Luciferase assay to study the activity of a cloned promoter DNA fragment

Methods in Molecular Biology

Volumn 977, Issue , 2013, Pages 65-78

  Solberg, Nina ^a   Krauss, Stefan ^a  

^a NONE

Author keywords

Dual Luciferase; Firefly; NSC; Promoter; Renilla; Transfection

Indexed keywords

DNA FRAGMENT; FIREFLY LUCIFERASE; RENILLA LUCIFERIN 2 MONOOXYGENASE; THYMIDINE KINASE;

ARTICLE; HERPES SIMPLEX VIRUS; LUCIFERASE ASSAY; MOLECULAR CLONING; NEURAL STEM CELL; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE; TRANSCRIPTION INITIATION; ANIMAL; BIOSYNTHESIS; CULTURE TECHNIQUE; ENZYME ASSAY; ENZYMOLOGY; GENETIC TRANSFECTION; GENETICS; MOUSE; MULTICELLULAR SPHEROID;

ANIMALS; CELL CULTURE TECHNIQUES; CLONING, MOLECULAR; ENZYME ASSAYS; GENES, REPORTER; LUCIFERASES, FIREFLY; LUCIFERASES, RENILLA; MICE; NEURAL STEM CELLS; PROMOTER REGIONS, GENETIC; SPHEROIDS, CELLULAR; THYMIDINE KINASE; TRANSFECTION;

MLCS; MLOWN;

EID: 84880991594     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-284-1_6     Document Type: Article

References (14)

1. Fan F, Wood K.V. (2007) Bioluminescent assays for high-throughput screening. Assay Drug Dev Technol 5: 127-136
2. Massoud TF, Paulmurugan R, De A., Ray P, Gambhir S.S. (2007) Reporter gene imaging of protein-protein interactions in living subjects. Curr Opin Biotechnol 18: 31-37
3. de Almeida P.E., van R Jr, Wu J.C. (2011) In vivo bioluminescence for tracking cell fate and function. Am J Physiol Heart Circ Physiol 301: H663-H671
4. Greer LF III, Szalay AA (2002) Imaging of light emission from the expression of luciferases in living cells and organisms: a review. Luminescence 17: 43-74
5. De W Jr, Wood K.V., Helinski DR, Deluca M. (1985) Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli. Proc Natl Acad Sci USA 82: 7870-7873
6. Ow DW, Wit DE Jr, Helinski DR, Howell SH, Wood KV, Deluca M. (1986) Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Science 234: 856-859
7. De W Jr, Wood K.V., Deluca M., Helinski DR, Subramani S. (1987) Firefly luciferase gene: structure and expression in mammalian cells. Mol Cell Biol 7: 725-737
8. Zhang W, Purchio AF, Chen K, Wu J., Lu L, Coffee R, Contag P.R., West D.B. (2003) A transgenic mouse model with a luciferase reporter for studying in vivo transcriptional regulation of the human CYP3A4 gene. Drug Metab Dispos 31: 1054-1064
9. Padmanabhan P, Otero J, Ray P., Paulmurugan R, Hoffman AR, Gambhir S.S., Biswal S., Ulaner G.A. (2006) Visualization of telomerase reverse transcriptase (hTERT) promoter activity using a trimodality fusion reporter construct. J Nucl Med 47: 270-277
10. Solberg N, Machon O, Krauss S. (2012) Characterization and functional analysis of the 5-flanking promoter region of the mouse Tcf3 gene. Mol Cell Biochem 360: 289-299
11. Zipser D, Lipsich L, Kwoh J. (1981) Mapping functional domains in the promoter region of the herpes thymidine kinase gene. Proc Natl Acad Sci USA 78: 6276-6280
12. Lorenz WW, McCann RO, Longiaru M, Cormier M.J. (1991) Isolation and expression of a cDNA encoding Renilla reniformis luciferase. Proc Natl Acad Sci USA 88: 4438-4442
13. van den Bout CJ, Machon O, Rosok O., Backman M, Krauss S. (2002) The mouse enhancer element D6 directs Cre recombinase activity in the neocortex and the hippocampus. Mech Dev 110: 179-182
14. Yin W, Xiang P, Li Q. (2005) Investigations of the effect of DNA size in transient transfection assay using dual luciferase system. Anal Biochem 346: 289-294