Dipyridamole-related enhancement of gap junction coupling in the GM-7373 aortic endothelial cells correlates with an increase in the amount of connexin 43 mRNA and protein as well as gap junction plaques

Journal of Bioenergetics and Biomembranes

Volumn 45, Issue 4, 2013, Pages 409-419

  Begandt, Daniela ^a   Bader, Almke ^a   Gerhard, Linda ^a   Lindner, Julia ^a   Dreyer, Lutz ^a   Schlingmann, Barbara ^a,b   Ngezahayo, Anaclet ^a,b  

^a LEIBNIZ UNIVERSITY HANNOVER   (Germany)

^b CENTER FOR SYSTEMS NEUROSCIENCE   (Germany)

Author keywords

cAMP; Connexin 43; Dipyridamole; Endothelial cells; Gap junction

Indexed keywords

ANTIBODY; BREFELDIN A; CONNEXIN 43; CYCLIC AMP; DIPYRIDAMOLE; MESSENGER RNA;

ANIMAL CELL; ANTIBODY DETECTION; AORTA; AORTA ATHEROSCLEROSIS; ARTICLE; BLOOD BRAIN BARRIER; CONCENTRATION RESPONSE; CONTROLLED STUDY; DIFFUSION; ENDOTHELIUM CELL; GAP JUNCTION; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; INCUBATION TIME; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

CELLS, CULTURED; CONNEXIN 43; DIPYRIDAMOLE; ENDOTHELIAL CELLS; GAP JUNCTIONS; HUMANS; PHOSPHORYLATION; RNA, MESSENGER;

EID: 84881314749     PISSN: 0145479X     EISSN: 15736881     Source Type: Journal    
DOI: 10.1007/s10863-013-9518-8     Document Type: Article

References (47)

1. Atkinson MM, Lampe PD, Lin HH, Kollander R, Li XR, Kiang DT (1995) Cyclic AMP modifies the cellular distribution of connexin43 and induces a persistent increase in the junctional permeability of mouse mammary tumor cells. J Cell Sci 108(Pt 9):3079-3090
2. Beardslee MA, Laing JG, Beyer EC, Saffitz JE (1998) Rapid turnover of connexin43 in the adult rat heart. Circ Res 83(6):629-635 (Pubitemid 28427746)
3. Begandt D, Bintig W, Oberheide K, Schlie S, Ngezahayo A (2010) Dipyridamole increases gap junction coupling in bovine GM-7373 aortic endothelial cells by a cAMP-protein kinase A dependent pathway. J Bioenerg Biomembr 42(1):79-84
4. Begandt D, Bader A, Dreyer L, Eisert N, Reeck T, Ngezahayo A (2013) Biphasic increase of gap junction coupling induced by dipyridamole in the rat aortic A-10 vascular smooth muscle cell line. J Cell Commun Signal 7(2):151-161
5. Berthoud VM, Minogue PJ, Guo J, Williamson EK, Xu X, Ebihara L, Beyer EC (2003) Loss of function and impaired degradation of a cataract-associated mutant connexin50. Eur J Cell Biol 82(5):209-221 (Pubitemid 36656567)
6. Bintig W, Begandt D, Schlingmann B, Gerhard L, Pangalos M, Dreyer L, Hohnjec N, Couraud PO, Romero IA, Weksler BB, Ngezahayo A (2011) Purine receptors and Ca(2+) signalling in the human blood-brain barrier endothelial cell line hCMEC/D3. Purinergic Signal
7. Bruzzone R, White TW, Paul DL (1996) Connections with connexins: the molecular basis of direct intercellular signaling. Eur J Biochem 238(1):1-27 (Pubitemid 26164180)
8. Chadjichristos CE, Kwak BR (2007) Connexins: new genes in atherosclerosis. Ann Med 39(6):402-411
9. Chanson M, White MM, Garber SS (1996) cAMP promotes gap junctional coupling in T84 cells. Am J Physiol 271(2 Pt 1):C533-C539
10. Civitelli R, Ziambaras K, Warlow PM, Lecanda F, Nelson T, Harley J, Atal N, Beyer EC, Steinberg TH (1998) Regulation of connexin43 expression and function by prostaglandin E2 (PGE2) and parathyroid hormone (PTH) in osteoblastic cells. J Cell Biochem 68(1):8-21
11. Cooper CD, Lampe PD (2002) Casein kinase 1 regulates connexin-43 gap junction assembly. J Biol Chem 277(47):44962-44968 (Pubitemid 36159094)
12. Cruciani V, Mikalsen SO (2002) Connexins, gap junctional intercellular communication and kinases. Biol Cell 94(7-8):433-443 (Pubitemid 36113695)
13. Diener HC, Cunha L, Forbes C, Sivenius J, Smets P, Loewenthal A (1996) European stroke prevention study. 2. Dipyridamole and acetylsalicylic acid in the secondary prevention of stroke. J Neurol Sci 143(1-2):1-13 (Pubitemid 26402780)
14. Eisert WG (2006) Dipyridamole. In: Michelson AD (ed) Platelets. Academic, Amsterdam, pp 1165-1179
15. ESPRIT Study Group, Halkes PH, van Gijn J, Kappelle LJ, Koudstaal PJ, Algra A (2006) Aspirin plus dipyridamole versus aspirin alone after cerebral ischaemia of arterial origin (ESPRIT): randomised controlled trial. 367, 9523
16. Faucheux N, Nagel MD (2002) Cyclic AMP-dependent aggregation of Swiss 3T3 cells on a cellulose substratum (Cuprophan) and decreased cell membrane Rho A. Biomaterials 23(11):2295-2301 (Pubitemid 34234830)
17. Figueroa XF, Isakson BE, Duling BR (2004) Connexins: gaps in our knowledge of vascular function. Physiology (Bethesda) 19:277-284 (Pubitemid 39472639)
18. Green CR, Nicholson LF (2008) Interrupting the inflammatory cycle in chronic diseases-do gap junctions provide the answer? Cell Biol Int 32(12):1578-1583
19. Guo S, Stins M, Ning M, Lo EH (2010) Amelioration of inflammation and cytotoxicity by dipyridamole in brain endothelial cells. Cerebrovasc Dis 30(3):290-296
20. Haefliger JA, Meda P (2000) Chronic hypertension alters the expression of Cx43 in cardiovascular muscle cells. Braz J Med Biol Res 33(4):431-438
21. Haefliger JA, Meda P, Formenton A, Wiesel P, Zanchi A, Brunner HR, Nicod P, Hayoz D (1999) Aortic connexin43 is decreased during hypertension induced by inhibition of nitric oxide synthase. Arterioscler Thromb Vasc Biol 19(7):1615-1622 (Pubitemid 29323819)
22. Harris AL (2001) Emerging issues of connexin channels: biophysics fills the gap. Q Rev Biophys 34(3):325-472 (Pubitemid 34123306)
23. Hoffmann A, Gloe T, Pohl U, Zahler S (2003) Nitric oxide enhances de novo formation of endothelial gap junctions. Cardiovasc Res 60(2):421-430 (Pubitemid 37412376)
24. Johnstone S, Isakson B, Locke D (2009) Biological and biophysical properties of vascular connexin channels. Int Rev Cell Mol Biol 278:69-118
25. Kumar NM, Gilula NB (1996) The gap junction communication channel. Cell 84(3):381-388 (Pubitemid 26060720)
26. Lampe PD, Cooper CD, King TJ, Burt JM (2006) Analysis of Connexin43 phosphorylated at S325, S328 and S330 in normoxic and ischemic heart. J Cell Sci 119(Pt 16):3435-3442 (Pubitemid 44405227)
27. Loewenstein WR (1981) Junctional intercellular communication: the cell-to-cell membrane channel. Physiol Rev 61(4):829-913
28. Maza J, Das Sarma J, Koval M (2005) Defining a minimal motif required to prevent connexin oligomerization in the endoplasmic reticulum. J Biol Chem 280(22):21115-21121 (Pubitemid 40805671)
29. Mehta PP, Yamamoto M, Rose B (1992) Transcription of the gene for the gap junctional protein connexin43 and expression of functional cell-to-cell channels are regulated by cAMP. Mol Biol Cell 3(8):839-850 (Pubitemid 23079758)
30. Molina-Arcas M, Casado FJ, Pastor-Anglada M (2009) Nucleoside transporter proteins. Curr Vasc Pharmacol 7(4):426-434
31. Murray SA, Shah US (1998) Modulation of adrenal gap junction expression. Horm Metab Res 30(6-7):426-431 (Pubitemid 28308022)
32. Musil LS, Goodenough DA (1991) Biochemical analysis of connexin43 intracellular transport, phosphorylation, and assembly into gap junctional plaques. J Cell Biol 115(5):1357-1374 (Pubitemid 21909980)
33. Olk S, Zoidl G, Dermietzel R (2009) Connexins, cell motility, and the cytoskeleton. Cell Motil Cytoskeleton 66(11):1000-1016
34. Podgorska M, Kocbuch K, Pawelczyk T (2005) Recent advances in studies on biochemical and structural properties of equilibrative and concentrative nucleoside transporters. Acta Biochim Pol 52(4):749-758 (Pubitemid 43155002)
35. Saffitz JE, Laing JG, Yamada KA (2000) Connexin expression and turnover. Circ Res 86(7):723-728
36. Salameh A, Krautblatter S, Karl S, Blanke K, Gomez DR, Dhein S, Pfeiffer D, Janousek J (2009) The signal transduction cascade regulating the expression of the gap junction protein connexin43 by beta-adrenoceptors. Br J Pharmacol 158(1):198-208
37. Sands WA, Palmer TM (2008) Regulating gene transcription in response to cyclic AMP elevation. Cell Signal 20(3):460-466
38. Solan JL, Lampe PD (2005) Connexin phosphorylation as a regulatory event linked to gap junction channel assembly. Biochim Biophys Acta 1711(2):154-163 (Pubitemid 40799290)
39. Solan JL, Lampe PD (2007) Key connexin 43 phosphorylation events regulate the gap junction life cycle. J Membr Biol 217(1-3):35-41 (Pubitemid 350160003)
40. Solan JL, Lampe PD (2009) Connexin43 phosphorylation: structural changes and biological effects. Biochem J 419(2):261-272
41. Somekawa S, Fukuhara S, Nakaoka Y, Fujita H, Saito Y, Mochizuki N (2005) Enhanced functional gap junction neoformation by protein kinase A-dependent and Epac-dependent signals downstream of cAMP in cardiac myocytes. Circ Res 97(7):655-662 (Pubitemid 41416334)
42. Sosinsky GE, Solan JL, Gaietta GM, Ngan L, Lee GJ, Mackey MR, Lampe PD (2007) The C-terminus of connexin43 adopts different conformations in the Golgi and gap junction as detected with structure-specific antibodies. Biochem J 408(3):375-385
43. Trosko JE, Chang CC, Wilson MR, Upham B, Hayashi T, Wade M (2000) Gap junctions and the regulation of cellular functions of stem cells during development and differentiation. Methods 20(2):245-264 (Pubitemid 30130816)
44. van Rijen HV, van Veen TA, Hermans MM, Jongsma HJ (2000) Human connexin40 gap junction channels are modulated by cAMP. Cardiovasc Res 45(4):941-951 (Pubitemid 30089656)
45. Wang Y, Rose B (1995) Clustering of Cx43 cell-to-cell channels into gap junction plaques: regulation by cAMP and microfilaments. J Cell Sci 108(Pt 11):3501-3508
46. Weksler BB, Subileau EA, Perriere N, Charneau P, Holloway K, Leveque M, Tricoire-Leignel H, Nicotra A, Bourdoulous S, Turowski P, Male DK, Roux F, Greenwood J, Romero IA, Couraud PO (2005) Blood-brain barrier-specific properties of a human adult brain endothelial cell line. FASEB J 19(13):1872-1874 (Pubitemid 41598779)
47. Yogo K, Ogawa T, Akiyama M, Ishida N, Takeya T (2002) Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells. FEBS Lett 531(2):132-136 (Pubitemid 35341183)