A disulphide bond in the E2 enzyme Pex4p modulates ubiquitin-conjugating activity

Scientific Reports

Volumn 3, Issue , 2013, Pages

  Williams, Chris ^a,d   Van Den Berg, Marlene ^b   Stanley, Will A ^c   Wilmanns, Matthias ^a   Distel, Ben ^b  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b ACADEMIC MEDICAL CENTER   (Netherlands)

^c NEWCASTLE UNIVERSITY   (United Kingdom)

^d UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

DISULFIDE; PEX4 PROTEIN, S CEREVISIAE; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE PROTEIN; SOLUTION AND SOLUBILITY; UBIQUITIN;

CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ACTIVATION; ENZYMOLOGY; GENETICS; METABOLISM; OXIDATION REDUCTION REACTION; PROTEIN CONFORMATION; SACCHAROMYCES CEREVISIAE; SOLUTION AND SOLUBILITY; UBIQUITINATION; ARTICLE; PROTEIN BINDING;

DISULFIDES; ENZYME ACTIVATION; MODELS, MOLECULAR; OXIDATION-REDUCTION; PROTEIN BINDING; PROTEIN CONFORMATION; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SOLUTIONS; UBIQUITIN; UBIQUITINATION;

EID: 84881137314     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep02212     Document Type: Article

References (28)

1. Wiebel, F. F.& Kunau, W.H. The Pas2 protein essential for peroxisome biogenesis is related to ubiquitin-conjugating enzymes. Nature 359, 73-76 (1992).
2. Platta, H. W. et al. Ubiquitination of the peroxisomal import receptor Pex5p is required for its recycling. J Cell Biol 177, 197-204 (2007). (Pubitemid 46658631)
3. Williams, C., van den Berg, M., Sprenger, R. R. &Distel, B. A conserved cysteine is essential for Pex4p-dependent ubiquitination of the peroxisomal import receptor Pex5p. J Biol Chem 282, 22534-22543 (2007). (Pubitemid 47267315)
4. Carvalho, A. F. et al. Ubiquitination of mammalian Pex5p, the peroxisomal import receptor. J Biol Chem 282, 31267-31272 (2007). (Pubitemid 350044882)
5. Okumoto, K. et al. Cysteine ubiquitination of PTS1 receptor Pex5p regulates Pex5p recycling. Traffic 12, 1067-1083 (2011).
6. Williams, C. et al. Insights into ubiquitin-conjugating enzyme/co-activator interactions from the structure of the Pex4p:Pex22p complex. EMBO J 31, 391-402 (2012).
7. Koller, A. et al. Pex22p of Pichia pastoris, essential for peroxisomal matrix protein import, anchors the ubiquitin-conjugating enzyme, Pex4p, on the peroxisomal membrane. J Cell Biol 146, 99-112 (1999). (Pubitemid 29349967)
8. Wu, P. Y. et al. A conserved catalytic residue in the ubiquitin-conjugating enzyme family. EMBO J 22, 5241-5250 (2003).
9. Thornton, J. M. Disulphide bridges in globular proteins. J Mol Biol 151, 261-287 (1981).
10. Tu, B. P. & Weissman, J. S. Oxidative protein folding in eukaryotes: mechanisms and consequences. J Cell Biol 164, 341-346 (2004). (Pubitemid 38174761)
11. Nagahara, N. Intermolecular disulfide bond to modulate protein function as a redox-sensing switch. Amino Acids 41, 59-72 (2010).
12. Linke, K. & Jakob, U. Not every disulfide lasts forever: disulfide bond formation as a redox switch. Antioxid Redox Signal 5, 425-434 (2003). (Pubitemid 37087402)
13. Ananda, K., Nacharaju, P., Smith, P. K., Acharya, S. A. &Manjula, B. N. Analysis of functionalization of methoxy-PEG as maleimide-PEG. Anal Biochem 374, 231-242 (2008).
14. Anson, M. L. The reactions of iodine and iodoacetamide with native egg albumin. J Gen Physiol 23, 321-331 (1940).
15. Williams, C., van den Berg, M., Geers, E. & Distel, B. Pex10p functions as an E3 ligase for the Ubc4p-dependent ubiquitination of Pex5p. Biochem Biophys Res Commun 374, 620-624 (2008).
16. Reverter, D. & Lima, C. D. Insights into E3 ligase activity revealed by a SUMORanGAP1- Ubc9-Nup358 complex. Nature 435, 687-692 (2005). (Pubitemid 40825514)
17. Plafker, K. S. et al. The ubiquitin-conjugating enzyme UbcM2 can regulate the stability and activity of the antioxidant transcription factor Nrf2. J Biol Chem 285, 23064-23074 (2010).
18. Itoh, K. et al. An Nrf2/small Maf heterodimer mediates the induction of phase II detoxifying enzyme genes through antioxidant response elements. Biochem Biophys Res Commun 236, 313-322 (1997). (Pubitemid 27373291)
19. Tolbert, B. S. et al. The active site cysteine of ubiquitin-conjugating enzymes has a significantly elevated pKa: functional implications. Biochemistry 44, 16385-16391 (2005). (Pubitemid 41832023)
20. Bosanac, I. et al.Ubiquitin binding to A20 ZnF4 is required formodulation of NFkappaB signaling. Mol Cell 40, 548-557 (2010).
21. VanDemark, A. P., Hofmann, R. M., Tsui, C., Pickart, C. M. & Wolberger, C. Molecular insights into polyubiquitin chain assembly: crystal structure of the Mms2/Ubc13 heterodimer. Cell 105, 711-720 (2001). (Pubitemid 32635101)
22. Wenzel, D. M., Lissounov, A., Brzovic, P. S. & Klevit, R. E. UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids. Nature 474, 105-108 (2011).
23. Kabsch, W. Xds. Acta Crystallogr D Biol Crystallogr 66, 125-132 (2010).
24. Evans, P. Scaling and assessment of data quality. Acta Crystallogr D Biol Crystallogr 62, 72-82 (2006).
25. Vagin, A. & Teplyakov, A. Molecular replacement with MOLREP. Acta Crystallogr D Biol Crystallogr 66, 22-25 (2010).
26. Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr D Biol Crystallogr 66, 486-501 (2010).
27. Murshudov, G. N. et al. REFMAC5 for the refinement of macromolecular crystal structures. Acta Crystallogr D Biol Crystallogr 67, 355-367 (2011).
28. Chen, V. B. et al. MolProbity: all-atom structure validation for macromolecular crystallography. Acta Crystallogr D Biol Crystallogr 66, 12-21 (2010).