Natural osmolyte trimethylamine N-oxide stimulates tubulin polymerization and reverses urea inhibition

American Journal of Physiology - Regulatory Integrative and Comparative Physiology

Volumn 273, Issue 2 42-2, 1997, Pages

  Sackett, Dan L ^a,b  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

Betaine; Methylamine; Microtubules

Indexed keywords

BETAINE; DIMETHYLGLYCINE; GLYCINE; METHYLAMINE; PODOPHYLLOTOXIN; SARCOSINE; TRIMETHYLAMINE OXIDE; TUBULIN;

ANIMAL TISSUE; ARTICLE; MICROTUBULE; NONHUMAN; OSMOLARITY; OSMOTIC PRESSURE; OSMOTIC STRESS; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN PROTEIN INTERACTION; RAT; UREA CYCLE;

EID: 0030805303     PISSN: 03636119     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpregu.1997.273.2.r669     Document Type: Article

References (13)

1. Arakawa, T., and S. N. Timasheff. The mechanism of action of Na glutamate, lysine HCl, and piperazine-N,N′-bis(2-ethanesulfonic acid) in the stabilization of tubulin and microtubule formation. J. Biol. Chem. 259: 4979-4984, 1984.
2. Brown, A., and J. Simpson. Water relations of sugar-tolerant yeasts. The role of intracellular polyols. J. Gen. Microbiol. 72: 589-591, 1972.
3. Burg, M. B. Molecular basis of osmotic regulation. Am. J. Physiol. 268 (Renal Fluid Electrolyte Physiol. 37): F983-F996, 1995.
4. Clark, M. E. The osmotic role of amino acids: discovery, and function. In: Transport Processes, Iono- and Osmoregulation, edited by R. Gilles and M. Gilles-Baillien. Berlin: Springer-Verlag, 1985, p. 412-423.
5. Detrich, H. W., III, M. A. Jordon, L. Wilson, and R. C. Williams, Jr. Mechanism of microtubule assembly. J. Biol. Chem. 260: 9479-9490, 1985.
6. Detrich, H. W., III, V. Prasad, and R. F. Luduena. Cold-stable microtubules from antarctic fishes contain unique alpha tubulins. J. Biol. Chem. 262: 8360-8366, 1987.
7. Erickson, H. P., and W. A. Voter. Polycation-induced assembly of purified tubulin. Proc. Natl. Acad. Sci. USA 73: 2813-2817, 1976.
8. Garcia-Perez, A., and M. B. Burg. Importance of organic osmolytes for osmoregulation by renal medullary cells. Hypertension 16: 595-602, 1990.
9. Hamel, E., A. A. del Campo, M. C. Lowe, P. G. Waxman, and C. M. Lin. Effects of organic acids on tubulin polymerization and associated guanosine 5′-triphosphate hydrolysis. Biochemistry 21: 503-509, 1982.
10. Hamel, E., and C. M. Lin. Separation of active tubulin and microtubule-associated proteins by ultracentrifugation and isolation of a component causing the formation of microtubule bundles. Biochemistry 23: 4173-4184, 1984.
11. Herzog, W., and K. Weber. Microtubule formation by pure brain tubulin in vitro. The influence of dextran and poly(ethylene glycol). Eur. J. Biochem. 91: 249-254, 1978.
12. Himes, R. H., P. R. Burton, and J. M. Gaito. Dimethyl sulfoxide-induced self-assembly of tubulin lacking associated proteins. J. Biol. Chem. 252: 6222-6228, 1977.
13. Kita, Y., T. Arakawa, T.-Y. Lin, and S. N. Timasheff. Contribution of the surface free energy perturbation to protein-solvent interactions. Biochemistry 33: 15178-15189, 1994.