Lipid, detergent, and coomassie blue G-250 affect the migration of small membrane proteins in blue native gels: Mitochondrial carriers migrate as monomers not dimers

Journal of Biological Chemistry

Volumn 288, Issue 30, 2013, Pages 22163-22173

  Crichton, Paul G ^a   Harding, Marilyn ^a   Ruprecht, Jonathan J ^a   Lee, Yang ^a   Kunji, Edmund R S ^a  

^a MEDICAL RESEARCH COUNCIL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BLUE-NATIVE GELS; HIGH MOLECULAR MASS; MEMBRANE PROTEINS; MITOCHONDRIAL CARRIER; PROTEIN MONOMERS;

BIOLOGICAL MEMBRANES; GELS; MONOMERS;

DIMERS;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ANAZOLENE SODIUM; CARRIER PROTEIN; DETERGENT; DIMER; LIPID; MEMBRANE PROTEIN; MONOMER;

ARTICLE; CELL MIGRATION; ELECTROPHORETIC MOBILITY; GEL ELECTROPHORESIS; GEL FILTRATION; GEL PERMEATION CHROMATOGRAPHY; MITOCHONDRION; MOLECULAR WEIGHT; NONHUMAN; OXIDATIVE PHOSPHORYLATION; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN PURIFICATION; SOLUBILIZATION; ULTRACENTRIFUGATION; YEAST;

DETERGENT MICELLE; GEL ELECTROPHORESIS; MEMBRANE PROTEINS; MITOCHONDRIAL TRANSPORT; PROTEIN AGGREGATION; TRANSPORTERS;

BLOTTING, WESTERN; DETERGENTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; LIPIDS; MITOCHONDRIAL ADP, ATP TRANSLOCASES; MOLECULAR WEIGHT; PROTEIN MULTIMERIZATION; ROSANILINE DYES; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 84881237077     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M113.484329     Document Type: Article

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