Design and construction of a single tube, quantitative endpoint, LATE-PCR multiplex assay for ventilator-associated pneumonia

Journal of Applied Microbiology

Volumn 115, Issue 3, 2013, Pages 818-827

  Rice, L M ^a   Reis, A H ^a   Mistry, R ^b   Khan, H ^b   Khosla, P ^b   Bharya, S ^b   Wangh, L J ^a  

^a BRANDEIS UNIVERSITY   (United States)

^b Smiths Detection Diagnostics   (United Kingdom)

Author keywords

Endpoint; LATE PCR; Low temperature molecular beacon probes; Multiplex assay; VAP

Indexed keywords

BACTERIA; DIAGNOSIS; FLUOROPHORES; GENES; PATHOGENS; PROBES; TEMPERATURE;

ENDPOINT; EXPONENTIALS; LINEAR-AFTER-THE-EXPONENTIAL POLYMERASE CHAIN REACTION; LOW-TEMPERATURE MOLECULAR BEACON PROBE; LOWS-TEMPERATURES; MOLECULAR BEACON; MULTIPLEX ASSAYS; QUANTITATIVE DETECTION; SINGLE TUBES; VENTILATOR-ASSOCIATED PNEUMONIA;

POLYMERASE CHAIN REACTION;

DOUBLE STRANDED DNA; GENOMIC DNA;

AMPLIFICATION; ANTIBIOTIC RESISTANCE; BIOASSAY; DNA; FLUORESCENCE; GENOMICS; HYBRIDIZATION; PATHOGEN; PNEUMONIA; POLYMERASE CHAIN REACTION;

ACINETOBACTER BAUMANNII; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; GENE AMPLIFICATION; GENE SEQUENCE; HYBRIDIZATION; LACTOCOCCUS LACTIS; LIMIT OF DETECTION; LIMIT OF QUANTITATION; LINEAR AFTER THE EXPONENTIAL POLYMERASE CHAIN REACTION; LOW TEMPERATURE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MOLECULAR BEACON; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA; STAPHYLOCOCCUS AUREUS; VENTILATOR ASSOCIATED PNEUMONIA;

ACINETOBACTER BAUMANNII; LACTOCOCCUS LACTIS; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; PSEUDOMONAS AERUGINOSA;

ENDPOINT; LATE-PCR; LOW-TEMPERATURE MOLECULAR BEACON PROBES; MULTIPLEX ASSAY; VAP;

ACINETOBACTER BAUMANNII; HUMANS; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; MULTIPLEX POLYMERASE CHAIN REACTION; NUCLEIC ACID HYBRIDIZATION; PNEUMONIA, VENTILATOR-ASSOCIATED; PSEUDOMONAS AERUGINOSA;

EID: 84881662474     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/jam.12281     Document Type: Article

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