Three-dimensional culture of rat calvarial osteoblasts in porous biodegradable polymers

Biomaterials

Volumn 19, Issue 15, 1998, Pages 1405-1412

  Ishaug Riley, Susan L ^a,c   Crane Kruger, Genevieve M ^a,d   Yaszemski, Michael J ^b   Mikos, Antonios G ^a  

^a RICE UNIVERSITY   (United States)

^b MAYO CLINIC   (United States)

^c Advanced Tissue Sciences Liquidating Trust   (United States)

^d UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Biodegradable polymer scaffolds; Bone tissue engineering; Osteoblasts; PLGA; Poly( hydroxy esters); Poly(DL lactic co glycolic acid); Three dimensional culture

Indexed keywords

ANIMAL CELL CULTURE; BIOASSAY; BIODEGRADATION; DNA; GROWTH KINETICS; POLYMERS; POROUS MATERIALS;

CELL SEEDING DENSITY; POLYMER SCAFFOLDS; RAT CALVARIAL OSTEOBLASTS;

BIOMATERIALS;

ALKALINE PHOSPHATASE; POLYGLACTIN; POLYMER;

ANIMAL CELL; ARTICLE; CALVARIA; CELL ADHESION; CELL CULTURE; CELL DENSITY; CELL PROLIFERATION; DNA CONTENT; MINERALIZATION; NEWBORN; NONHUMAN; OSTEOBLAST; PRIORITY JOURNAL; RAT; TIME;

NASA DISCIPLINE CELL BIOLOGY; NON-NASA CENTER;

ALKALINE PHOSPHATASE; ANIMALS; BIOCOMPATIBLE MATERIALS; BONE DEVELOPMENT; BONE MATRIX; CELL ADHESION; CELL COUNT; CELL DIVISION; CELLS, CULTURED; LACTIC ACID; OSTEOBLASTS; POLYGLYCOLIC ACID; POLYMERS; RATS; RATS, SPRAGUE-DAWLEY;

ANIMALIA;

EID: 0032144180     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0142-9612(98)00021-0     Document Type: Article

References (16)

1. [1] Ishaug SL, Yaszemski MJ, Bizios R, Mikos AG. Osteoblast function on synthetic biodegradable polymers. J Biomed Mater Res 1994;28:1445-53.
2. [2] Ishaug SL, Payne RG, Yaszemski MJ, Aufdemorte TB, Bizios R, Mikos AG. Osteoblast migration on poly(α-hydroxy esters). Biotechnol Bioengng 1996;50:443-51.
3. [3] Ishaung SL, Crane GM, Miller MJ, Yasko AW, Yaszemski MJ, Mikos AG. Bone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffolds. J Biomed Mater Res 1997;36:17-28.
4. [4] Ishaug-Riley SL, Crane GM, Gurlek A, Miller MJ, Yasko AW, Yaszemski MJ, Mikos AG. Ectopic bone formation by marrow stromal osteoblast transplantation using poly(DL-lactic-co-glycolic acid) foams implanted into the rat mesentery. J Biomed Mater Res 1997;36:1-8.
5. [5] Mikos AG, Thorsen AJ, Czerwonka LA, Bao Y, Langer R, Winslow DN, Vacanti JP. Preparation and characterization of poly (L-lactic acid) foams. Polymer 1994;35:1068-77.
6. [6] Mikos AG, Lyman MD, Freed LE, Langer R. Wetting of poly(L-lactic acid) and poly(DL-lactic-co-glycolic acid) foams for tissue culture. Biomaterials 1994;15:55-8.
7. [7] Bellows CG, Aubin JE, Heersche JNM. Physiological concentration of glucocorticoids stimulate formation of bone nodules from isolated rat calvaria cells in vitro. Endocrin 1987;121:1985-92.
8. [8] Pockwinse SM, Stein JL, Lian JB, Stein GS. Developmental stage-specific cellular responses to vitamin D and glucocorticoids during differentiation of the osteoblast phenotype: interrelationship of morphology and gene expression by in situ hybridization. Exper Cell Res 1995;216:244-60.
9. [9] Shalhoub V, Conlon D, Tassinari M, Quinn C, Partridge N, Stein G, Lian J. Glucocorticoids promote development of the osteoblast phenotype by selectively modulating expression of cell growth and differentiation associated genes. J Cell Biochem 1992; 50:425-40.
10. [10] West DC, Sattar A, Kumar S. A simplified in situ solubilization procedure for the determination of DNA and cell number in tissue cultured mammalian cells. Anal Biochem 1985;147:289-95.
11. [11] Ishaug-Riley SL. Bone formation by three-dimensional osteoblast culture in biodegradable poly(α-hydroxy ester) scaffolds. PhD Thesis, Rice University, 1997.
12. [12] Lian JB, Stein GS. Concepts of osteoblast growth and differentiation: basis for modulation of bone cell development and tissue formation. Crit Rev Oral Biol Med 1992;3:269-305.
13. [13] Pockwinse SM, Wilming LG, Conlon DM, Stein GS, Lian JB. Expression of cell growth and bone specific genes at single cell resolution during development of bone tissue-like organization of primary osteoblast cultures. J Cell Biochem 1992;49:310-23.
14. [14] Cheng SL, Yang JW, Rifas L, Zhang SF, Avioli LV. Differentiation of human bone marrow osteogenic stromal cells in vitro: induction of the osteoblast phenotype by dexamethasone. Endocrin 1994;134:277-86.
15. [15] Puleo DA, Holleran LA, Doremus RH, Bizios R. Osteoblast responses to orthopedic implant materials in vitro. J Biomed Mater Res 1991;25:711-23.
16. [16] Mikos AG, Sarakinos G, Lyman MD, Ingber DE, Vacanti JP, Langer R. Prevascularization of porous biodegradable polymers. Biotechnol Bioengng 1993;42:716-23.