL. Macrosialin, a mouse macrophage-restricted glycoprotein, is a member of the lamp/lgp family. J Biol Chem 1993 268 : 9661 6.
3. Holness CL, Simmons DL. Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. Blood 1993 81 : 1607 13.
4. Kurushima H, Ramprasad M, Kondratenko N, Foster DM, Quehenberger O, Steinberg D. Surface expression and rapid internalization of macrosialin (mouse CD68) on elicited mouse peritoneal macrophages. J Leukoc Biol 2000 67 : 104 8.
5. Ramprasad MP, Terpstra V, Kondratenko N, Quehenberger O, Steinberg D. Cell surface expression of mouse macrosialin and human CD68 and their role as macrophage receptors for oxidized low density lipoprotein. Proc Natl Acad Sci U S A 1996 93 : 14833 8.
6. Parwaresch MR, Radzun HJ, Kreipe H, Hansmann ML, Barth J. Monocyte/macrophage-reactive monoclonal antibody Ki-M6 recognizes an intracytoplasmic antigen. Am J Pathol 1986 125 : 141 51.
7. Kreipe H, Radzun HJ, Parwaresch MR, Haislip A, Hansmann ML. Ki-M7 monoclonal antibody specific for myelomonocytic cell lineage and macrophages in human. J Histochem Cytochem 1987 35 : 1117 26.
8. Davey FR, Cordell JL, Erber WN, Pulford KA, Gatter KC, Mason DY. Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages. J Clin Pathol 1988 41 : 753 8.
9. Kelly PM, Bliss E, Morton JA, Burns J, McGee JO. Monoclonal antibody EBM/11: high cellular specificity for human macrophages. J Clin Pathol 1988 41 : 510 5.
10. Pulford KA, Rigney EM, Micklem KJ et al. KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. J Clin Pathol 1989 42 : 414 21.
11. Radzun HJ, Hansmann ML, Heidebrecht HJ et al. Detection of a monocyte/macrophage differentiation antigen in routinely processed paraffin-embedded tissues by monoclonal antibody Ki-M1P. Lab Invest 1991 65 : 306 15.
12. Falini B, Flenghi L, Pileri S et al. PG-M1: a new monoclonal antibody directed against a fixative-resistant epitope on the macrophage-restricted form of the CD68 molecule. Am J Pathol 1993 142 : 1359 72.
13. Micklem K, Rigney E, Cordell J et al. A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies. Br J Haematol 1989 73 : 6 11.
14. Kempf W, Adams V, Wey N et al. CD68 + cells of monocyte/macrophage lineage in the environment of AIDS-associated and classic-sporadic Kaposi sarcoma are singly or doubly infected with human herpesviruses 7 and 6B. Proc Natl Acad Sci U S A 1997 94 : 7600 5.
15. Hakkinen T, Karkola K, Yla-Herttuala S. Macrophages, smooth muscle cells, endothelial cells, and T-cells express CD40 and CD40L in fatty streaks and more advanced human atherosclerotic lesions. Colocalization with epitopes of oxidized low-density lipoprotein, scavenger receptor, and CD16 (Fc gammaRIII). Virchows Arch 2000 437 : 396 405.
16. Soilleux EJ, Morris LS, Trowsdale J, Coleman N, Boyle JJ. Human atherosclerotic plaques express DC-SIGN, a novel protein found on dendritic cells and macrophages. J Pathol 2002 198 : 511 6.
17. Kunisch E, Fuhrmann R, Roth A et al. Macrophage specificity of three anti-CD68 monoclonal antibodies (KP1, EBM11, and PGM1) widely used for immunohistochemistry and flow cytometry. Ann Rheum Dis 2004 63 : 774 84.
18. Andreesen R, Brugger W, Scheibenbogen C et al. Surface phenotype analysis of human monocyte to macrophage maturation. J Leukoc Biol 1990 47 : 490 7.
19. Stevenson HC, Miller P, Akiyama Y et al. A system for obtaining large numbers of cryopreserved human monocytes purified by leukapheresis and counter-current centrifugation elutriation (CCE). J Immunol Methods 1983 62 : 353 63.
20. Andreesen R, Picht J, Lohr GW. Primary cultures of human blood-born macrophages grown on hydrophobic teflon membranes. J Immunol Methods 1983 56 : 295 304.
21. Kunz-Schughart LA, Heyder P, Schroeder J, Knuechel R. A heterologous 3-D coculture model of breast tumor cells and fibroblasts to study tumor-associated fibroblast differentiation. Exp Cell Res 2001 266 : 74 86.
22. Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 1973 52 : 2745 56.
23. Cordell JL, Falini B, Erber WN et al. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 1984 32 : 219 29.
24. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987 162 : 156 9.
25. Greaves DR, Quinn CM, Seldin MF, Gordon S. Functional comparison of the murine macrosialin and human CD68 promoters in macrophage and nonmacrophage cell lines. Genomics 1998 54 : 165 8.
26. Gough PJ, Gordon S, Greaves DR. The use of human CD68 transcriptional regulatory sequences to direct high-level expression of class A scavenger receptor in macrophages in vitro and in vivo. Immunology 2001 103 : 351 61.
27. O'Reilly D, Quinn CM, El Shanawany T, Gordon S, Greaves DR. Multiple Ets factors and interferon regulatory factor-4 modulate CD68 expression in a cell type-specific manner. J Biol Chem 2003 : 278 : 21909 19.
28. Pulford KA, Sipos A, Cordell JL, Stross WP, Mason DY. Distribution of the CD68 macrophage/myeloid associated antigen. Int Immunol 1990 2 : 973 80.
29. Kaiser U, Hansmann ML, Papadopoulos I, Radzun HJ. Monocyte/macrophage- directed antibodies Ki-M3 and Ki-M7 detect renal-cell carcinomas. Int J Cancer Suppl 1988 3 : 45 9.
30. Doussis IA, Gatter KC, Mason DY. CD68 reactivity of non-macrophage derived tumours in cytological specimens. J Clin Pathol 1993 46 : 334 6.
31. Gloghini A, Rizzo A, Zanette I et al. KP1/CD68 expression in malignant neoplasms including lymphomas, sarcomas, and carcinomas. Am J Clin Pathol 1995 103 : 425 31.
32. Facchetti F, Bertalot G, Grigolato PG. KP1 (CD 68) staining of malignant melanomas. Histopathology 1991 19 : 141 5.
33. Tos AP, Doglioni C, Laurino L, Fletcher CD. KP1 (CD68) expression in benign neural tumours. Further evidence of its low specificity as a histiocytic/myeloid marker. Histopathology 1993 23 : 185 7.
34. Cassidy M, Loftus B, Whelan A et al. KP-1: not a specific marker. Staining of 137 sarcomas, 48 lymphomas, 28 carcinomas, 7 malignant melanomas and 8 cystosarcoma phyllodes. Virchows Arch 1994 424 : 635 40.
35. Hasegawa T, Hirose T, Seki K, Sano T, Hizawa K. Transforming growth factor alpha and CD68 immunoreactivity in giant cell tumours of bone: a study on the nature of stromal and giant cells, and their interrelations. J Pathol 1993 170 : 305 10.
36. O'Reilly D, Greaves DR. Cell-type-specific expression of the human CD68 gene is associated with changes in Pol II phosphorylation and short-range intrachromosomal gene looping. Genomics 2007 90 : 407 15.