Characterization of new β-galactosidase from acidophilic fungus, Teratosphaeria acidotherma AIU BGA-1

Journal of Bioscience and Bioengineering

Volumn 116, Issue 3, 2013, Pages 293-297

  Isobe, Kimiyasu ^a   Yamashita, Miho ^a   Chiba, Serina ^a   Takahashi, Naomi ^a   Koyama, Takahumi ^b  

^a IWATE UNIVERSITY   (Japan)

^b Amano Enzyme Inc   (Japan)

Author keywords

Galactosidase; Acidophilic fungus; Lactase; Lactose; Teratosphaeria acidotherma

Indexed keywords

GALACTOSIDASES; LACTASE; LACTOSE; N-TERMINAL AMINO ACID SEQUENCE; OPTIMAL ACTIVITY; PHYSICOCHEMICAL CHARACTERISTICS; SMALL SUBUNITS; TERATOSPHAERIA ACIDOTHERMA;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; ENZYMES; HYDROLYSIS; PH;

SUGARS;

BETA GALACTOSIDASE; LACTOSE; RESIN;

AMINO ACID SEQUENCE; ARTICLE; CHROMATOGRAPHY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME STABILITY; FUNGUS; MOLECULAR WEIGHT; NONHUMAN; PH; PHYSICAL CHEMISTRY; PROTEIN HYDROLYSIS; TERATOSPHAERIA ACIDOTHERMA;

FUNGI; TERATOSPHAERIA;

ACIDOPHILIC FUNGUS; LACTASE; LACTOSE; TERATOSPHAERIA ACIDOTHERMA; Β-GALACTOSIDASE;

ASCOMYCOTA; BETA-GALACTOSIDASE; CHROMATOGRAPHY, AFFINITY; ENZYME STABILITY; GALACTOSE; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; LACTOSE; MOLECULAR WEIGHT; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84881090299     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2013.03.012     Document Type: Article

References (24)

1. Panesar P.S., Panesar R., Singh R.S., Kennedy J.F., Kumar H. Microbial production, immobilization and application of β-galactosidase. J.Chem. Technol. Biotechnol. 2006, 81:530-543.
2. Mbuyi-Kalala A., Schnek G.A., Léonis J. Separation and characterization of four enzyme forms of β-galactosidase from Saccharomyces lactis. Eur. J. Biochem. 1988, 178:437-443.
3. Cavaille D., Combes D. Characterization of β-galactosidase from Kluyveromyces lactis. Biotechnol. Appl. Biochem. 1995, 22:55-64.
4. Ladero M., Santos A., Garcia J.L., Carrascosa A.V., Pessela B.C.C., Garcia-Ochoa F. Studies on the activity and the stability of β-galactosidase from Thermus sp. strain T2 and from Kluyveromyces fragilis. Enzyme Microb. Technol. 2002, 30:392-405.
5. Kim C.S., Ji E.-S., Oh D.-K. Expression and characterization of Kluyveromyces fragilis β-galactosidase in Escherichia coli. Biotechnol. Lett. 2003, 25:1769-1774.
6. Nguyen T.-H., Splechtna B., Steinböck M., Kneifel W., Lettner H.P., Kulbe K.D., Haltrich D. Purification and characterization of two novel β-galactosidases from Lactobacillus reuteri. J.Agric. Food Chem. 2006, 54:4989-4998.
7. Gul-Guven R., Guven K., Poli A., Nicolaus B. Purification and some properties of a β-galactosidase from the thermoacidophilic Alicyclobacillus acidocaldarius subsp. rittmannii isolated from Antarctica. Enzyme Microb. Technol. 2007, 40:1570-1577.
8. Chen W., Chen H., Xia Y., Yang J., Zhao J., Tian F., Zhang H.P., Zhang H. Immobilization of recombinant thermostable β-galactosidase from Bacillus stearothermophilus for lactose hydrolysis in milk. J.Dairy Sci. 2009, 92:491-498.
9. Rhimi M., Boisson A., Dejob M., Boudebouze S., Maguin E., Haser R., Aghajari N. Efficient bioconversion of lactose in milk and whey: immobilization and characterization of a β-galactosidase from the dairy Streptococcus thermophilus LMD9 strain. Res. Microbiol. 2010, 161:515-525.
10. Widmer F., Leuba J.L. β-Galactosidase from Aspergillus niger, separation and characterization of three multiple forms. Eur. J. Biochem. 1978, 100:559-567.
11. Manzanares P., de Graff L.H., Visser J. Characterization of galactosidases from Aspergillus niger: purification of a novel α-galactosidase activity. Enzyme Microb. Technol. 1998, 22:383-390.
12. Hatzinikolaou D.G., Katsifas E., Mamma D., Karagouni A.D., Christakopoulos P., Kekos D. Modeling of the simultaneous hydrolysis-ultrafiltration of whey permeate by a thermostable β-galactosidase from Aspergillus niger. Biochem. Eng. J. 2005, 24:161-172.
13. Gonzalez R.R., Monsan P. Purification and some properties of β-galactosidase from Aspergillus fonsecaeus. Enzyme Microb. Technol. 1991, 13:349-352.
14. Nagy Z., Kiss T., Szentirmai A., Biro S. β-Galactosidase from Penicillium chrysogenum: production, purification, and characterization of the enzyme. Protein Expr. Purif. 2001, 21:24-29.
15. Takenishi S., Watanabe Y., Miwa T., Kobayashi R. Purification and some properties of β-galactosidase from Penicillium multicolor. Agric. Biol. Chem. 1983, 47:2533-2540.
16. Sharikh S.A., Khire J.M., Khan M.I. Characterization of a thermostable extracellular β-galactosidase from a thermophilic fungus Rhizomucor sp. Biochim. Biophys. Acta 1999, 1472:314-322.
17. Nakkharat P., Haltrich D. Purification and characterization of an intracellular enzyme with β-glucosidase and β-galactosidase activity from the thermophilic fungus Talaromyces thermophilus CBS 236.58. J.Biotechnol. 2006, 123:304-313.
18. O'Connell S., Walsh G. Application relevant studies of fungal β-galactosidase with potential application in the alleviation of lactose intolerance. Appl. Biochem. Biotechnol. 2008, 149:129-138.
19. Wang H., Luo H., Bai Y., Wang Y., Yang P., Shi P., Zhang W., Fan Y., Yao B. An acidophilic β-galactosidase from Bispora sp. MEY-1 with high lactose hydrolytic activity under simulated gastric conditions. J.Agric. Food Chem. 2009, 57:5535-5541.
20. Isobe K., Takahashi N., Chiba S., Yamashita M., Koyama T. Acidophilic fungus, Teratosphaeria acidotherma AIU BGA-1, produces multiple forms of intracellular β-galactosidase. J. Biosci. Bioeng. 2013, 116:171-174.
21. Laemmli U.K. Cleavage of structure proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
22. Nguyen T.-H., Splechtna B., Krasteva S., Kneifel W., Kulbe K.D., Divne C., Haltrich D. Characterization and molecular cloning of a heterodimeric β-galactosidase from the probiotic strain Lactobacillus acidophilus R22. FEMS Microbiol. Lett. 2007, 269:136-144.
23. Iqbal S., Nguyen T.-H., Nguyen T.T., Maischberger T., Haltrich D. β-Galactosidase from Lactobacillus plantarum WCFS1: biochemical characterization and formation of prebiotic galacto-oligosaccharides. Carbohydr. Res. 2010, 345:1408-1416.
24. Nguyen T.-H., Splechtna B., Yamabhai M., Haltrich D., Peterbauer C. Cloning and expression of the β-galactosidase from Lactobacillus reuteri in Escherichia coli. J.Biotechnol. 2007, 129:581-591.