Design and optimization of a novel reverse transcription linear-after-the-exponential PCR for the detection of foot-and-mouth disease virus

Journal of Applied Microbiology

Volumn 109, Issue 1, 2010, Pages 180-189

  Pierce, K E ^a   Mistry, R ^b   Reid, S M ^c   Bharya, S ^b   Dukes, J P ^c   Hartshorn, C ^a   King, D P ^c   Wangh, L J ^a  

^a MA 02454   (United States)

^b Bushey   (United Kingdom)

^c INSTITUTE FOR ANIMAL HEALTH   (United Kingdom)

Author keywords

Armored RNA; Asymmetric PCR; Diagnosis; Foot and mouth disease; LATE PCR; Real time RT PCR; RNA viruses

Indexed keywords

ASSAYS; POLYMERASE CHAIN REACTION; RNA; TRANSCRIPTION; VIRUSES;

ARMORED RNA; ASYMMETRIC PCR; EXPONENTIALS; FOOT AND MOUTH DISEASE; LINEAR-AFTER-THE-EXPONENTIAL POLYMERASE CHAIN REACTION; REAL- TIME; REAL-TIME REVERSE TRANSCRIPTION-PCR; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION PCR; RNA VIRUS;

DIAGNOSIS;

COMPLEMENTARY DNA; SYNTHETIC DNA; VIRUS RNA;

BIOASSAY; DETECTION METHOD; GENETIC ANALYSIS; OPTIMIZATION; PATHOGEN; POLYMERASE CHAIN REACTION; VIRUS;

ARTICLE; CONSENSUS SEQUENCE; CONTROLLED STUDY; FLUORESCENCE; FOOT AND MOUTH DISEASE; FOOT AND MOUTH DISEASE VIRUS; HEPATITIS C VIRUS; MOLECULAR PROBE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROCESS OPTIMIZATION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION LINEAR AFTER THE EXPONENTIAL POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; VIRUS DETECTION; VIRUS STRAIN;

FOOT-AND-MOUTH DISEASE VIRUS; RNA VIRUSES;

EID: 77953562685     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2009.04640.x     Document Type: Article

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