Comparative assessment of 5′ A/T-rich overhang sequences with optimal and sub-optimal primers to increase PCR yields and sensitivity

Molecular Biotechnology

Volumn 55, Issue 1, 2013, Pages 17-26

  Arif, M ^a   Ochoa Corona, F M ^a  

^a OKLAHOMA STATE UNIVERSITY   (United States)

Author keywords

5 A T rich flap; Diagnostics; DNA amplification; Primer design; qPCR; Tobacco mosaic virus

Indexed keywords

COMPARATIVE ASSESSMENT; CYCLING CONDITIONS; DNA AMPLIFICATION; OLIGONUCLEOTIDE PRIMERS; PRIMER DESIGN; QPCR; THERMODYNAMICS PARAMETERS; TOBACCO MOSAIC VIRUS;

DESIGN; DNA; DNA SEQUENCES; OLIGONUCLEOTIDES; PLASMA DIAGNOSTICS; POLYMERASE CHAIN REACTION; THERMODYNAMICS; TOBACCO; VIRUSES; WORLD WIDE WEB;

OPTIMIZATION;

ADENOSINE; PLANT VIRUS MOVEMENT PROTEIN; PRIMER DNA; THYMIDINE;

AMINO ACID SEQUENCE; ARTICLE; COMPUTER PROGRAM; CONTROLLED STUDY; FLUORESCENCE ANALYSIS; GENE AMPLIFICATION; GENE TARGETING; MOLECULAR CLONING; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; THERMODYNAMICS; TOBACCO MOSAIC VIRUS; VIRUS GENE;

TOBACCO MOSAIC VIRUS;

EID: 84882451678     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-012-9617-5     Document Type: Article

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