Development of a process for large-scale purification of C-phycocyanin from Synechocystis aquatilis using expanded bed adsorption chromatography

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 879, Issue 7-8, 2011, Pages 511-519

  Ramos, Amparo ^a   Acién, F Gabriel ^b   Fernández Sevilla, José M ^b   González, Cynthia V ^b   Bermejo, Ruperto ^a  

^a UNIVERSITY OF JAÉN   (Spain)

^b UNIVERSITY OF ALMERÍA   (Spain)

Author keywords

Anion exchange chromatography; C phycocyanin; Expanded bed adsorption chromatography; Phycobiliproteins; Preparative protein purification; Synechocystis aquatilis

Indexed keywords

ANION EXCHANGE CHROMATOGRAPHY; C-PHYCOCYANIN; EXPANDED BED ADSORPTION; PHYCOBILIPROTEINS; PREPARATIVE PROTEIN PURIFICATION; SYNECHOCYSTIS;

ABSORPTION SPECTROSCOPY; ADSORPTION; CELL CULTURE; CELLULOSE; EMISSION SPECTROSCOPY; ION EXCHANGE; IONS; OPTIMIZATION; PROTEINS; PURIFICATION; RECOVERY;

COLUMN CHROMATOGRAPHY;

C PHYCOCYANIN; CELLULOSE; PHYCOBILIPROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELL SUSPENSION; CONTROLLED STUDY; DRUG ISOLATION; DRUG PURIFICATION; EXPANDED BED CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; NONHUMAN; OSMOTIC STRESS; PH; PRIORITY JOURNAL; SCALE UP; SYNECHOCYSTIS; SYNECHOCYSTIS AQUATILIS;

ADSORPTION; CHROMATOGRAPHY, DEAE-CELLULOSE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; PHYCOCYANIN; SPECTROMETRY, FLUORESCENCE; SYNECHOCYSTIS; VISCOSITY;

CYANOBACTERIA; SYNECHOCYSTIS AQUATILIS;

EID: 79951682330     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2011.01.013     Document Type: Article

References (36)

1. Glazer A.N. Methods Enzymol. 1988, 167:291.
2. Glazer A.N. Chemicals from Microalgae 1999, Taylor and Francis Ltd., UK, p. 262.
3. Kronick M.N., Grossman P.D. Clin. Chem. 1983, 29(9):1582.
4. Glazer A.N. J. Appl. Phycol. 1994, 6(2):105.
5. Glazer A.N. Methods Enzymol. 1984, 106:359.
6. Chowdhury M.H., Ray K., Aslan K., Lakowicz J.R., Geddes C.D. J. Phys. Chem. C 2007, 111(51):18856.
7. Arad S., Yaron A. Trends Food Sci. Technol. 1992, 3:92.
8. Ayyagari M.S., Pande R., Kamtekar S., Gao H., Marx K.A., Kumar J. Biotechnol. Bioeng. 1995, 45(2):116.
9. Bermejo R., Tobaruela D., Talavera E.M., Orte A., Alvarez-Pez J.M. J. Colloid Interface Sci. 2003, 263(2):616.
10. Remirez D., González A., Merino N., González R., Ancheta O., Romay C.C. Drug Dev. Res. 1999, 48(2):70.
11. Rimbau V., Camins A., Romay C., González R., Pallás M. Neurosci. Lett. 1999, 276(2):75.
12. Benedetti S., Rinalducci S., Benvenuti F., Francogli S., Pagliarani S., Giorgi L. J. Chromatogr. B 2006, 833(1):12.
13. Bhat V.B., Madyastha K.M. Biochem. Biophys. Res. Commun. 2000, 275(1):20.
14. Rimbau V., Camins A., Pubill D., Sureda F.X., Romay C., Gonzálezs R. Naunyn Schmiedebergs Arch. Pharmacol. 2001, 364(2):96.
15. Ficner R., Lobeck K., Schmidt G., Huber R. J. Mol. Biol. 1992, 228(3):935.
16. Bermejo R., Talavera E.M., Alvarez-Pez J.M., Orte J.C. J. Chromatogr. A 1997, 778(1-2):441.
17. Bermejo Román R., Alvárez-Pez J.M., Acién Fernández F.G., Molina Grima E. J. Biotechnol. 2002, 93(1):73.
18. Bermejo R., Acién F.G., Ibáñez M.J., Fernández J.M., Molina E., Alvarez-Pez J.M. J. Chromatogr. B 2003, 790(1-2):317.
19. Boussiba S., Richmond A.E. Arch. Microbiol. 1979, 120(2):155.
20. Herrera A., Boussiba S., Napoleone V., Hohlberg A. J. Appl. Phycol. 1989, 1(4):325.
21. Silveira S.T., De Menezes L.K., Burkert C.A.V., Kalil S.J. Bioprocess Biosyst. Eng. 2008, 31(5):477.
22. Chase H.A. Trends Biotechnol. 1994, 12(8):296.
23. Clemmitt R.H., Chase H.A. Biotechnol. Bioeng. 2000, 67(2):206.
24. Bermejo R., Felipe M.A., Talavera E.M., Alvarez-Pez J.M. Chromatographia 2006, 63(1-2):59.
25. Niu J., Wang G., Lin X., Zhou B. J. Chromatogr. B 2007, 850(1-2):267.
26. Siegelman H., Kycia J.H. Algal Biliproteins 1978, Cambridge University Press, London, p. 71.
27. Laemmli U.K. Nature 1970, 277:680.
28. Campanella L., Crescentini G., Avino P., Angiello L. Ann. Chim. 2000, 90(3-4):153.
29. Ng M.Y.T., Tan W.S., Abdullah N., Ling T.C., Tey B.T. J. Chromatogr. A 2007, 1172(1):47.
30. Rosano R., Húngaro N., D'Ambrosio A., Liuzzi G.M., Riccio P. J. Biotechnol. 2003, 101:289.
31. Barnfield A.K., Hjorth R., Hammarstrom A. Biotechnol. Bioeng. 1994, 44(8):922.
32. Chase H.A., Draeger N.M. Sep. Sci. Technol. 1992, 27(14):2021.
33. Ramos A., Acién F.G., Fernández-Sevilla J.M., González C.V., Bermejo R. J. Chem. Technol. Biotechnol. 2010, 85:783.
34. Duerring M., Schmidt G.B., Huber R. J. Mol. Biol. 1991, 217(3):577.
35. Santiago-Santos M.C., Ponce-Noyola T., Olvera-Ramírez R., Ortega-López J., Cañizares-Villanueva R.O. Process Biochem. 2004, 39(12):2047.
36. Mimuro M., Rümbeli R., Füglistaller P., Zuber H. BBA Bioenergetics 1986, 851(3):447.