The effect of the microgravity rotating culture system on the chondrogenic differentiation of bone marrow mesenchymal stem cells

Molecular Biotechnology

Volumn 54, Issue 2, 2013, Pages 331-336

  Wu, Xing ^a   Li, Shao Hua ^a   Lou, Lie Ming ^a   Chen, Zheng Rong ^b  

^a TONGJI UNIVERSITY SCHOOL OF MEDICINE   (China)

^b FUDAN UNIVERSITY   (China)

Author keywords

Chondrogenic differentiation; Marrow mesenchymal stem cells (MSCs); Microgravity rotating culture system

Indexed keywords

BONE MARROW MESENCHYMAL STEM CELLS; BONE MARROW MESENCHYMAL STEM CELLS (MSCS); CARTILAGE EXTRACELLULAR MATRIXES; CHONDROGENIC DIFFERENTIATION; CULTURE SYSTEMS; MARROW MESENCHYMAL STEM CELLS; PROTEIN EXPRESSIONS; TISSUE ENGINEERED CARTILAGE;

CARTILAGE; COLLAGEN; FLOWCHARTING; GLYCOPROTEINS; MICROGRAVITY; MICROGRAVITY PROCESSING; STEM CELLS;

CELL CULTURE;

AGGRECAN; COLLAGEN TYPE 2; MESSENGER RNA; POLYGLYCOLIC ACID; PROTEOGLYCAN; TOLONIUM CHLORIDE;

ANIMAL CELL; ARTICLE; BONE MARROW; BONE MARROW MESENCHYMAL STEM CELL; CARTILAGE; CARTILAGE CELL; CELL DIFFERENTIATION; CHONDROGENIC DIFFERENTIATION; CONTROLLED STUDY; MESENCHYMAL STEM CELL; MICROGRAVITY; NONHUMAN; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; STAINING; TISSUE ENGINEERING; WESTERN BLOTTING;

AGGRECANS; ANIMALS; BONE MARROW CELLS; CARTILAGE; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELLS, CULTURED; CHONDROCYTES; CHONDROGENESIS; COLLAGEN TYPE II; EXTRACELLULAR MATRIX PROTEINS; MATRILIN PROTEINS; MESENCHYMAL STROMAL CELLS; POLYGLYCOLIC ACID; RABBITS; TISSUE ENGINEERING; WEIGHTLESSNESS;

EID: 84882868929     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-012-9568-x     Document Type: Article

References (24)

1. Tocci, A., & Forte, L. (2003). Mesenchymal stem cell: Use and perspectives. Hematology Journal, 4, 92-96.
2. Cancedda, R., Dozin, B., Giannoni, P., & Quarto, R. (2003). Tissue engineering and cell therapy of cartilage and bone. Matrix Biology, 22, 81-91.
3. Short, B., Brouard, N., Occhiodoro-Scott, T., Ramakrishnan, A., & Simmons, P. J. (2003). Mesenchymal stem cells. Archives of Medical Research, 34, 565-571.
4. Ishaug-Riley, S. L., Crane-Kruger, G. M., Yaszemski, M. J., & Mikos, A. G. (1998). Three-dimensional culture of rat calvarial osteoblasts in porous biodegradable polymers. Biomaterials, 19, 1405-1412.
5. Jessup, J. M., Frantz, M., Sonmez-Alpan, E., Locker, J., Skena, K., Waller, H., et al. (2000). Microgravity culture reduces apoptosis and increases the differentiation of a human colorectal carcinoma cell line. In Vitro Cellular & Developmental Biology - Animal, 36, 367-377.
6. Dexter, T. M., Allen, T. D., & Lajhta, L. G. (1977). Conditions controlling the proliferation of hematopoietic stem cells in vitro. Journal of Cellular Physiology, 91, 335-344.
7. Lee, Y. M., Park, Y. J., Lee, S. J., Ku, Y., Han, S. B., & Choi, S. M. (2000). Tissue engineered bone formation using chitosan/tricalcium phosphate sponges. Journal of Periodontology, 72, 410-417.
8. Meyers, V. E., Zayzafoon, M., Douglas, J. T., & McDonald, J. M. (2005). RhoA and cytoskeletal disruption mediate reduced osteoblastogenesis and enhanced adipogenesis of human mesenchymal stem cells in modeled microgravity. Journal of Bone and Mineral Research, 20, 1858-1866.
9. Sheyn, D., Pelled, G., Netanely, D., Domany, E., & Gazit, D. (2010). The effect of simulated microgravity on human mesenchymal stem cells cultured in an osteogenic differentiation system: A bioinformatics study. Tissue Engineering Part A, 16, 3403-3412.
10. Ishaug, S. L., Crane, G. M., Miller, M. J., Yasko, A. W., Yaszemski, M. J., & Mikos, A. G. (2005). Aggregation of pig articular chondrocyte and synthesis of extracellular matrix by a lactose-modified chitosan. Biomaterials, 26, 987-998.
11. Gran Laroche, N., & Vico, L. (1998). Rotating-well vessels, promising bioreactors for osteoblastic cell culture: Comparison with other 3D conditions. Medical & Biological Engineering & Computing, 36, 513-519.
12. Holy, C. E., Syhoichet, M. S., & Davis, J. E. (2000). Engineering three-dimensional bone tissue in vitro using biodegradable scaffolds: Investigating initial cell-seeding density and culture period. Journal of Biomedical Materials Research Part A, 51, 376-382.
13. Ishaug, S. L., Crane, G. M., Miller, M. J., Yasko, A. W., Yaszemski, M. J., & Mikos, A. G. (1997). Bone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffolds. Journal of Biomedical Materials Research, 36, 17-28.
14. Cherry, R. S., & Papoutsakie, E. T. (1986). Hydrodynamic effect on cells in agitated tissue culture reactors. Bioprocess Engineering, l, 29-41.
15. Rs, Cherry., & Papoutsakie, E. T. (1988). Physical mechanism of cell damage in microcarrier cell culture in cell culture bioreactors. Biotechnology and Bioengineering, 321, 2002-2004.
16. Freed, L. E., Vunjak-Novakovic, G., & Langer, R. (1993). Cultivation of cell-polymer cartilage implants in bioreactors. Journal of Cellular Biochemistry, 51, 257-264.
17. Martin, I., Padera, R. F., Vunjak-Novakovic, G., & Freed, L. E. (1998). In vitro differentiation of chick embryo bone marrow stromal cells into cartilaginous and bone like tissues. Journal of Orthopaedic Research, 16, 181-189.
18. Vunjak-Novakovic, G., Martin, I., Obradovic, B., Treppo, S., Grodzinsky, A. J., Langer, R., et al. (1999). Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage. Journal of Orthopaedic Research, 17, 130-138.
19. Kadiyala, S., Young, R. G., Thiede, M. A., & Bruder, S. P. (1997). Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro. Cell Transplantation, 6, 125-134.
20. Johnstone, B., Hering, T. M., Caplan, A. I., Goldberg, V. M., & Yoo, J. U. (1998). In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells. Experimental Cell Research, 238, 265-272.
21. Wang, W. G., Lou, S. Q., Ju, X. D., Xia, K., & Xia, J. H. (2003). In vitro chondrogenesis of human bone marrow-derived mesenchymal progenitor cells in monolayer culture: Activation by transfection with TGF-beta2. Tissue and Cell, 35, 69-77.
22. Cho, J. H., Kim, S.-H., Park, K. D., & Lee, J. W. (2004). Chondrogenic differentiation of human mesenchymal stem cells using a thermosensitive poly(N-isopropylaeyrlamide) and water-soluble chitosan copolymer. Biomaterials, 25, 5743-5751.
23. Freed, L. E., Martin, I., & Vunjak-Novakovic, G. (1999). Frontiers in tissue engineering: In vitro modulation of chondrogenesis. Journal of Clinical Orthodontics, 367S, 46-58.
24. Bayrr, F., Boynton, R. E., Liu, B., & Muprhy, J. M. (2001). Chondrogenie differentiation of mesenchymal stem cells from bone marrow: Differentiation-dependent gene expression of matrix components. Experimental Cell Research, 268, 189-200.