Discrimination of Enterobacteriaceae and non-fermenting gram negative bacilli by MALDI-TOF mass spectrometry

Open Microbiology Journal

Volumn 7, Issue 1, 2013, Pages 118-122

  Schaumann, Reiner ^a   Knoop, Nicolas ^a   Genzel, Gelimer H ^a   Losensky, Kevin ^a   Rosenkranz, Christiane ^a   Stîngu, Catalina S ^a   Schellenberger, Wolfgang ^a   Rodloff, Arne C ^a   Eschrich, Klaus ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

E. coli; Ehec; Enterobacteriaceae; Epec; Maldi tof ms; Salmonella spp.; Shigella sonnei

Indexed keywords

ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; ENTEROBACTER AEROGENES; ENTEROBACTER CLOACAE; ENTEROBACTERIACEAE; ENTEROHEMORRHAGIC ESCHERICHIA COLI; ENTEROPATHOGENIC ESCHERICHIA COLI; GRAM NEGATIVE BACTERIUM; KLEBSIELLA PNEUMONIAE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MEASUREMENT ACCURACY; NON FERMENTING GRAM NEGATIVE BACTERIUM; NONHUMAN; PRIORITY JOURNAL; SALMONELLA ENTERICA; SALMONELLA ENTERITIDIS; SALMONELLA TYPHIMURIUM; SERRATIA MARCESCENS; SHIGELLA SONNEI; SPECIES DIFFERENCE; STRAIN IDENTIFICATION; VALIDATION STUDY;

EID: 84881363462     PISSN: None     EISSN: 18742858     Source Type: Journal    
DOI: 10.2174/1874285801307010118     Document Type: Article

References (17)

1. Holland RD, Wilkes JG, Rafii F, et al. Rapid identification of intact whole bacteria based on spectral patterns using matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 1996; 10: 1227-1232.
2. Krishnamurthy T, Ross PL, Rajamani U. Detection of pathogenic and non-pathogenic bacteria by matrix-assisted laser desorp-tion/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 1996; 10: 883-888.
3. Demirev PA, Ho YP, Ryzhov V, Fenselau C. Microorganism identification by mass spectrometry and protein database searches. Anal Chem 1999; 71: 2732-2738.
4. Fenselau C, Demirev PA. Characterization of intact microorganisms by MALDI mass spectrometry. Mass Spectrom Rev 2001; 20: 157-171.
5. Dieckmann R, Helmuth R, Erhard M, Malorny B. Rapid classification and identification of salmonellae at the species and subspecies levels by whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry. Appl Environ Microbiol 2008; 74: 7767-7778.
6. Mellmann A, Cloud J, Maier T, et al. Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria. J Clin Microbiol 2008; 46: 1946-1954.
7. Seng P, Drancourt M, Gouriet F, et al. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 2009; 49: 543-551.
8. Cherkaoui A, Hibbs J, Emonet S, et al. Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrome-try methods with conventional phenotypic identification for routine identification of bacteria to the species level. J Clin Microbiol 2010; 48: 1169-1175.
9. He Y, Li H, Lu X, Stratton CW, Tang Y. Mass spectrometry bio-typer system identifies enteric bacterial pathogens directly from colonies grown on selective stool culture media. J Clin Microbiol 2010; 48: 3888-3892.
10. Benagli C, Rossi V, Dolina M, Tonolla M, Petrini O, Ahmed N. Matrix-assisted laser desorption ionization-time of flight mass spectrometry for the identification of clinically relevant bacteria. PLoS ONE 2011; 6: e16424.
11. Karger A, Ziller M, Bettin B, Mintel B, Schares S, Geue L. Determination of serotypes of Shiga toxin-producing Escherichia coli isolates by intact cell matrix-assisted laser desorption ionization-time of flight mass spectrometry. Appl Environ Microbiol 2011; 77: 896-905.
12. O'Hara CM. Manual and automated instrumentation for identification of Enterobacteriaceae and other aerobic gram-negative bacilli. Clin Microbiol Rev 2005; 18: 147-162.
13. Rupf S, Breitung K, Schellenberger W, Merte K, Kneist S, Eschrich K. Differentiation of mutans streptococci by intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Oral Microbiol Immunol 2005; 20: 267-273.
14. Friedrichs C, Rodloff AC, Chhatwal GS, Schellenberger W, Eschrich K. Rapid identification of viridans streptococci by mass spectrometric discrimination. J Clin Microbiol 2007; 45: 2392-2397.
15. Schaumann R, Knoop N, Genzel GH, et al. A step towards the discrimination of beta-lactamase-producing clinical isolates of En-terobacteriaceae and Pseudomonas aeruginosa by MALDI-TOF mass spectrometry. Med Sci Monit 2012; 18: MT71-MT77.
16. Ackermann G, Tang YJ, Kueper R, et al. Resistance to moxiflox-acin in toxigenic Clostridium difficile isolates is associated with mutations in gyrA. Antimicrob Agents Chemother 2001; 45: 2348-2353.
17. Hauswaldt SI, Fickweiler K, Rodloff AC. Enteropathogene E. coli: Epidemiologie in Sachsen und Evaluation diagnostischer Metho-den. Mikrobiologie 2009; 16: 206-216.