Pilot-Scale Recovery of Phycoerythrin from Porphyridium cruentum using Expanded Bed Adsorption Chromatography

Separation Science and Technology (Philadelphia)

Volumn 48, Issue 13, 2013, Pages 1913-1922

  Bermejo, Ruperto ^a   Ruiz, Esmeralda ^a   Ramos, Amparo ^a   Acién, F Gabriel ^b  

^a UNIVERSITY OF JAÉN   (Spain)

^b UNIVERSITY OF ALMERÍA   (Spain)

Author keywords

anion exchange chromatography; B phycoerythrin; expanded bed adsorption chromatography; phycobiliproteins; Porphyridium cruentum; preparative protein purification

Indexed keywords

ANION EXCHANGE CHROMATOGRAPHY; B-PHYCOERYTHRIN; EXPANDED BED ADSORPTION; PHYCOBILIPROTEINS; PORPHYRIDIUM CRUENTUM; PROTEIN PURIFICATION;

CELL CULTURE; OPTIMIZATION; PACKED BEDS; PROTEINS; PURIFICATION; RECOVERY;

CHROMATOGRAPHY;

EID: 84881652461     PISSN: 01496395     EISSN: 15205754     Source Type: Journal    
DOI: 10.1080/01496395.2013.791319     Document Type: Article

References (31)

1. Glazer, A.N. (1999) Phycobiliproteins. In: Chemicals from Microalgae, Cohen, Z.ed.; Taylor and Francic: UK, 262 - 280.
2. Glazer, A.N. (1994) Phycobiliproteins: A family of valuable, widely used fluorophores. J. Appl. Phycol., 6: 105 - 112.
3. Chowdhury, M.H., Ray, K., Aslan, K., Lakowicz, J.R., Geddes, C.D. (2007) Metal-enhanced fluorescence of phycobiliproteins from heterogeneous plasmonic nanostructures. J. Phys. Chem., 111: 18856 - 18863.
4. Arad, S. and Yaron, A. (1992) Natural pigments from red microalgae for use in foods and cosmetics. Trends in Food Sci. Technol., 3 (C): 92 - 97.
5. Ayyagari, M., Pande, R., Kamtekar, S., Gao, H., Marx, K., Kumar, J., Tripathy, S.K., Akkara, J.A., Kaplan, D.L. (1995) Molecular assembly of proteins and conjugated polymers: Toward development of biosensors. Biotech. Bioeng., 45: 116 - 121.
6. Bermejo, R., Fernández, E., Alvarez-Pez, J.M., Talavera, E.M. (2002) Labeling of cytosine residues with biliproteins for use as fluorescent DNA probes. J. Lumines., 99: 113 - 124.
7. Bermejo, R., Talavera, E.M., Alvarez-Pez, J.M., Orte, J.C. (1997) Chromatographic purification of biliproteins from Spirulina platensis. High-performance liquid chromatographic separation of their α and β subunits. J. Chrom. A, 778 (1-2): 441 - 450.
8. Bermejo Román, R., Alvárez-Pez, J.M., Acién Fernández, F.G., Molina Grima, E. (2002) Recovery of pure B-phycoerythrin from the microalga Porphyridium cruentum. J. Biotechnol., 93 (1): 73 - 85.
9. Chase, H.A. (1994) Purification of proteins by adsorption chromatography in expanded beds. Trends Biotech., 12 (8): 296 - 303.
10. Clemmitt, R.H., Chase, H.A. (2000) Facilitated downstream processing of a histidine-tagged protein from unclarified E. coli homogenates using immobilized metal affinity expanded-bed adsorption. Biotech. Bioeng., 67 (2): 206 - 216.
11. Anspach, F.B., Curbelo, D., Hartmann, R., Garke, G., Deckwer, W. (1999) Expanded bed chromatography in primary protein purification. J. Chrom. A, 855: 129 - 144.
12. Wang, G. (2002) Isolation and purification of phycoerythrin from red alga Gracilaria verrucosa by expanded-bed-adsorption and ion-exchange chromatography. Chromatographia, 56: 509 - 513.
13. Niu, J., Wang, G., Tseng, C. (2006) Method for large-scale isolation and purification of R-phycoerythrin from red alga Polysiphonia urceolata Grev. Protein Expression and Purification, 49: 23 - 31.
14. Niu, J., Wang, G., Zhou, B., Lin, X., Chen, C. (2007) Purification of R-phycoerythrin from Porphyra haitanensis using expanded-bed adsorption. J. Phycol., 43: 1339 - 1347.
15. Niu, J., Chen, Z., Wang, G., Zhou, B. (2010) Purification of phycoerythrin from Porphyra yezoensis Ueda using expanded bed adsorption. J. Appl. Phycol., 22: 25 - 31.
16. Bermejo, R., Acién, F.G., Ibáñez, M.J., Fernaández, J.M., Molina, E., Alvarez-Pez, J.M. (2003) Preparative purification of B-phycoerythrin from the microalga Porphyridium cruentum by expanded-bed adsorption chromatography. J. Chrom. B, 790 (1-2): 317 - 325.
17. Bermejo, R., Felipe, M.A., Talavera, E.M., Alvarez-Pez, J.M. (2006) Expanded bed adsorption chromatography for recovery of phycocyanins from the microalga Spirulina platensis. Chromatographia, 63 (1-2): 59 - 66.
18. Niu, J., Wang, G., Lin, X., Zhou, B. (2007) Large-scale recovery of C-phycocyanin from Spirulina platensis using expanded bed adsorption chromatography. J. Chrom. B, 850 (1-2): 267-276.
19. Ramos, A., Acién, F.G., Fernández-Sevilla, J.M., González, C.V., Bermejo, R. (2010) Large-scale isolation and purification of c-phycocyanin from the cyanobacteria Anabaena marina using expanded bed adsorption chromatography. J Chem Technol Biotechnol, 85: 783 - 792.
20. Ramos, A., Acién, F.G., Fernández-Sevilla, J.M., González, C.V., Bermejo, R. (2011) Development of a process for large-scale purification of C-phycocyanin from Synechocystis aquatilis using expanded bed adsorption chromatography. J. Chrom. B, 879: 511 - 519.
21. Bermejo, R., Ramos, A. (2012) Pilot-scale recovery of phycocyanin from Spirulina platensis using expanded bed adsorption chromatography. Chromatographia, 75: 195 - 204.
22. Hemerick, G. (1973) Culture Methods and Growth Measurements. In Handbook of Physiological Methods, Cambridge University Press: UK. 259 - 260.
23. Camacho, F., Acién, F.G., Sánchez, J.A., García, F., Molina, E. (1999) Prediction of dissolved oxygen and carbon dioxide concentration profiles in tubular photobioreactors for microalgal culture. Biotech. Bioeng., 62 (1): 71 - 86.
24. Bennet, A., Bogorad, L. (1973) Complementary chromatic adaptation in a filamentous blue-green algae. J. Cell. Biol., 58: 419 - 422.
25. Bryant, D.A., Guglielmi, G., Tandeu de Marsac, N., Castets, A.M., Cohen-Bazire, G. (1979) The structure of cyanobacterial phycobilisomes: A model. Arch. Microbiol., 123: 113 - 127.
26. Glazer, A.N. (1988) Phycobiliproteins. Meth. Enzymol., 167: 291 - 303.
27. Shepard, S.R., Boyd, G.A., Schrimsher, J.L. (2001) Routine manufacture of recombinant proteins using expanded bed adsorption chromatography. Bioseparation, 10: 51 - 56.
28. Rosano, R., Húngaro, N., D'Ambrosio, A., Liuzzi, G.M., Riccio, P. (2003) Extracting and purifying R-phycocyanin from Mediterranean red algae Corallina elongata. J Biotechnol, 101: 289 - 293.
29. Bermejo, R., Ruiz, E., Acien, F.G. (2007) Recovery of B-phycoerythrin using expanded bed adsorption chromatography: Scale-up of the process. Enzyme and Microbial Technol., 40: 927 - 933.
30. Sidler, W., Kumpf, B., Suter, F., Klotz, A.V., Glazer, A.N., Zuber, H. (1989) The complete aminoacid sequence of the alpha and beta subunits of B-phycoerythrin from the rhodophytan alga Porphyridium cruentum. Biological Chemistry Hoppe-Seyler, 370: 115 - 124.
31. Ficner, R., Lobeck, K., Schmidt, G., Huber, R. (1992) Isolation, crystallization, crystal structure analysis and refinement of B-phycoerythrin from the red alga Porphyridium sordidum at 2'2 Å resolution. J. Mol. Biol., 228: 935 - 950.