Sensitive and specific quantitative detection of rotavirus a by one-step real-time reverse transcription-PCR assay without antecedent double-stranded-RNA denaturation

Journal of Clinical Microbiology

Volumn 51, Issue 9, 2013, Pages 3047-3054

  Mijatovic Rustempasic, Slavica ^a   Tam, Ka Ian ^a   Kerin, Tara K ^a,b   Lewis, Jamie M ^a   Gautam, Rashi ^a   Quaye, Osbourne ^a,c   Gentsch, Jon R ^a   Bowen, Michael D ^a  

^a NATIONAL CENTER FOR IMMUNIZATION AND RESPIRATORY DISEASES   (United States)

^b UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^c UNIVERSITY OF GHANA   (Ghana)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED RNA; RECOMBINANT ENZYME; RECOMBINANT THERMOSTABLE THERMUS THERMOPHILUS POLYMERASE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DENATURATION; FECES ANALYSIS; GENE; GENE DOSAGE; GENOME ANALYSIS; GENOTYPE; LIMIT OF DETECTION; MEASUREMENT PRECISION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NSP3 GENE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA DENATURATION; RNA TRANSCRIPTION; ROTAVIRUS; SENSITIVITY AND SPECIFICITY; STRAIN IDENTIFICATION; VIRUS DETECTION; VIRUS GENOME; VIRUS LOAD; VIRUS STRAIN;

FECES; HUMANS; NUCLEIC ACID DENATURATION; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, DOUBLE-STRANDED; ROTAVIRUS; SENSITIVITY AND SPECIFICITY; VIRAL LOAD;

EID: 84882755332     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01192-13     Document Type: Article

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