Measurement of In vitro microtubule polymerization by turbidity and fluorescence

Methods in Cell Biology

Volumn 115, Issue , 2013, Pages 215-229

  Mirigian, Matthew ^a   Mukherjee, Kamalika ^b   Bane, Susan L ^b   Sackett, Dan L ^a  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^b BINGHAMTON UNIVERSITY   (United States)

Author keywords

Critical concentration; Fluorescence; Microtubules; Optical density; Polymerization; Tubulin; Turbidity; Turbidity coefficient

Indexed keywords

FLUORESCENT DYE; TUBULIN;

ANIMAL; ARTICLE; BRAIN; CATTLE; CHEMISTRY; CRITICAL CONCENTRATION; CYTOLOGY; DRUG EFFECT; FLUORESCENCE; METABOLISM; METHODOLOGY; MICROTUBULE; OPTICAL DENSITY; PHOTOMETRY; POLYMERIZATION; RAT; SPECTROPHOTOMETRY; TURBIDITY; TURBIDITY COEFFICIENT;

CRITICAL CONCENTRATION; FLUORESCENCE; MICROTUBULES; OPTICAL DENSITY; POLYMERIZATION; TUBULIN; TURBIDITY; TURBIDITY COEFFICIENT;

ANIMALS; BRAIN; CATTLE; FLUORESCENCE; FLUORESCENT DYES; MICROTUBULES; NEPHELOMETRY AND TURBIDIMETRY; POLYMERIZATION; RATS; SPECTROPHOTOMETRY; TUBULIN;

EID: 84882720882     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-407757-7.00014-1     Document Type: Chapter

References (23)

1. Andreu J.M. Large scale purification of brain tubulin with the modified Weisenberg procedure. Methods in Molecular Medicine 2007, 137:17-28.
2. Andreu J.M., Timasheff S.N. Tubulin bound to colchicine forms polymers different from microtubules. Proceedings of the National Academy of Sciences of the United States of America 1982, 79:6753-6756.
3. Bane S.L., Ravindra R., Zaydman A.A. High-throughput screening of microtubule-interacting drugs. Methods in Molecular Medicine 2007, 137:81-88.
4. Barron D.M., Chatterjee S.K., Ravindra R., Roof R., Baloglu E., Kingston D.G., et al. A fluorescence-based high-throughput assay for antimicrotubule drugs. Analytical Biochemistry 2003, 315:49-56.
5. Berne B.J. Interpretation of the light scattering from long rods. Journal of Molecular Biology 1974, 89:755-758.
6. Bollag D.M., McQueney P.A., Zhu J., Hensens O., Koupal L., Liesch J., et al. Epothilones, a new class of microtubule-stabilizing agents with a taxol-like mechanism of action. Cancer Research 1995, 55:2325-2333.
7. Bonne D., Heuséle C., Simon C., Pantaloni D. 4',6-Diamidino-2-phenylindole, a fluorescent probe for tubulin and microtubules. The Journal of Biological Chemistry 1985, 260:2819-2825.
8. Correia J.J., Williams R.C. Mechanisms of assembly and disassembly of microtubules. Annual Review of Biophysics and Bioengineering 1983, 12:211-235.
9. Davis A., Martinez S., Nelson D., Middleton K. A tubulin polymerization microassay used to compare ligand efficacy. Methods in Cell Biology 2010, 95:331-351.
10. Detrich H.W., Jordan M.A., Wilson L., Williams R.C. Mechanism of tubulin assembly. Changes in polymer structure and organization during assembly of sea urchin tubulin. The Journal of Biological Chemistry 1985, 260:9479-9490.
11. Gaskin F. Analysis of microtubule assembly kinetics using turbidimetry. Methods in Molecular Biology 2011, 777:99-105.
12. Gaskin F., Cantor C.R., Shelanski M.L. Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubules. Journal of Molecular Biology 1974, 89:737-755.
13. Hall D., Minton A.P. Turbidity as a probe of tubulin polymerization kinetics: A theoretical and experimental re-examination. Analytical Biochemistry 2005, 345:198-213.
14. Hamel E. Evaluation of antimitotic agents by quantitative comparisons of their effects on the polymerization of purified tubulin. Cell Biochemistry and Biophysics 2003, 38:1-22.
15. Hamel E., Lin C.M. Glutamate-induced polymerization of tubulin: Characteristics of the reaction and application to the large-scale purification of tubulin. Archives of Biochemistry and Biophysics 1981, 209:29-40.
16. Heusele C., Bonne D., Carlier M.F. Is microtubule assembly a biphasic process? A fluorimetric study using 4',6-diamidino-2-phenylindole as a probe. European Journal of Biochemistry 1987, 165:613-620.
17. Himes R.H., Burton P.R., Gaito J.M. Dimethyl sulfoxide-induced self-assembly of tubulin lacking associated proteins. The Journal of Biological Chemistry 1977, 252:6222-6228.
18. Lee J.C., Timasheff S.N. The reconstitution of microtubules from purified calf brain tubulin. Biochemistry 1975, 14:5183-5187.
19. Sackett D.L. Natural osmolyte trimethylamine N-oxide stimulates tubulin polymerization and reverses urea inhibition. The American Journal of Physiology 1997, 273:R669-R676.
20. Sackett D.L., Knipling L., Wolff J. Isolation of microtubule protein from mammalian brain frozen for extended periods of time. Protein Expression and Purification 1991, 2:390-393.
21. Sackett D.L., Werbovetz K.A., Morrissette N.S. Isolating tubulin from nonneural sources. Methods in Cell Biology 2010, 95:7-32.
22. Vater W., Böhm K.J., Unger E. Effects of the fluorescence dye DAPI on microtubule structure in vitro: Formation of novel types of tubulin assembly products. Acta Histochemica 1993, 94:54-66.
23. Weisenberg R.C. Microtubule formation in vitro in solutions containing low calcium concentrations. Science 1972, 177:1104-1105.