High-resolution HPLC-ESI-MS characterization of the contact sites of the actin-thymosin β4 complex by chemical and enzymatic cross-linking

Biochemistry

Volumn 52, Issue 33, 2013, Pages 5553-5562

  Knop, Jana ^a   App, Christine ^a   Horn, Anselm H C ^a   Iavarone, Federica ^b   Castagnola, Massimo ^b,c   Hannappel, Ewald ^a  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

^b CATHOLIC UNIVERSITY   (Italy)

^c CNR   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL CROSS-LINKING; COMPLEX FORMATIONS; CONTACT SITES; CROSS LINKING AGENTS; CROSSLINKING REACTION; HIGH RESOLUTION; INGEL DIGESTION; MICROBIAL TRANSGLUTAMINASE;

AMINO ACIDS; PROTEINS;

CROSSLINKING;

1 (3 DIMETHYLAMINOPROPYL) 3 ETHYLCARBODIIMIDE; ACTIN; AMINO ACID; CYANAMIDE; HISTIDINE; IMIDAZOLE; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; THYMOSIN BETA4; TRYPSIN;

ANIMAL TISSUE; ARTICLE; COMPLEX FORMATION; COVALENT BOND; CROSS LINKING; ELECTROSPRAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR MODEL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; REACTION ANALYSIS;

ACTINS; ANIMALS; BINDING SITES; CATTLE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CROSS-LINKING REAGENTS; CRYSTALLOGRAPHY, X-RAY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MULTIPROTEIN COMPLEXES; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; THYMOSIN; TRANSGLUTAMINASES;

EID: 84882573192     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi400664k     Document Type: Article

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