Validation of a real-time PCR based method for detection of clostridium botulinum types C, D and their mosaic variants C-D and D-C in a multicenter collaborative trial

Anaerobe

Volumn 22, Issue , 2013, Pages 31-37

  Woudstra, Cedric ^a   Skarin, Hanna ^b   Anniballi, Fabrizio ^c   Auricchio, Bruna ^c   De Medici, Dario ^c   Bano, Luca ^d   Drigo, Ilenia ^d   Hansen, Trine ^e   Löfström, Charlotta ^e   Hamidjaja, Raditijo ^f   van Rotterdam, Bart J ^f   Koene, Miriam ^g   Bäyon Auboyer, Marie Hélène ^h   Buffereau, Jean Philippe ^h   Fach, Patrick ^a  

^a French Agency for Food   (France)

^b NATIONAL VETERINARY INSTITUTE   (Sweden)

^c ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^d ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELLE VENEZIE   (Italy)

^e TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^f NATIONAL INSTITUTE FOR PUBLIC HEALTH AND THE ENVIRONMENT   (Netherlands)

^g WAGENINGEN UNIVERSITY   (Netherlands)

^h Analysis and Development Laboratory 22   (France)

Author keywords

Animal botulism; C.botulinum C and D; Evaluation trial; GeneDisc array

Indexed keywords

BACTERIAL DNA;

ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; BOTULISM; CLOSTRIDIUM BOTULINUM TYPE C; CLOSTRIDIUM BOTULINUM TYPE D; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DENMARK; FRANCE; ITALY; MULTICENTER STUDY; NETHERLANDS; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SWEDEN;

ANIMAL BOTULISM; C. BOTULINUM C AND D; EVALUATION TRIAL; GENEDISC(®) ARRAY;

ANIMALS; BOTULISM; CLOSTRIDIUM BOTULINUM TYPE C; CLOSTRIDIUM BOTULINUM TYPE D; EUROPE; HUMANS; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS;

ANIMALIA; AVES; CLOSTRIDIUM BOTULINUM; MAMMALIA;

EID: 84880731229     PISSN: 10759964     EISSN: 10958274     Source Type: Journal    
DOI: 10.1016/j.anaerobe.2013.05.002     Document Type: Article

References (26)

1. Hatheway C.L. Botulism: the present status of the disease. Curr Top Microbiol Immunol 1995, 195:55-75.
2. Popoff M.R. Ecology of neurotoxigenic strains of clostridia. Curr Top Microbiol Immunol 1995, 195:1-29.
3. Skarin H., Lindberg A., Blomqvist G., Aspan A., Baverud V. Molecular characterization and comparison of Clostridium botulinum type C avian strains. Avian Pathol 2010, 39:511-518.
4. Arnon S.S., Schechter R., Inglesby T.V., Henderson D.A., Bartlett J.G., Ascher M.S., et al. Botulinum toxin as a biological weapon: medical and public health management. JAm Med Assoc 2001, 285:1059-1070.
5. Wein L.M., Liu Y. Analyzing a bioterror attack on the food supply: the case of botulinum toxin in milk. Proc Natl Acad Sci U S A 2005, 102:9984-9989.
6. Knutsson R. Atracing tool portfolio to detect Bacillus anthracis, Clostridium botulinum and Noroviruses: bioterrorism is a food safety and security issue. Int J Food Microbiol 2011, 145(Suppl. 1):S121-S122.
7. CDC Clostridium botulinum. Monovalent and polyvalent antitoxins 1987, Center for Disease Control, Atlanta, Georgia.
8. Gimenez D.F., Gimenez J.A. The typing of botulinal neurotoxins. Int J Food Microbiol 1995, 27:1-9.
9. Woudstra C., Skarin H., Anniballi F., Fenicia L., Bano L., Drigo I., et al. Neurotoxin gene profiling of Clostridium botulinum types C and D native to different countries within Europe. Appl Environ Microbiol 2012, 78:3120-3127.
10. Nakamura K., Kohda T., Umeda K., Yamamoto H., Mukamoto M., Kozaki S. Characterization of the D/C mosaic neurotoxin produced by Clostridium botulinum associated with bovine botulism in Japan. Vet Microbiol 2010, 140:147-154.
11. Rossetto O., Morbiato L., Caccin P., Rigoni M., Montecucco C. Presynaptic enzymatic neurotoxins. JNeurochem 2006, 97:1534-1545.
12. Kirchner S., Kramer K.M., Schulze M., Pauly D., Jacob D., Gessler F., et al. Pentaplexed quantitative real-time PCR assay for the simultaneous detection and quantification of botulinum neurotoxin-producing clostridia in food and clinical samples. Appl Environ Microbiol 2010, 76:4387-4395.
13. Anniballi F., Auricchio B., Delibato E., Antonacci M., De Medici D., Fenicia L. Multiplex real-time PCR SYBR Green for detection and typing of group III Clostridium botulinum. Vet Microbiol 2012, 154:332-338.
14. Lindberg A., Skarin H., Knutsson R., Blomqvist G., Baverud V. Real-time PCR for Clostridium botulinum type C neurotoxin (BoNTC) gene, also covering a chimeric C/D sequence-application on outbreaks of botulism in poultry. Vet Microbiol 2010, 146:118-123.
15. Malorny B., Tassios P.T., Radstrom P., Cook N., Wagner M., Hoorfar J. Standardization of diagnostic PCR for the detection of foodborne pathogens. Int J Food Microbiol 2003, 83:39-48.
16. Bustin S.A., Benes V., Garson J.A., Hellemans J., Huggett J., Kubista M., et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 2009, 55:611-622.
17. Reddy N.R., Tetzloff R.C., Skinner G.E. Effect of media, additives, and incubation conditions on the recovery of high pressure and heat-injured Clostridium botulinum spores. Food Microbiol 2010, 27:613-617.
18. Iwasaki M., Ohishi I., Sakaguchi G. Evidence that botulinum C2 toxin has two dissimilar components. Infect Immun 1980, 29:390-394.
19. Fach P., Guillou J.P. Detection by invitro amplification of the alpha-toxin (phospholipase C) gene from Clostridium perfringens. JAppl Bacteriol 1993, 74:61-66.
20. Hill B.J., Skerry J.C., Smith T.J., Arnon S.S., Douek D.C. Universal and specific quantitative detection of botulinum neurotoxin genes. BMC Microbiol 2010, 10:267.
21. Kouguchi H., Suzuki T., Hasegawa K., Mutoh S., Watanabe T., Niwa K., et al. Quantitative detection of gene expression and toxin complex produced by Clostridium botulinum serotype D strain 4947. JMicrobiol Methods 2006, 67:416-423.
22. Langton S.D., Chevennement R., Nagelkerke N., Lombard B. Analysing collaborative trials for qualitative microbiological methods: accordance and concordance. Int J Food Microbiol 2002, 79:175-181.
23. Lindstrom M., Nevas M., Kurki J., Sauna-aho R., Latvala-Kiesila A., Polonen I., et al. Type C botulism due to toxic feed affecting 52,000 farmed foxes and minks in Finland. JClin Microbiol 2004, 42:4718-4725.
24. Payne J.H., Hogg R.A., Otter A., Roest H.I., Livesey C.T. Emergence of suspected type D botulism in ruminants in England and Wales (2001 to 2009), associated with exposure to broiler litter. Vet Rec 2011, 168:640.
25. Fenicia L., Fach P., van Rotterdam B.J., Anniballi F., Segerman B., Auricchio B., et al. Towards an international standard for detection and typing botulinum neurotoxin-producing Clostridia types A, B, E and F in food, feed and environmental samples: a European ring trial study to evaluate a real-time PCR assay. Int J Food Microbiol 2011, 145(Suppl. 1):S152-S157.
26. Hasan M.R., Tan R., Al-Rawahi G.N., Thomas E., Tilley P. Short-term stability of pathogen-specific nucleic acid targets in clinical samples. JClin Microbiol 2012, 50:4147-4150.